ID A0A1J4MCQ4_9CRYT Unreviewed; 246 AA.
AC A0A1J4MCQ4;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=tRNA (guanine(9)-N(1))-methyltransferase {ECO:0000256|ARBA:ARBA00012797};
DE EC=2.1.1.221 {ECO:0000256|ARBA:ARBA00012797};
GN ORFNames=cubi_01742 {ECO:0000313|EMBL:OII71267.1};
OS Cryptosporidium ubiquitum.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Cryptosporidiidae; Cryptosporidium.
OX NCBI_TaxID=857276 {ECO:0000313|EMBL:OII71267.1, ECO:0000313|Proteomes:UP000186176};
RN [1] {ECO:0000313|EMBL:OII71267.1, ECO:0000313|Proteomes:UP000186176}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=39726 {ECO:0000313|EMBL:OII71267.1};
RA Liu S., Roellig D.M., Guo Y., Li N., Frace M.A., Tang K., Zhang L.,
RA Feng Y., Xiao L.;
RT "Reductive evolution of mitochondrial metabolism and differential evolution
RT of invasion-related proteins in Cryptosporidium.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00000855};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OII71267.1}.
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DR EMBL; LRBP01000030; OII71267.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J4MCQ4; -.
DR VEuPathDB; CryptoDB:cubi_01742; -.
DR OrthoDB; 2908056at2759; -.
DR Proteomes; UP000186176; Unassembled WGS sequence.
DR GO; GO:0052905; F:tRNA (guanosine(9)-N1)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd18089; SPOUT_Trm10-like; 1.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; TRNA (GUANINE-9-) METHYLTRANSFERASE; 1.
DR PANTHER; PTHR13563:SF13; TRNA METHYLTRANSFERASE 10 HOMOLOG A; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 33..244
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000259|PROSITE:PS51675"
SQ SEQUENCE 246 AA; 28445 MW; A16F7689B62D098F CRC64;
MESETSLESI YDELKGRTKK RVNDNRKNRN FKCNQDYLKN IGIGPTIVID CDYDDKLSER
NLKSLVVQLS ISYSCIRKSK IPLKMIICGV SQRLKQGLYK TLAESWLGVE ILNDKVDQVI
SNRFLNTDSN YEIIYLSADS NNVLPYKKNQ DEYENSTDQG FNNKQIFIIG GIIDRNKYKN
ISKLRAEEFN IKTAKLPIIE SGITLCSNQV LTINHVIECI VKYNETCNWY TTFDSVLPKR
KKDQIN
//
