ID A0A1I8G4K8_9PLAT Unreviewed; 618 AA.
AC A0A1I8G4K8;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=BOX15_Mlig030936g1 {ECO:0000313|EMBL:PAA89900.1},
GN BOX15_Mlig030936g2 {ECO:0000313|EMBL:PAA89865.1}, BOX15_Mlig030936g4
GN {ECO:0000313|EMBL:PAA59392.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|Proteomes:UP000095280, ECO:0000313|WBParaSite:maker-uti_cns_0000865-snap-gene-0.3-mRNA-1};
RN [1] {ECO:0000313|WBParaSite:maker-uti_cns_0000865-snap-gene-0.3-mRNA-1, ECO:0000313|WBParaSite:maker-uti_cns_0046281-snap-gene-0.5-mRNA-1}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (NOV-2016) to UniProtKB.
RN [2] {ECO:0000313|EMBL:PAA59392.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA59392.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA59392.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
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DR EMBL; NIVC01002268; PAA59392.1; -; Genomic_DNA.
DR EMBL; NIVC01000133; PAA89865.1; -; Genomic_DNA.
DR EMBL; NIVC01000132; PAA89900.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I8G4K8; -.
DR STRING; 282301.A0A1I8G4K8; -.
DR WBParaSite; maker-uti_cns_0000865-snap-gene-0.3-mRNA-1; maker-uti_cns_0000865-snap-gene-0.3-mRNA-1; maker-uti_cns_0000865-snap-gene-0.3.
DR WBParaSite; maker-uti_cns_0046281-snap-gene-0.5-mRNA-1; maker-uti_cns_0046281-snap-gene-0.5-mRNA-1; maker-uti_cns_0046281-snap-gene-0.5.
DR Proteomes; UP000095280; Unplaced.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20342; BRcat_RBR_RNF217; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR InterPro; IPR047551; BRcat_RBR_RNF217.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR PANTHER; PTHR11685:SF478; E3 UBIQUITIN-PROTEIN LIGASE RNF217; 1.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 2.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 2.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT TRANSMEM 586..614
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 345..563
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 349..395
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 28..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 108..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..45
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..149
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 164..181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 618 AA; 69977 MW; C0FE66FF3D1113DB CRC64;
MTTEEEPVSL SLQEIRVADG CDQTVENEVT QLNPDTATVS DQSDSASDEE LADDLDLEQL
DVLAKQDTEE ALERLKMWVC NKSRKLSLSS QDREIFARLT EKYFSELKSL SSKEAEAEPG
TSSVVREADA VQSSSQPPSR TSSRTSSPLP MDPVRRRFAS DTSSEATRDM PVNVEGSDSA
SGSERSAKIL LFPEHAALFN DDSEEHLIML EALQNFLSEE RDYFHSISLI SLFKLFKYLL
MYHLESEYDF ESRSKSVNLS RTTDTLVFYK RLFDTGLAPH TFRQVYEKLT ETQSHLQINV
INDRITRWEN YHDIFMLHGM LTECFQVYQS RTGVVSGLER MNFRCSATCS LCLLDDQLVV
ARTCCGQHVC YACYNEWVRT KVYARTVEVG CPCCSCFLTV SEIIEFLGDR ADRYYEILTD
LNGDPNKKTC PQCNMVTKNP NEKASKRLGG ELTRCQRCEF SWCFRCHSPP HPNMSCSANS
SGKQMLRDWG LETYFDGSQF IRKARKCPSC KVYIEKNQGC NHMTCNRCSY QFCYVCGKNW
RAGNFVFGRH SSRLSIFGCK QKLFPGRPAL RKLVRGTLFV LEATGIVIAV PIGLALVIGL
SPILGVIGLG VVIYKCKH
//
