ID A0A1J7C5A6_9GAMM Unreviewed; 318 AA.
AC A0A1J7C5A6;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=BK025_13960 {ECO:0000313|EMBL:OIV46135.1};
OS Sodalis sp. TME1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Bruguierivoracaceae; Sodalis.
OX NCBI_TaxID=1912097 {ECO:0000313|EMBL:OIV46135.1, ECO:0000313|Proteomes:UP000181913};
RN [1] {ECO:0000313|EMBL:OIV46135.1, ECO:0000313|Proteomes:UP000181913}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TME1 {ECO:0000313|EMBL:OIV46135.1,
RC ECO:0000313|Proteomes:UP000181913};
RA Rosas-Perez T., Vera-Ponce De Leon A., Rosenblueth M., Ramirez-Puebla S.T.,
RA Rincon-Rosales R., Martinez-Romero J., Dunn M.F., Martinez-Romero E.;
RT "The Symbiome of Llaveia Cochineals (Hemiptera: Coccoidea: Monophlebidae)
RT Includes a Gammaproteobacterial Co-symbiont Sodalis TME1 and the Known
RT Candidatus Walczuchella monophlebidarum.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA.
CC {ECO:0000256|ARBA:ARBA00002876}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC Evidence={ECO:0000256|ARBA:ARBA00000381};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIV46135.1}.
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DR EMBL; MNBX01000442; OIV46135.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1J7C5A6; -.
DR Proteomes; UP000181913; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF93; PSEUDOURIDYLATE SYNTHASE RPUSD4, MITOCHONDRIAL; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000181913};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 20..79
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 144
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 318 AA; 35505 MW; 1715B9D2E6D8C453 CRC64;
MNENNPGVHF ITITADEAGQ RIDNFLRTHL KGVPKSMIYR IVRKGEVRVN KKRVKPEYKL
QNDDEVRIPP VRRSDDEQAP VSTGLDKVAA LSDAVLYEDD YLLILNKPAG TAVHGGSGLS
FGVIEGLRAL RPDARFLELV HRLDRDTSGV LLVAKKRSAL RALHEQLRAK GMQKDYLALV
RGQWQSHVKS VAAPLLKNIL ASGERIVRVN SEGKPSETRF KVEERFTLAT LVRASPITGR
THQIRVHAQH AGHPIAFDDR YGDGAFDQQL TGCGLNRLFL HAAALRFDHP GSGETLRIEA
PLDAPLRQCL LYLRAHHK
//
