ID   A0A1I8MB60_MUSDO        Unreviewed;       895 AA.
AC   A0A1I8MB60;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 34.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   Name=101890677 {ECO:0000313|EnsemblMetazoa:MDOA003082-PA};
GN   Synonyms=LOC101890677 {ECO:0000313|RefSeq:XP_005188323.1};
OS   Musca domestica (House fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC   Muscidae; Musca.
OX   NCBI_TaxID=7370 {ECO:0000313|EnsemblMetazoa:MDOA003082-PA};
RN   [1] {ECO:0000313|EnsemblMetazoa:MDOA003082-PA}
RP   IDENTIFICATION.
RC   STRAIN=Aabys {ECO:0000313|EnsemblMetazoa:MDOA003082-PA};
RG   EnsemblMetazoa;
RL   Submitted (MAY-2020) to UniProtKB.
RN   [2] {ECO:0000313|RefSeq:XP_005188323.1}
RP   IDENTIFICATION.
RC   STRAIN=Aabys {ECO:0000313|RefSeq:XP_005188323.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   RefSeq; XP_005188323.1; XM_005188266.3.
DR   EnsemblMetazoa; MDOA003082-RA; MDOA003082-PA; MDOA003082.
DR   GeneID; 101890677; -.
DR   KEGG; mde:101890677; -.
DR   VEuPathDB; VectorBase:MDOA003082; -.
DR   eggNOG; KOG2119; Eukaryota.
DR   OrthoDB; 997839at2759; -.
DR   Proteomes; UP000095301; Unassembled WGS sequence.
DR   Proteomes; UP000694905; Unplaced.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 2.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000694905}.
FT   DOMAIN          87..393
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          453..883
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
SQ   SEQUENCE   895 AA;  102935 MW;  D50E57DC3C02E80E CRC64;
     MDSVSANYDE QQQRNEISAI PQYGLKLKFD HVWPEKRNQN LRPSIKQTLP LLPLACRYAA
     YYWKVSREGR RCYMDYYYTE HSKQMYGVPL GGIGGGTIGR GYAGEFCRFQ MRPGMYEYNT
     VQADQFIVTI KDVKGETIYQ SLLSKSNSSN NRPKQPLSSW QSNLDESKCS YTGLYPRSWT
     EYDLSKYGIR LVCRQISPVI PHDYKESSLP CAVFVWSVEN VGEEERTVSI TFTFKNGTGN
     KKQDAEGNAT SALFSEGNAK GVTINQVIAE MPCSYNLACR VLPEINITRC LKFDPNGSGE
     QLWQQLKDQG QLNDTAADET LKSNDLGVAI CARVSLKPKS QHDLEFALAW DMPTIHFPKK
     LKAHTRYYTK YFSANGESGP KICEYALKHY GNWEKLIDNW QRPILNDEGL PDWYKSAIFN
     QLYYISDGGT IWLLCDSSLG QDLEYNDPRT AYGRFGYLEG HEYRMYNTYD VHFYASPAIA
     HLWPNLQVSL QYDFKDAIAA NLTDTRKMLY DGKIMPRKVK NCVPHDLGDP DEEPFTLINC
     YNIHDVNDWK DLNTKFVLQV YRDYYVLNEL AQAQSDNASK FSSIEFIDKD SLYEMYSQDN
     KRKNSVEEKK ENRKSASMYI NETNGRVYLM DAMAYLKAMY PSCKAIMERT MEYDRDNDGL
     IENTKMPDQT YDSWVMEGPS AYCSGLWLAA LQSMSVMATI LDQPNDCIRY QDILEKGKRS
     LEEKLWNGSY YRFDTCNSHK DTIMADQLCG HWYLKTCGFD YEIYPKENVR SALKEIYKNN
     VMGFHNGNIG AANGFIANSD PEKPGHVDTS SIQSEEVWPG VTFALAATMI QEGMFEEGFK
     TAGGLYETIS EKCGMNFETP EAFYGEKRYR SIGYMRPLSI WSMQVAWERR KALRD
//