ID A0A1J0GFR6_9CLOT Unreviewed; 521 AA.
AC A0A1J0GFR6;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE SubName: Full=Arabinofuranosidase {ECO:0000313|EMBL:APC40147.1};
GN ORFNames=A7L45_08735 {ECO:0000313|EMBL:APC40147.1};
OS Clostridium estertheticum subsp. estertheticum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1552 {ECO:0000313|EMBL:APC40147.1, ECO:0000313|Proteomes:UP000182569};
RN [1] {ECO:0000313|Proteomes:UP000182569}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 8809 {ECO:0000313|Proteomes:UP000182569};
RX PubMed=27891116;
RA Yu Z., Gunn L., Brennan E., Reid R., Wall P.G., Gaora O.P., Hurley D.,
RA Bolton D., Fanning S.;
RT "Complete Genome Sequence of Clostridium estertheticum DSM 8809, a Microbe
RT Identified in Spoiled Vacuum Packed Beef.";
RL Front. Microbiol. 7:1764-1764(2016).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP015756; APC40147.1; -; Genomic_DNA.
DR RefSeq; WP_071612438.1; NZ_CP015756.1.
DR AlphaFoldDB; A0A1J0GFR6; -.
DR STRING; 1552.A7L45_08735; -.
DR KEGG; ceu:A7L45_08735; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000182569; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000182569}.
FT DOMAIN 319..512
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 183
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 121
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 521 AA; 58956 MW; D7DFC54BD9D58E35 CRC64;
MKTFINPILP GFYPDPSICR VKEDYYLVTS SFAYYPGVPI FHSRDLVNWK QIGHVLDRPS
QLPLDGLGQS RGIFAPTIRY NDGVFYMITT NMDGGGNFIV TATDPCGPWT NPYYLDAQGM
DPSLLFDDDG KVYYTGTRPT EEGPKYDGNW EIWLQELDLQ SMKLIGKPLG LWRGALREAI
WPEGPHIYKI NGEYYLMISE GGTGPQHAVS VARSNTICGP YVGNPGNPIL THRHLGKKYP
IVNVGHCDLV ETQNGEWWAV ALASRIYGGY YRNLGRETYL VPVIWEDGWP VMSAGTGKVE
LTYNMPNLPE NKVIVPSVCD DFDEDTLNFR WNFIRTPREE FFSLSERKGY LRLNLRPERI
TQRVNPSFVG RRQQHIKFSA STLMEFTPLS ENEVAGIVLL QSNDFNFSFI YTKNGDETVI
RLIKCTGGDE KIIVEKAVRG TTLYLKVEAD GQDYSFYYGE SLEKCIVLIE NVDGRILSTD
VAGGFVGTEI GLYASSNGIK SDNKAYFDWF KYYGEDGFNN D
//
