ID A0A1J5I919_9BACT Unreviewed; 536 AA.
AC A0A1J5I919;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=B12-binding domain-containing protein {ECO:0000259|PROSITE:PS51332};
GN ORFNames=AUK05_00650 {ECO:0000313|EMBL:OIP87720.1};
OS Candidatus Shapirobacteria bacterium CG2_30_35_20.
OC Bacteria; Candidatus Shapirobacteria.
OX NCBI_TaxID=1805376 {ECO:0000313|EMBL:OIP87720.1, ECO:0000313|Proteomes:UP000182344};
RN [1] {ECO:0000313|EMBL:OIP87720.1, ECO:0000313|Proteomes:UP000182344}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CG2_30_35_20 {ECO:0000313|EMBL:OIP87720.1};
RX PubMed=27112493;
RA Probst A.J., Castelle C.J., Singh A., Brown C.T., Anantharaman K.,
RA Sharon I., Hug L.A., Burstein D., Emerson J.B., Thomas B.C., Banfield J.F.;
RT "Genomic resolution of a cold subsurface aquifer community provides
RT metabolic insights for novel microbes adapted to high CO concentrations.";
RL Environ. Microbiol. 0:0-0(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIP87720.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MNZO01000009; OIP87720.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J5I919; -.
DR STRING; 1805376.AUK05_00650; -.
DR Proteomes; UP000182344; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 26..165
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
SQ SEQUENCE 536 AA; 62258 MW; 4E45C8D71858E291 CRC64;
MTFLIFLTMP LNTSKNFKTS PKLPKPSVIL YFPTPFPYHR PWKGVPLALL AISRILDKER
YTVKIYAPFL QNNSIKEIIK YTKQNKVVCL GVSAMTGFQI YDGLKISKQF KKVFSKIPII
WGGWHPSILP IQTVKNKFID IVVRGQGDKT FPRLVHALEK KQNLKSINGL TYKYKNKIIT
TPDSTPVKLD DMPPLPYHLI NIDKCLWGTE YGVKTLPYIS SYGCPHRCGF CVEEVVNKRH
WTALSAQNIF KEWKTLIKKY KVDSIAVYDS NFFVNEKRVS DLCKLLVKYK LNIKWGNANG
RVRQLSQFKP ETWKLMKKTG CAMILTGAES GNQKALDLIS KDMNAEEIIT FTKLCHKYKI
KILYSFLVGL PWSKNSSENK IFVADEYKST LSLIDKLLKI STTNRYTYYV FLPYPGAPMF
HRAVSFGYIA PKTLVGWSNY LMSPEDAFQS VLRQKWITPK QATMTAMLTQ YIFGLMDLDS
INMLSQRINS KIGKFIFKFT YKITLIIVKL RWKYKYFDLP LDYWLFTQVH KYARLI
//
