ID A0A1J5WM97_9MICR Unreviewed; 419 AA.
AC A0A1J5WM97;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=rRNA m(5)C methyltransferase RCM1 {ECO:0000313|EMBL:OIR57912.1};
GN ORFNames=A8A55_1324 {ECO:0000313|EMBL:OIR57912.1};
OS Amphiamblys sp. WSBS2006.
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Amphiacanthidae;
OC Amphiamblys.
OX NCBI_TaxID=1866961 {ECO:0000313|EMBL:OIR57912.1, ECO:0000313|Proteomes:UP000182028};
RN [1] {ECO:0000313|EMBL:OIR57912.1, ECO:0000313|Proteomes:UP000182028}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSBS2006 {ECO:0000313|EMBL:OIR57912.1};
RX PubMed=27694476;
RA Mikhailov K.V., Simdyanov T.G., Aleoshin V.V.;
RT "Genomic survey of a hyperparasitic microsporidian Amphiamblys sp.
RT (Metchnikovellidae).";
RL Genome Biol. Evol. 0:0-0(2016).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIR57912.1}.
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DR EMBL; MKPU01000051; OIR57912.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J5WM97; -.
DR VEuPathDB; MicrosporidiaDB:A8A55_1324; -.
DR Proteomes; UP000182028; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:UniProt.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR049561; NSUN5_7_fdxn-like.
DR InterPro; IPR048889; NSUN5_RCM1_N.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF4; 28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF21148; NSUN5_fdxn-like; 1.
DR Pfam; PF21153; NSUN5_N; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000182028};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 122..417
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 352
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 232..238
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 256
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 283
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 303
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 419 AA; 46910 MW; C9BC278FBA29E067 CRC64;
MEGAKEGTGN IYEKAGHILK QVEKGKGKTL NVCLKTKMLR KDKALLKCVY GIVQETLKHS
KTISEVLDKT KIHAQEETDI DVLRTALSDF LYKKRLRCAG PLKEKITLHK EELRKAFREA
SSSDQTVSSL PTYLRVNTLR TTRKEAIARL QESGYCFIDT GDETGQAAQK NRNKKTFYID
KDIPNLLVFP SRVDLSENEL YKTGHLVFQQ KSSCFPALAL GVSGKATVID ACAAPGNKTS
HIAAMMENKG RIFAVEKDKN RAKLLKKMMA VAGVQNTVVC ETDFLSLSPS EQPYKDAKYI
LLDPSCSGSG MKGGLLLKKK QTQISNMAAT QKKLLLHALS FPSAEKIVYS TCSVNEEENE
EVVRAVLRER KDFALARALP LWHRRGLKKY SFADLVCRAD SDEDRTTGFF VAVFEKRNR
//
