ID A0A1I8GWR2_9PLAT Unreviewed; 361 AA.
AC A0A1I8GWR2;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 {ECO:0000256|ARBA:ARBA00035038};
DE AltName: Full=Radical S-adenosyl methionine domain-containing protein 2 {ECO:0000256|ARBA:ARBA00035040};
DE AltName: Full=Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible {ECO:0000256|ARBA:ARBA00035042};
GN ORFNames=BOX15_Mlig012673g1 {ECO:0000313|EMBL:PAA73965.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|Proteomes:UP000095280, ECO:0000313|WBParaSite:maker-uti_cns_0003318-snap-gene-0.2-mRNA-1};
RN [1] {ECO:0000313|WBParaSite:maker-uti_cns_0003318-snap-gene-0.2-mRNA-1}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (NOV-2016) to UniProtKB.
RN [2] {ECO:0000313|EMBL:PAA73965.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA73965.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA73965.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004397}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004397}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004397}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. RSAD2 family.
CC {ECO:0000256|ARBA:ARBA00035008}.
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DR EMBL; NIVC01000984; PAA73965.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I8GWR2; -.
DR STRING; 282301.A0A1I8GWR2; -.
DR WBParaSite; maker-uti_cns_0003318-snap-gene-0.2-mRNA-1; maker-uti_cns_0003318-snap-gene-0.2-mRNA-1; maker-uti_cns_0003318-snap-gene-0.2.
DR Proteomes; UP000095280; Unplaced.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR026372; RSAD2.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04278; viperin; 1.
DR NCBIfam; NF038283; viperin_w_prok; 1.
DR PANTHER; PTHR21339; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2; 1.
DR PANTHER; PTHR21339:SF0; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01088; antiviral_proteins; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SFLD; SFLDF00318; Viperin; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Immunity {ECO:0000256|ARBA:ARBA00022859};
KW Innate immunity {ECO:0000256|ARBA:ARBA00022588};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 70..282
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 361 AA; 41186 MW; 7DD8E922D400F800 CRC64;
MPIVLFNNIE GSFPSSLITL VKIALTHLGH LGQLALANVR GFIFRRTLDA GRDTNDEAEK
PRAASAPPES VVPVSVNYHF TRRCNYKCGF CFHTAISSFV LPLEEAKRGL RMLQEAGMQK
VNFSGGEPFL EQRGRLLGEM VRFCKETLGL PSVSIVSNGS LITEEWFQTY GEWLDILAVS
CDSFDEATNR AIGRHAPRSH HLDKLYQISE WCARYRVAFK INTVVNTHNW QEDMADQILD
LNPCRWKVFQ CLLIGGENCG EDSLRHAETF VVTDEQFRSF LDRHSRVECL VPESNEKMRD
SYLILDERMR FLDCTRGRKE PSRSILDVGV ANAICASGFD EVMFRKRGGV YQWSKNDIME
W
//
