ID A0A1J4X2Y2_9BACT Unreviewed; 505 AA.
AC A0A1J4X2Y2;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Methionine--tRNA ligase {ECO:0000256|ARBA:ARBA00018753};
DE EC=6.1.1.10 {ECO:0000256|ARBA:ARBA00012838};
DE AltName: Full=Methionyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030904};
GN ORFNames=AUJ11_00260 {ECO:0000313|EMBL:OIO52839.1};
OS Parcubacteria group bacterium CG1_02_44_65.
OC Bacteria.
OX NCBI_TaxID=1805311 {ECO:0000313|EMBL:OIO52839.1, ECO:0000313|Proteomes:UP000182601};
RN [1] {ECO:0000313|EMBL:OIO52839.1, ECO:0000313|Proteomes:UP000182601}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CG1_02_44_65 {ECO:0000313|EMBL:OIO52839.1};
RX PubMed=27112493;
RA Probst A.J., Castelle C.J., Singh A., Brown C.T., Anantharaman K.,
RA Sharon I., Hug L.A., Burstein D., Emerson J.B., Thomas B.C., Banfield J.F.;
RT "Genomic resolution of a cold subsurface aquifer community provides
RT metabolic insights for novel microbes adapted to high CO concentrations.";
RL Environ. Microbiol. 0:0-0(2016).
CC -!- FUNCTION: Is required not only for elongation of protein synthesis but
CC also for the initiation of all mRNA translation through initiator
CC tRNA(fMet) aminoacylation. {ECO:0000256|ARBA:ARBA00003314}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|RuleBase:RU363039}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIO52839.1}.
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DR EMBL; MNWL01000008; OIO52839.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J4X2Y2; -.
DR Proteomes; UP000182601; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 2.170.220.10; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00398; metG; 1.
DR PANTHER; PTHR43326:SF1; METHIONINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43326; METHIONYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039}.
FT DOMAIN 3..80
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 138..359
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 393..480
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 505 AA; 58207 MW; BE856D8F3EFE8E7D CRC64;
MAKKFYITTT APYVNADPHI GFALEIIQAD AVARYHRLLG EEVIFGCGTD EHGLKIYRKA
LEEKLSPQKY CDQSVTAWNK LKQVLNLSYT HFIRTTERRH LEAAQALWQR CQDNGDIYKK
TYKSKYCVGC ELEKTDSELE QGRCPIHPNL EIELINEENY FFRFSKYQKP LLDFYAQNPD
FVVPRYRFNE IIEFVKNGLE DFSVSRLKSK MPWGVDIPGD PNHVMYVWFD ALVFYISTLG
WPKIADFAGF WPGIQVAGKD NLRQQSAIWQ AMLMSASLPN TRQILIHGFI QSGGQKMSKS
LGNVIDPIFL SQKYGADALR YYLLAKIHPF NDSDFTIQLF EEAYNSDLAN GLGNLTSRII
TMCQKFTGGV VPPKIELKNH PLRKLEIAVW KMLEQKMPTF EFHLVLESIW NFIHALDKYI
DETKPWVLAK QGKQKQIDEI IFVLLDCLKD LAWLIAAFLP EAAQKITTAL NLKDLLAKDP
IYKDNWASLK PGTKIKPIKS LFPRI
//
