ID A0A1J6JKF7_NICAT Unreviewed; 507 AA.
AC A0A1J6JKF7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=O-acyltransferase wsd1 {ECO:0000313|EMBL:OIT07417.1};
GN Name=WSD1_12 {ECO:0000313|EMBL:OIT07417.1};
GN ORFNames=A4A49_34834 {ECO:0000313|EMBL:OIT07417.1};
OS Nicotiana attenuata (Coyote tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=49451 {ECO:0000313|EMBL:OIT07417.1, ECO:0000313|Proteomes:UP000187609};
RN [1] {ECO:0000313|EMBL:OIT07417.1, ECO:0000313|Proteomes:UP000187609}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. UT {ECO:0000313|Proteomes:UP000187609};
RC TISSUE=Leaves {ECO:0000313|EMBL:OIT07417.1};
RA Xu S., Brockmoeller T., Gaquerel E., Navarro A., Kuhl H., Gase K., Ling Z.,
RA Zhou W., Kreitzer C., Stanke M., Tang H., Lyons E., Pandey P., Pandey S.P.,
RA Timmermann B., Baldwin I.T.;
RT "The genome of Nicotiana attenuata.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIT07417.1}.
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DR EMBL; MJEQ01037183; OIT07417.1; -; Genomic_DNA.
DR RefSeq; XP_019228582.1; XM_019373037.1.
DR AlphaFoldDB; A0A1J6JKF7; -.
DR STRING; 49451.A0A1J6JKF7; -.
DR EnsemblPlants; OIT07417; OIT07417; A4A49_34834.
DR GeneID; 109209693; -.
DR Gramene; OIT07417; OIT07417; A4A49_34834.
DR KEGG; nau:109209693; -.
DR OMA; HIICDEM; -.
DR OrthoDB; 340468at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000187609; Chromosome 1.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR PANTHER; PTHR31650:SF28; O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:OIT07417.1};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Reference proteome {ECO:0000313|Proteomes:UP000187609};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OIT07417.1}.
FT DOMAIN 36..297
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 358..487
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 507 AA; 57168 MW; 6F9C070AB34AA188 CRC64;
MERDENKMVL MEPVRTTKKL DENEEEPEAL LMSPTSRMFH EPNYNVYTLA IMGWKIPLNV
DAMKAEIQGK LLKHPRFSSL QAVDESDDSR KMKWVPTTVN IDDHITVPDL KSIPNNMDSH
LADKLVEEYI SNLSTTTIDM SKPLWDLHIL NVKTSHADAT CIFRFHHSLG DGVSLISLLL
SCFRKTSDPT SLPTLLVSSS SSSKGKSNLS NNRRSIRSLI WQCLVKLWWC IRLLFNTIID
VLLFVATALF LKDSQSPFTV PQGFKLSTRL RFIYRTISLD DIKFIKNSTN STVNDVILGI
TQAALSRYIH RRYEIEGKRK FSPQKMRCRA NVVVNLRPAL GVQTVAEMIE KNAIVIQGNC
FGFVIIPLSI SQLDNPLDYV LKAKTTMDRK KHSLESRCTF YFSQLLIKLF GFEGATTLAA
RVLSQTTLIF SNVAGPPEEV SCSGHPLTFL APTCYGQPTG LMVHGCSYAK KLTFAVAVDE
GIIPDPNLES FRLIKEAALS KWRTKVD
//
