ID A0A1J6IMX5_NICAT Unreviewed; 492 AA.
AC A0A1J6IMX5;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=A4A49_29433 {ECO:0000313|EMBL:OIT00211.1};
OS Nicotiana attenuata (Coyote tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=49451 {ECO:0000313|EMBL:OIT00211.1, ECO:0000313|Proteomes:UP000187609};
RN [1] {ECO:0000313|EMBL:OIT00211.1, ECO:0000313|Proteomes:UP000187609}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. UT {ECO:0000313|Proteomes:UP000187609};
RC TISSUE=Leaves {ECO:0000313|EMBL:OIT00211.1};
RA Xu S., Brockmoeller T., Gaquerel E., Navarro A., Kuhl H., Gase K., Ling Z.,
RA Zhou W., Kreitzer C., Stanke M., Tang H., Lyons E., Pandey P., Pandey S.P.,
RA Timmermann B., Baldwin I.T.;
RT "The genome of Nicotiana attenuata.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIT00211.1}.
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DR EMBL; MJEQ01037189; OIT00211.1; -; Genomic_DNA.
DR RefSeq; XP_019250958.1; XM_019395413.1.
DR AlphaFoldDB; A0A1J6IMX5; -.
DR EnsemblPlants; OIT00211; OIT00211; A4A49_29433.
DR GeneID; 109229863; -.
DR Gramene; OIT00211; OIT00211; A4A49_29433.
DR KEGG; nau:109229863; -.
DR OMA; SSAWESC; -.
DR OrthoDB; 5474774at2759; -.
DR Proteomes; UP000187609; Chromosome 7.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18037; DEXSc_Pif1_like; 1.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR47642; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR47642:SF5; ATP-DEPENDENT DNA HELICASE; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000187609}.
FT DOMAIN 311..349
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 492 AA; 54750 MW; 4F461AD81962F2D4 CRC64;
MKLSLYTATT AYRFLSSKAA CWSQASAKSK VKLTNPKKPR VKLTDQQNQI LEAISNGNSV
FITGSAGTGK TYLLQDIITK LRKIHGKSRV FVTASTGVAA CSLNGQTLHS FAGIGLGDAS
DVDLLSRVTL DKRAYRRWNK VRALVIDEIS MISGEVFDNL EFIARSIRSD ELGCEDKIWG
GIQLVVSGDF FQLPPVINKK GHNKEFAFEA ECWNASFDMQ IELKTIFRQS DAQLIKLLQG
IRKGKYDSDD LQLLDRCCST VEPDASAVQL YPRIEDVSRV NADRLDCLDE VLFHYQALDS
GKDPWKKQLK NGIAPELLKL CVGARVLLTK NIDVIGGLVN GATGTILDFA VVQDTHKLYD
HEISDICLNG NLLPVVKFDS GQELMIGLEK WYVMDGDEAV AMRKQIPLIL AWALSIHKCQ
GMTLNNLHTD LYRVFGFGMV YVALSRVKSL DGLHLVNFNP SKIKAHPKVL QFYQILSGEK
DELKEDAVSD EI
//
