ID A0A1J4VFD5_9BACT Unreviewed; 539 AA.
AC A0A1J4VFD5;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=Glycoside hydrolase {ECO:0000313|EMBL:OIO32895.1};
GN ORFNames=AUJ70_04530 {ECO:0000313|EMBL:OIO32895.1};
OS Candidatus Omnitrophica bacterium CG1_02_40_15.
OC Bacteria; Candidatus Omnitrophota.
OX NCBI_TaxID=1805284 {ECO:0000313|EMBL:OIO32895.1, ECO:0000313|Proteomes:UP000183570};
RN [1] {ECO:0000313|EMBL:OIO32895.1, ECO:0000313|Proteomes:UP000183570}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CG1_02_40_15 {ECO:0000313|EMBL:OIO32895.1};
RX PubMed=27112493;
RA Probst A.J., Castelle C.J., Singh A., Brown C.T., Anantharaman K.,
RA Sharon I., Hug L.A., Burstein D., Emerson J.B., Thomas B.C., Banfield J.F.;
RT "Genomic resolution of a cold subsurface aquifer community provides
RT metabolic insights for novel microbes adapted to high CO concentrations.";
RL Environ. Microbiol. 0:0-0(2016).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIO32895.1}.
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DR EMBL; MNVP01000077; OIO32895.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J4VFD5; -.
DR STRING; 1805284.AUJ70_04530; -.
DR Proteomes; UP000183570; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Hydrolase {ECO:0000313|EMBL:OIO32895.1}.
FT DOMAIN 8..330
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 425..530
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT ACT_SITE 190
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 351
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 242
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 259
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 405
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 470
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
SQ SEQUENCE 539 AA; 62804 MW; 236AE42EC32A1D93 CRC64;
MHKGYLCLVL HAHLPFVRHP EEEDFLEENW LYEAITETYI PFIQVFEKLI EDHVDFRITM
SLTPTLASML QDELLQNRYI RHIDKLIELA DKEVQRTGYD PKFHGLALMY YRKFVEAKEI
FVDRCKKDLV SAFKRFQDIG CVEILASCAT HGFLPILSVN PTDADAQVKI GVGHYRKTFG
RDPVGFWLPE CGYAPGIDNL LADSGIKYFF VDSHGIVNAD PAPKYSVYAP LYCPTGVAAF
GRDWESSKQV WSSKEGYPGD PDYREYYKDI GHELEYEYIK PYIHPIGFRI NTGLKYWRIT
GDTDHKEPYV PEWAKEKAAT HAGNFMFNRE KQIEHVAYHM DRKPMIISPY DAELFGHWWF
EGPMWIDFLI RKIAYDQKTV KLATPSDYLR EYPVNQMSVP STSTWGYKGY NEVWLEGSND
WIYRHLHAAG ERMKELAEKF SNCIEKDKFG LAALALNQAA RELLLAQASD WPFIMKTGTM
VEYANKRVKL HINRFTHIYK DMLNGSIDKE WLKEVESRDN IFSDMDCAKY YSSRLSLCV
//
