ID   A0A1I8G141_9PLAT        Unreviewed;       978 AA.
AC   A0A1I8G141;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE            EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
OS   Macrostomum lignano.
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC   Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX   NCBI_TaxID=282301 {ECO:0000313|Proteomes:UP000095280, ECO:0000313|WBParaSite:maker-uti_cns_0000468-snap-gene-0.1-mRNA-1};
RN   [1] {ECO:0000313|WBParaSite:maker-uti_cns_0000468-snap-gene-0.1-mRNA-1}
RP   IDENTIFICATION.
RG   WormBaseParasite;
RL   Submitted (NOV-2016) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000256|RuleBase:RU363044};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|RuleBase:RU363044};
CC   -!- SIMILARITY: Belongs to the helicase family.
CC       {ECO:0000256|RuleBase:RU363044}.
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DR   AlphaFoldDB; A0A1I8G141; -.
DR   WBParaSite; maker-uti_cns_0000468-snap-gene-0.1-mRNA-1; maker-uti_cns_0000468-snap-gene-0.1-mRNA-1; maker-uti_cns_0000468-snap-gene-0.1.
DR   Proteomes; UP000095280; Unplaced.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 2.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR031657; REPA_OB_2.
DR   PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR   PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR   Pfam; PF05970; PIF1; 1.
DR   Pfam; PF16900; REPA_OB_2; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|RuleBase:RU363044};
KW   DNA damage {ECO:0000256|RuleBase:RU363044};
KW   DNA recombination {ECO:0000256|RuleBase:RU363044};
KW   DNA repair {ECO:0000256|RuleBase:RU363044};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Helicase {ECO:0000256|RuleBase:RU363044};
KW   Hydrolase {ECO:0000256|RuleBase:RU363044};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   TRANSMEM        629..662
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        691..712
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          705..774
FT                   /note="OB"
FT                   /evidence="ECO:0000259|Pfam:PF01336"
FT   DOMAIN          811..894
FT                   /note="Replication protein A OB"
FT                   /evidence="ECO:0000259|Pfam:PF16900"
SQ   SEQUENCE   978 AA;  108525 MW;  5B39E8A5AAAC6BB7 CRC64;
     RYNLPTANEV GILIVKDTED TEAPEDGDEP ATSRDVVIQL RGLPHVHFLL WLVSGQRVTP
     DRYDKIVSAE IPDPQQQPRL HELVKTHMIH GPCGVVNPAC PCMVDGQCSK GFPKEFQQAT
     LTSDTGYPLY RRLSPEMGGF TVDKLFKGRS IRMDNSKAPT QAKKEGRNEI KEYLLARYIG
     PMEAVNTILG FEVRGPTSFE ALHTVDGVLC PSAKAACTAL GLLQRDDHWR RCLAEAVQSR
     MPRALRHLLA VILIWGNPED GSDLWCTFAE DLCEDLRFHH RDLSAEEITD LALRELQSML
     TSMGGHSLPE YGLPAASVPP QVGPMQFDPV EQAEIREARL SMLTEEQIAV YESVTADLVA
     GSGGLHFLDA PAGTGKTFLL EVLLAFVRSR GELALAVAAS GIAATLLPGG QTAHSTFKIP
     VRLLRSNKDV CAVGAQSKQA EVFRRVRLIV WDEISMTNRK DLESVDRCLR DVRKQDKPFG
     GVTLVCSGDF RQLLPVVVNG TRANSVMACV CRSSLWKLFK RHRLTRNIRL LCSSEPAMYQ
     KFTELLMQIG TGTIEQDAEE KVSLPAEMVI SAEAPLNDLI KFVYPSFEES AERDDYFASR
     AILAPYNDSV DAINETFNST NKLNLIIPLL LLLRLLLLLL LLLLLLLLLL LLLLLLLLLL
     LCCCCSRMSA RSGHRATGNM ESTEGAKKSK ACISALLPGL MHAAIVGLVM RLSKITDFEG
     KRGPGQVFSF ILCDDTGDIR VSVFGKDLKP FLEVCREGVA VRISGGWVKK ADPKYSTTDN
     EHELTVSIDQ RGTIVRTLET VTRRAPSYKK IKEISDCEMK MNVNVYGTIS AVQKPTEVKT
     GSGKLINLAK VTLVDQSGSS VSCTFWASST AQVNDWSVGD VLAIHGAQVQ EYSQTISLGC
     SKAEIQHWPE DSADVSDITQ WYKDALSSQQ CFEELRVTQS SQREKYANRD DEMVLQYPER
     MFDQFDEVRR IKVRFGDK
//