ID A0A1J1JM69_9CYAN Unreviewed; 3029 AA.
AC A0A1J1JM69;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Non-ribosomal peptide synthase, involved in Hassallidin biosynthesis {ECO:0000313|EMBL:CZT62785.1};
GN Name=hasN {ECO:0000313|EMBL:CZT62785.1};
GN ORFNames=PL8927_110038 {ECO:0000313|EMBL:CZT62785.1};
OS Planktothrix serta PCC 8927.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Oscillatoriales; Microcoleaceae; Planktothrix.
OX NCBI_TaxID=671068 {ECO:0000313|EMBL:CZT62785.1};
RN [1] {ECO:0000313|EMBL:CZT62785.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PCC 8927 {ECO:0000313|EMBL:CZT62785.1};
RA Ploux O.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CZT62785.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PCC 8927 {ECO:0000313|EMBL:CZT62785.1};
RA Gugger M., Pancrace C.;
RT "Hassallidin in the filamentous cyanobacterium Planktothrix serta PCC
RT 8927.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:VXD10550.1, ECO:0000313|Proteomes:UP000184550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BBR_PRJEB10992 {ECO:0000313|EMBL:VXD10550.1};
RG Genoscope - CEA;
RA William W.;
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; LT546031; CZT62785.1; -; Genomic_DNA.
DR EMBL; CZCU02000013; VXD10550.1; -; Genomic_DNA.
DR OrthoDB; 9778383at2; -.
DR Proteomes; UP000184550; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd17643; A_NRPS_Cytc1-like; 1.
DR CDD; cd19543; DCL_NRPS; 1.
DR CDD; cd19534; E_NRPS; 2.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 4.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 4.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR NCBIfam; TIGR01720; NRPS-para261; 2.
DR PANTHER; PTHR45398; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR PANTHER; PTHR45398:SF1; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 4.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 8.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT DOMAIN 973..1047
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2479..2553
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 3029 AA; 340613 MW; 885509472DA04C58 CRC64;
MEVYVLPLSL AQRQIWFNEI LSPGNIAYNI PIALKLVGDL HQEALKQSFH QIIYRHEVLR
TTFAIEDGEP VQLIHPEGGF ELEEKSLEFS LDDQPDQLKT ILERESQSPF NLVNDFLIRG
VLYRISNQDH ILLVNLHHII SDGWSLGILV QELAEFYRAY LQGSSVSLPE LPIQYGDYAE
WQVTWLKSQT IQEQITYWKK TLTTPLPNLE LLLDKKRPPL QTFHGTVWRQ VLPVSLTSSL
EAFAKANNAT FFMVTLAAYY VLLYRYTGQS DIIVGTVSAN RQHSEITTLI GCFVNALPLR
ADLSGRPTFR QLLQQISLTC VAALANQDVP LELIVEELEI QRNPQRSPIF QTLFALQNAP
LGEIQLPGII ATPIYIDNGG AKFDLSLMLE PSGSGWQVAL EYNTDLFTAP TAQRLLTHYQ
HLLTAVLIDP DVDINILPIL THSDRQELLT LGSGQETTQE QATNLVELFT HSAHKHANQI
AISTTDQEIT YQELHQQASQ LAAVLQQKGI GIETRVGIYL ERSTNLIIAL LAVLLSGGTY
