ID A0A1J6X2T2_9BACI Unreviewed; 577 AA.
AC A0A1J6X2T2;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=B12-binding domain-containing radical SAM protein {ECO:0000313|EMBL:OIU72441.1};
GN ORFNames=BHE18_07390 {ECO:0000313|EMBL:OIU72441.1};
OS Rossellomorea aquimaris.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Rossellomorea.
OX NCBI_TaxID=189382 {ECO:0000313|EMBL:OIU72441.1, ECO:0000313|Proteomes:UP000182062};
RN [1] {ECO:0000313|EMBL:OIU72441.1, ECO:0000313|Proteomes:UP000182062}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SAMM {ECO:0000313|EMBL:OIU72441.1,
RC ECO:0000313|Proteomes:UP000182062};
RA Waghmode S.R., Suryavanshi M.V.;
RT "Bacillus aquimaris SAMM genome sequence reveals colonization and
RT biosurfactant production capacities.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIU72441.1}.
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DR EMBL; MINN01000074; OIU72441.1; -; Genomic_DNA.
DR RefSeq; WP_071618089.1; NZ_MINN01000074.1.
DR AlphaFoldDB; A0A1J6X2T2; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000182062; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000182062}.
FT DOMAIN 2..135
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 174..414
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 577 AA; 67836 MW; 7A0D9371C7EE2838 CRC64;
MKKVVLSTLN AKYIHTNLAI RCLKAYIQPH YDAELAEYTI KDPVLNIATD LFSKKPDVIG
FSCYIWNIEE TITVIQVLKK ILPEVKIVLG GPEVTYDVPY WLDRLEGEVD FIVLGEGEET
FKQLLDEIHG DENWSNVHGI AYLDNSKPVI KPQQNKIDLR NVPSPFRFEE DLPQLSKRVT
YIETSRGCPF RCQFCLSSIE VGVRYFDREK VKEDIRFLME HGAKTIKFVD RTFNISRSYA
MEMFQFLIDE HLPGTVFQFE ITADIMRPEV IEFLNQEAPP GLFRFEIGVQ STNDETNDLV
MRKQNYKKLT RTVTMVKDGG KIDQHLDLIA GLPEEDYHSF RKTFNDVFEL RPEELQLGFL
KMLRGTGLRI RANDHQYIYM DHSPYEILGN NVLSFDDILR IKQVEDVLEK YWNDHRMDQT
IEHLVTNCFE TPFDFFQQFG SYWESRGWSR IGHQLEDLYK RLYEFLSTQG KHDMRVIEGL
MKLDYLSNQK YKPRKPWWEI EMTKEDRSSV YQELLKSPQI AGEEFMILNL TEKEIYKHTL
LETIVSPDGG NQYVLAYYEP ADGRSSLFLF DRQCIPH
//
