ID A0A1J5FX13_9BACT Unreviewed; 695 AA.
AC A0A1J5FX13;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=ATP-dependent DNA helicase RecG {ECO:0000256|ARBA:ARBA00017846, ECO:0000256|RuleBase:RU363016};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU363016};
GN Name=recG {ECO:0000256|RuleBase:RU363016};
GN ORFNames=AUK19_01925 {ECO:0000313|EMBL:OIP59544.1};
OS Candidatus Moranbacteria bacterium CG2_30_45_14.
OC Bacteria; Candidatus Moranbacteria.
OX NCBI_TaxID=1805255 {ECO:0000313|EMBL:OIP59544.1, ECO:0000313|Proteomes:UP000183500};
RN [1] {ECO:0000313|EMBL:OIP59544.1, ECO:0000313|Proteomes:UP000183500}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CG2_30_45_14 {ECO:0000313|EMBL:OIP59544.1};
RX PubMed=27112493;
RA Probst A.J., Castelle C.J., Singh A., Brown C.T., Anantharaman K.,
RA Sharon I., Hug L.A., Burstein D., Emerson J.B., Thomas B.C., Banfield J.F.;
RT "Genomic resolution of a cold subsurface aquifer community provides
RT metabolic insights for novel microbes adapted to high CO concentrations.";
RL Environ. Microbiol. 0:0-0(2016).
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA). {ECO:0000256|RuleBase:RU363016}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363016};
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000256|ARBA:ARBA00007504, ECO:0000256|RuleBase:RU363016}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIP59544.1}.
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DR EMBL; MNYT01000026; OIP59544.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J5FX13; -.
DR STRING; 1805255.AUK19_01925; -.
DR Proteomes; UP000183500; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd04488; RecG_wedge_OBF; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR NCBIfam; TIGR00643; recG; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_dom3_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363016};
KW DNA damage {ECO:0000256|RuleBase:RU363016};
KW DNA recombination {ECO:0000256|RuleBase:RU363016};
KW DNA repair {ECO:0000256|RuleBase:RU363016};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU363016};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363016};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363016}.
FT DOMAIN 273..448
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 468..629
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 695 AA; 78451 MW; C68F620520F468AE CRC64;
MFTFDTPLSN IPTIKKQTLG KLERLGLFTV CDMLYHFPSR YEDYSKLRSI DTLLLDEKCT
IEGKIIYIEA GRTWKRKMFL TEAILEDASG QIRLIWFNQR FVTQSLKQGM IIRASGKITK
DSRGLTLTNP AFENATRNAT HTGRLVPIYP ETAGLTSRFF RWQLTGIFQK LTTFPDPISK
EVLTRLHLPS LKQALYYIHF PKNEDFSLLA HKRFAFDEML FIQLKALQIK TLFETSEGSP
ISYDETTLKK FIASFPFVLT DAQKKAYQEI LIDLGKTRPM NRLLNGDVGS GKTIVAAIGA
FVTAERGFQT AILAPTEVLA RQHFENLSRI FEETDHSLAL FTGSYRILDS KSVTRPTLIK
AISAGIPKII IGTHALLQDD VTFDAIALIV VDEQHRFGVS QRARLQELSF NNLDGSKEKV
PHFLSMTATP IPRTLTLAFF GNLDLSLLDG LPKDRKPIIT KIARTSVDRE KVYTFVQSEI
ASGRQVFIIF PLVEESLALK DIKAAVAQHK HLAETVFPHL SIGLVHGKMK AKEKEMVMQD
FHEKKYDILV ATAVVEVGID VPNASIILIE EAERFGLSQL HQFRGRVGRA EHQSYCFLFP
HEQANSINER LRILEKTTNG FEIAEADLGI RGPGAFFGTR QSGIPDIAME SLTNIKLITI
ARKEAKNILA KDTDLSSHPL LKDALKKFKE KIHLE
//