VPLDPQYPSE RLRFIAEDSG ITLLLTTESL LSHLPIQDRK ILVIDQLEPT QEFTLTTQIL
PQQAAYIIYT SGSTGQPKGC VITHNNVVRL MRKTDDWFGF NEKDVWTLFH SFAFDFSVWE
IWGALLYGGK LVIVQYLESR SPEAFRNLLQ RQGVTILNQT PSAFRQLMRA DQAFPDKLGL
RVIIFGGEAL ELQSLKPWIE RYGDNYPRLI NMYGITETTV HVTYRQITAE DIDQNPGSVI
GLTIPDLSLH ILDDTLEPTP IGVRGEIYVG GMGLSRGYIN QPTLTATKFI PDPYSLEPGA
RLYRTGDLAR RLASGDIEYL GRSDLQVKIR GFRIELGEIE AALTAFPEVA EAIVIAHTVT
TEDKRLVAYI VTNRQMTAEQ LRTTLKEQLP DYMLPQAFMF LDTMPLTAQG KIDRQALPIP
DWHQSAARQS FLPPQTEAEK VICAVWTKVL EVDGIGVEDN FFDLGGDSIL ALKVVAEIRR
QGWLLTPKEI FQEQTVKRLG VVVERVHSLT IPARTAEGSV LLTPIQKWFF SLNVANPHHW
NQALLFEVHQ SLEPTTIATA IQTVAAHHDT FRLRFAKEEG IWQQFYGRND ANLFAFATLD
LSDQDDLMQN AAKSKAVEHL QKSLNLSDGP LAAATYFYLG SNRLPQLLVV IHHLIVDGVS
WRILLQDIKE AINGSQLAPV STSFQQWSEF LQNFANSRHL QAERQFWIDT VKTETATLPL
DFPNALMENL EGTVKRISRQ LTTEQTQIFL TRANKAYRTQ PQELLIAALG KTLNQITQKT
TFQIIMEGHG RDELSSDLDI TRTLGWFTTL YPFCLELSSQ DLTVKNLVRT VKEQVRSVPK
CGFGYGLLRY LNPETADDLA LESPSQISFN YLGQVRGESG QNRLLNLLNE DVELTRHPEG
NCPHIIDVMA IVVEGQLRVD WLYSSQLHQE ETVNQWAESF QQNLAEILAH CCEVSKSISE
YTPSDFPLVR ISQPNLDSLQ ERFPQLEDVY PLSPLQEGML FHTILNSEDA VYFEQVAGKI
TGKLDVEKLN SAWQIVIKRH PSLRSAFIWD GQKEPLQVVN KEVKFDIFQQ DWCDFSVAEQ
EGLREQYLLN DRQNGFVLTA APLMRFAVFR LDNFTWQWIW SHCHIILDGW SLPIIFKEVL
TVYQAACQQT LFSLPPVPPY RHYIQWLLEQ NPQSAGEFWQ EALDGISTST RIAWQLPNET
ESDTYAEVEL RLDAQQFAQL QQMAKSCRLT LNTIAQGAWG LCLQKHGAGE DVVFGVTVSG
RPSELQDVEN MVGLFINTLP MRIRLNPEVT VATWLEKIQQ HHTQMREYEY SKLIDIQKEI
SASAGEPLFD SILVFENYPV DKSLKEQGRD FQVHDIEFYE RTNYPLTLGV IPDDGLLLKL
NYQTKYLSAT AANILLGRLQ NIMVNLARYP DRVLGEITGF SAQDAVDMIN AGKAKNITSG
EFKAAHQLVE AQADLSPDAV ALVCDNQEVT YGELEERANQ IAAKLIEAGV GYETIVGLYF
HPSIAYIIAL LAVLKAGAAF LPLESSYPEA RLQFIVEDSK TPVILTSENL SPGLFSDKVK
VITFSKEPEK PTPHSTKRLN KKISSENLAY VIYTSGSTGK PKGVLVTHAG IQNLVKSQAL
SFGVTAESRV YQFASLNFDA AISEIFMALG SGATLYLQKN NNAVSSALWA NLTKWQVTHL
TLPPSLLAIV EPSELPELSS LIVAGEAATS EVFQRWGKRS ARVFNAYGPT EATVCASIMN
CSNLLGEPSI GKAIANVEIY LLDSFLQPVA PGVIGEIYIG GISLARGYLS RPELTGEAFI
PHPFATKPGA RLYRTGDRGT YDSQGNICFI GRKDSQIKLN GYRIELGEIE ATLIKHPTVD
SAVVMLRQDV PGRKRLVAYA CITKDSPATN ELRDYLVQKL PAYMVPSTIV PLKQWPLTPN
GKIDRTALPT PELTTFAATP KTATEEIFAQ IWVEVLGLET VHPQDNFFEL GGDSIMSLQV
VSRARTAGWE INPKDIFEAQ TLSRIAARAK PLSQQIEVTE PLTGVVPLSP IQHWFFAQNL
LHPHHWNQAV ALSIDEPLNI DAFSRTLDAI AFHHDVLRLK FSQNQGKWEQ SYSGAATRPA
LRVLDFSNLP EDALTAAVET EHESIQLEQS PLFRILYAKN LAKYGNVLFF LAHHLIVDGV
SWRILLADLS QAYQQAINDL PISLPLKTSS YRQWTTSLTT LANSKEIIED FPFWQNILST
PVAILPVDYP LLPNTNTVDS QAVRSCQLTT QETNTLVKVA IATYHASVQE IMLAALLKTL
YEVYQLEPWL IDLEGHGREE LHDQLDISRT VGWFTSIYPI LLKKPADSDQ YSLLKEIKTQ
LRTIPHHGIS FGLLHYLNQE KNLQQIANNQ KASISFNYLG QIDNLTKNHY PFKMSHAPTG
TGLFGKQERS HLLAINARIQ NEYLQVDWSY SQQIHHPQTI NRLAEVYLNN LRLYLTDSPA
TDSSFYSATD FQLVNLSESE LNSLLEDLE
//
