ID A0A1J0VQ93_9NOCA Unreviewed; 920 AA.
AC A0A1J0VQ93;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=BOX37_09680 {ECO:0000313|EMBL:APE34185.1};
OS Nocardia mangyaensis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=2213200 {ECO:0000313|EMBL:APE34185.1, ECO:0000313|Proteomes:UP000183810};
RN [1] {ECO:0000313|EMBL:APE34185.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Y48 {ECO:0000313|EMBL:APE34185.1};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; CP018082; APE34185.1; -; Genomic_DNA.
DR RefSeq; WP_071927365.1; NZ_CP018082.1.
DR AlphaFoldDB; A0A1J0VQ93; -.
DR KEGG; nsl:BOX37_09680; -.
DR Proteomes; UP000183810; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000183810};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 29..291
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 332..509
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 676..883
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 920 AA; 100196 MW; 9EE75DE45D91D1F0 CRC64;
MSGGVPRLTR VSSPTTAEAS TTTTPGEGER PTLLLLDGHS IAYRAFFALP AENFKTVTGQ
TTNAVYGFTS MLINLLRDEK PSHVAAAFDV SRKTFRTEAY PEYKANRSAT PDEFRGQVEL
TKDVLGAMGI PVMAIDGYEA DDVIATLTTQ AMAQGYRVLI VTGDRDAIQL VNDDVTVLYP
RKGVSDLTRF TPAEVETKYG LTPAQYPDYA ALRGDPSDNL PGIPGVGEKT AAKWIREYGD
LATLVDKVDQ VKGKVGDSLR ANLSSVVLNR QLTEMVREVP LPYAPDQLAK APWDRDKIHQ
LFDDLEFRVL RDRLFETLGS PEPEAESGFE ISGGALDQGA VADWLAEHAK AGVRHGLSIV
GTGTPMHGDA RALAIAAADG EGGYIDLAVL TPEDETALSA WLADPAVPKA LHEAKAAGHA
LRGRGFTLAG LTSDTALSAY LVRPGQRSFN LDDLSLRYLK RELRAETGDS GQLSLLDDED
TLAADIAREE MLRARAVADL ADALDAELAQ IESTALLHEM ELPLLGVLGE LEEAGIAVDV
AQLESLQRQF ADRVTEAANS AYEVIGKQIN LGSPKQLQVV LFDELDMPKT KRTKTGYTTD
ADALESLFEK TGHAFLEHLL AHRDATRLKV TVDGLLKSVS DDGRVHTTFN QTIAATGRLS
STEPNLQNIP IRTDTGRQIR DTFVVGPGFA ELMTADYSQI EMRIMAHLSG DEGLIEAFNS
GEDLHTFVAS KAFDIPLSEV SPELRRRIKA MSYGLAYGLS SYGLSAQLKI STAEAKEQME
VYFDRFGGIR DYLADVVDQA RKVGYTQTLF GRRRYLPDLD SSNRQRRESA ERMALNAPIQ
GTAADIIKVA MINVQNAIRE AGLRSRMLLQ IHDELVFEVA DGEREVLEAL ARDQMSEAIE
LSVPLDVSVG TGRSWDSAAH
//
