ID A0A1J4VQ35_9BACT Unreviewed; 889 AA.
AC A0A1J4VQ35;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=AUJ71_01150 {ECO:0000313|EMBL:OIO39242.1};
OS Candidatus Omnitrophica bacterium CG1_02_49_16.
OC Bacteria; Candidatus Omnitrophota.
OX NCBI_TaxID=1805290 {ECO:0000313|EMBL:OIO39242.1, ECO:0000313|Proteomes:UP000182296};
RN [1] {ECO:0000313|EMBL:OIO39242.1, ECO:0000313|Proteomes:UP000182296}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CG1_02_49_16 {ECO:0000313|EMBL:OIO39242.1};
RX PubMed=27112493;
RA Probst A.J., Castelle C.J., Singh A., Brown C.T., Anantharaman K.,
RA Sharon I., Hug L.A., Burstein D., Emerson J.B., Thomas B.C., Banfield J.F.;
RT "Genomic resolution of a cold subsurface aquifer community provides
RT metabolic insights for novel microbes adapted to high CO concentrations.";
RL Environ. Microbiol. 0:0-0(2016).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIO39242.1}.
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DR EMBL; MNVU01000021; OIO39242.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J4VQ35; -.
DR STRING; 1805290.AUJ71_01150; -.
DR Proteomes; UP000182296; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 1..257
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 295..476
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 643..850
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 889 AA; 100203 MW; 1FC52A579148C0A0 CRC64;
MKVILLDGNS FCYRAFYAIR ELRNSKGQAT NAVYGFIVML EKLLKDVNPD GIAVTFDMKG
PTFRHKRYDQ YKIQRLPMPD DLVSQMPIIK EVVSAMNIPI FQKEGFEADD VIATLAKKLE
AAGHEPIIVT ADKDALQLVN KKISVLNPQK ENQVYDEEAV KKRFGVGPKQ VVEIMALMGD
ASDNIPGVPG IGEKTASKLI IEFGTLEGVL KNLSKIKGEK IKENLKTYEA EARLSKELAQ
VDCQVPLLLT IEKLKRQEPD AEKLVRLYQT LEFKTLIKNL SAAEQKNSED PSLEYYLIED
KKSLAALKEK LSKKSEWAFD FETTGTNALT AEPIGISFSF KEKEAFYVNF NKTTLKAEQC
LGFMKDLFQD KNIKKIGQNL KYEVLILKNF GIALDGIAFD TMVASYCLNP AKPNHNLDDI
TMEHLGLRIT SITELIGTGQ NQIPMESLPI DQVYRYGCQD SDITFRLSKI LAKKLEEKDL
IELFQEIEMP LVSVLADMEH AGIAVDKKLL SELSEEMEKE LKSLTLKIHK EAGKEFNINS
PKQLAEILFE TLGLPVVKKT KTGMSTDVEV LQELAEIHSL PKEILKFREL SKLKSTYVDA
LPLLVNSKTQ RVHTSFNQTV TATGRLSSSD PNMQNIPVRT REGRKIRGAF VAGVRTNSLV
SADYSQIELR VLAHLSGDEN LIRAFQEGAD IHRYTASLIF NVELTGVTEA MRDSAKTVNF
GVLYGMGAFS LAKSLHITND AARDFIKAYF DRVPRVKKYL DETLEKARQD GFVSTYFKRR
RYIPEILSKD IRMKKFAERT AINMPIQGTA SDIIKIAMRE ISARLVKEKF KALMILQVHD
ELLFEVPNDE LKDLIALVTQ EMQGAVLFKV PIKVSVKAGP NWLDMVEVE
//
