ID   A0A1I8J7F8_9PLAT        Unreviewed;       508 AA.
AC   A0A1I8J7F8;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE            EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN   ORFNames=BOX15_Mlig029351g2 {ECO:0000313|EMBL:PAA76297.1};
OS   Macrostomum lignano.
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC   Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX   NCBI_TaxID=282301 {ECO:0000313|Proteomes:UP000095280, ECO:0000313|WBParaSite:maker-uti_cns_0045997-snap-gene-1.25-mRNA-1};
RN   [1] {ECO:0000313|WBParaSite:maker-uti_cns_0045997-snap-gene-1.25-mRNA-1}
RP   IDENTIFICATION.
RG   WormBaseParasite;
RL   Submitted (NOV-2016) to UniProtKB.
RN   [2] {ECO:0000313|EMBL:PAA76297.1, ECO:0000313|Proteomes:UP000215902}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DV1 {ECO:0000313|EMBL:PAA76297.1};
RC   TISSUE=Whole organism {ECO:0000313|EMBL:PAA76297.1};
RA   Berezikov E.;
RT   "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT   model organism for stem cell research.";
RL   Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC         EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; NIVC01000831; PAA76297.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I8J7F8; -.
DR   STRING; 282301.A0A1I8J7F8; -.
DR   WBParaSite; maker-uti_cns_0045997-snap-gene-1.25-mRNA-1; maker-uti_cns_0045997-snap-gene-1.25-mRNA-1; maker-uti_cns_0045997-snap-gene-1.25.
DR   Proteomes; UP000095280; Unplaced.
DR   Proteomes; UP000215902; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR   CDD; cd20342; BRcat_RBR_RNF217; 1.
DR   Gene3D; 1.20.120.1750; -; 1.
DR   InterPro; IPR047551; BRcat_RBR_RNF217.
DR   InterPro; IPR031127; E3_UB_ligase_RBR.
DR   InterPro; IPR002867; IBR_dom.
DR   InterPro; IPR044066; TRIAD_supradom.
DR   PANTHER; PTHR11685:SF478; E3 UBIQUITIN-PROTEIN LIGASE RNF217; 1.
DR   PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR   Pfam; PF01485; IBR; 2.
DR   SMART; SM00647; IBR; 2.
DR   SUPFAM; SSF57850; RING/U-box; 3.
DR   PROSITE; PS51873; TRIAD; 1.
PE   4: Predicted;
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833};
KW   Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT   TRANSMEM        451..470
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        476..502
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          207..432
FT                   /note="RING-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51873"
FT   REGION          72..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        72..88
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   508 AA;  56617 MW;  482DDE5ED2D0ACE5 CRC64;
     MSSQVASLDE VSINSINIEA ASIESSPVDA EINEAVIASA LEIMDSPEQM AALSDEDRQR
     VLEITRNYFG EHRDSDVSET EERSSAGSEA TDDNLQSVMQ EASLALTMRL LHEELGDAMF
     ADLPRSSLFD IVHSIGKVAC ANGEPVVELL ECSSAANILP RLQAWPACHQ QLQDSAASSE
     FYNLFTASMH ELSERAGTRD ESEGEYRLGQ CDICCSEDDS LLWLGGCCQT SVCVFCLHRW
     LTDHLMEVGA KLGCPGCSAV LTVPQLRHLL SDSAMNRREQ LLARQRGGNS SSERDCPRCS
     CLTQRPSRRM RLRNGRWQKE HGGVTVKCAQ CDYVWCFDCH SPAHPGLSCR RFRSGVHLLE
     DWAKQLEQSG LRKARRCPKC KAMIEKNDGC NHMTCAHCEH EFCYLCRKRW RIPQLKIPYF
     RWSVFGSHSN RLSVFGCREK FLPNRPRLRR LVRGSLFVLR ILGLILVPTV GLSVLLAAAV
     ALVAVAIPLS PVIVAVVVTL TLKSHRVE
//