ID A0A1I8NEK3_MUSDO Unreviewed; 294 AA.
AC A0A1I8NEK3;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Enoyl-CoA hydratase, mitochondrial {ECO:0000256|ARBA:ARBA00041110};
DE EC=4.2.1.17 {ECO:0000256|ARBA:ARBA00012076};
DE EC=5.3.3.8 {ECO:0000256|ARBA:ARBA00012064};
DE AltName: Full=Enoyl-CoA hydratase 1 {ECO:0000256|ARBA:ARBA00042381};
DE AltName: Full=Short-chain enoyl-CoA hydratase {ECO:0000256|ARBA:ARBA00041421};
GN Name=101889661 {ECO:0000313|EnsemblMetazoa:MDOA014399-PA};
GN Synonyms=LOC101889661 {ECO:0000313|RefSeq:XP_005190041.1};
OS Musca domestica (House fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC Muscidae; Musca.
OX NCBI_TaxID=7370 {ECO:0000313|EnsemblMetazoa:MDOA014399-PA};
RN [1] {ECO:0000313|EnsemblMetazoa:MDOA014399-PA}
RP IDENTIFICATION.
RC STRAIN=Aabys {ECO:0000313|EnsemblMetazoa:MDOA014399-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
RN [2] {ECO:0000313|RefSeq:XP_005190041.1}
RP IDENTIFICATION.
RC STRAIN=Aabys {ECO:0000313|RefSeq:XP_005190041.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl-
CC CoA; Xref=Rhea:RHEA:31175, ChEBI:CHEBI:15377, ChEBI:CHEBI:62500,
CC ChEBI:CHEBI:62611; Evidence={ECO:0000256|ARBA:ARBA00036655};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31176;
CC Evidence={ECO:0000256|ARBA:ARBA00036655};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O;
CC Xref=Rhea:RHEA:26558, ChEBI:CHEBI:15377, ChEBI:CHEBI:57316,
CC ChEBI:CHEBI:57332; Evidence={ECO:0000256|ARBA:ARBA00036765};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:26560;
CC Evidence={ECO:0000256|ARBA:ARBA00036765};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O;
CC Xref=Rhea:RHEA:45584, ChEBI:CHEBI:15377, ChEBI:CHEBI:57332,
CC ChEBI:CHEBI:78611; Evidence={ECO:0000256|ARBA:ARBA00036643};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:45586;
CC Evidence={ECO:0000256|ARBA:ARBA00036643};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxyisovaleryl-CoA = 3-methyl-(2E)-butenoyl-CoA + H2O;
CC Xref=Rhea:RHEA:31079, ChEBI:CHEBI:15377, ChEBI:CHEBI:57344,
CC ChEBI:CHEBI:62555; Evidence={ECO:0000256|ARBA:ARBA00035855};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:31081;
CC Evidence={ECO:0000256|ARBA:ARBA00035855};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O;
CC Xref=Rhea:RHEA:26518, ChEBI:CHEBI:15377, ChEBI:CHEBI:57367,
CC ChEBI:CHEBI:58528; Evidence={ECO:0000256|ARBA:ARBA00036137};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:26520;
CC Evidence={ECO:0000256|ARBA:ARBA00036137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:58856; EC=4.2.1.17;
CC Evidence={ECO:0000256|ARBA:ARBA00035854};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:16107;
CC Evidence={ECO:0000256|ARBA:ARBA00035854};
CC -!- SUBUNIT: Homohexamer; dimer of trimers.
CC {ECO:0000256|ARBA:ARBA00038629}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000256|ARBA:ARBA00005254, ECO:0000256|RuleBase:RU003707}.
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DR RefSeq; XP_005190041.1; XM_005189984.3.
DR STRING; 7370.A0A1I8NEK3; -.
DR EnsemblMetazoa; MDOA014399-RA; MDOA014399-PA; MDOA014399.
DR GeneID; 101889661; -.
DR KEGG; mde:101889661; -.
DR VEuPathDB; VectorBase:MDOA014399; -.
DR eggNOG; KOG1680; Eukaryota.
DR OrthoDB; 553487at2759; -.
DR Proteomes; UP000095301; Unassembled WGS sequence.
DR Proteomes; UP000694905; Unplaced.
DR GO; GO:0016829; F:lyase activity; IEA:UniProt.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.12.10; Lyase 2-enoyl-coa Hydratase, Chain A, domain 2; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR PANTHER; PTHR11941:SF54; ENOYL-COA HYDRATASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11941; ENOYL-COA HYDRATASE-RELATED; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000694905}.
SQ SEQUENCE 294 AA; 31818 MW; 2A987CDC928968F9 CRC64;
MAQIMKTVAG RAQQMLQVAA KQQQFVPRFY SAAATNFEFI KTELAGEKKN VAVITLNRPK
ALNALCNGLM KELSTSLDQY EXDNSIAAIV ITGSEKAFAA GADIKEMQPN TYSKCIMGNF
LNDWTRVAKC QKPIIAAVNG YALGGGCELA MMCDIIYAGD KAKFGQPEIA LGTIPGAGGT
QRLTRVVGKS KAMEMCLTGN MISAAEAEKT GLVSKVVPAD QLVAEAVKLG EKIGTHSQLI
VQLCKEAVNT AYETTLQEGL KFERRTFHAT FSTEDRKEGM TAFVEKRPAK FNNN
//
