ID   A0A1I8QF11_STOCA        Unreviewed;       703 AA.
AC   A0A1I8QF11;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=Carboxylesterase type B domain-containing protein {ECO:0000259|Pfam:PF00135};
GN   Name=106096077 {ECO:0000313|EnsemblMetazoa:SCAU016533-PA};
OS   Stomoxys calcitrans (Stable fly) (Conops calcitrans).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC   Muscidae; Stomoxys.
OX   NCBI_TaxID=35570 {ECO:0000313|EnsemblMetazoa:SCAU016533-PA, ECO:0000313|Proteomes:UP000095300};
RN   [1] {ECO:0000313|EnsemblMetazoa:SCAU016533-PA}
RP   IDENTIFICATION.
RC   STRAIN=USDA {ECO:0000313|EnsemblMetazoa:SCAU016533-PA};
RG   EnsemblMetazoa;
RL   Submitted (MAY-2020) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetylcholine + H2O = acetate + choline + H(+);
CC         Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00000347};
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000256|ARBA:ARBA00005964}.
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DR   RefSeq; XP_013119080.1; XM_013263626.1.
DR   RefSeq; XP_013119081.1; XM_013263627.1.
DR   AlphaFoldDB; A0A1I8QF11; -.
DR   SMR; A0A1I8QF11; -.
DR   STRING; 35570.A0A1I8QF11; -.
DR   ESTHER; stoca-ACHE; ACHE.
DR   EnsemblMetazoa; SCAU016533-RA; SCAU016533-PA; SCAU016533.
DR   EnsemblMetazoa; SCAU016533-RB; SCAU016533-PB; SCAU016533.
DR   GeneID; 106096077; -.
DR   KEGG; scac:106096077; -.
DR   VEuPathDB; VectorBase:SCAU016533; -.
DR   OrthoDB; 4386at2759; -.
DR   Proteomes; UP000095300; Unassembled WGS sequence.
DR   GO; GO:0043083; C:synaptic cleft; IEA:GOC.
DR   GO; GO:0003990; F:acetylcholinesterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001507; P:acetylcholine catabolic process in synaptic cleft; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR001445; Acylcholinesterase_insect.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR000997; Cholinesterase.
DR   PANTHER; PTHR43918; ACETYLCHOLINESTERASE; 1.
DR   PANTHER; PTHR43918:SF13; ACETYLCHOLINESTERASE; 1.
DR   Pfam; PF00135; COesterase; 1.
DR   PRINTS; PR00880; ACHEINSECT.
DR   PRINTS; PR00878; CHOLNESTRASE.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Serine esterase {ECO:0000256|ARBA:ARBA00022487}.
FT   DOMAIN          96..651
FT                   /note="Carboxylesterase type B"
FT                   /evidence="ECO:0000259|Pfam:PF00135"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        326
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
FT   ACT_SITE        455
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
FT   ACT_SITE        568
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
SQ   SEQUENCE   703 AA;  77548 MW;  C52E9FF11C62B9EE CRC64;
     MARSIKASLS SSSSSQSQSP LSSSSSRLSQ SSSSLYSLKK STSSSSLTSS LSRPSSFGLV
     SYLLATRNND ICRGLLATLV ILLRMTALAS AMTDHLIVQT TSGPVRGRSV TVQGREVHVF
     TGIPYAKPPV DDLRFRKPVP AEPWHGVLDA TRLPATCVQE RYEYFPGFSG EEIWNPNTNV
     SEDCLFMNIW APAKARLRHG RGTNGGEHSA KTDQDHLIHS ATPQNTTNGL PILIWIYGGG
     FMTGSATLDI YNAEIMSAVG NVIVASFQYR VGAFGFLHLS PVMPGFEEEA PGNVGLWDQA
     LALRWLKENA RAFGGNPEWM TLFGESAGSS SVNAQLMSPV TRGLVKRGMM QSGTMNAPWS
     HMTSEKAVEI GKALINDCNC NASLLPDNPQ AVMACMRSVD AKTISVQQWN SYSGILSFPS
     APTIDGAFLP ADPMTLLKTA DLSGYDILIG NVKDEGTYFL LYDFIDYFDK DDATSLPRDK
     YLEIMNNIFN KATQAEREAI IFQYTSWEGN PGYQNQQQIG RAVGDHFFTC PTNEYAQSLA
     ERGAGVHYYY FTHRTSTSLW GEWMGVLHGD EIEYFFGQPL NTSLQYRPVE RELGKRMLNA
     VIEFAKTGNP AVDGEEWPNF SKEDPVYYVF STDEKTEKLQ RGPLAKRCSF WNDYLPKVRS
     WAGSECEGNI SLSASGTIYQ TQQQHSVLLS VALVLTLVFS IFQ
//