ID A0A1J7CE38_9ACTN Unreviewed; 418 AA.
AC A0A1J7CE38;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=6-phospho-beta-glucosidase {ECO:0000313|EMBL:OIV37938.1};
GN ORFNames=BIV57_08350 {ECO:0000313|EMBL:OIV37938.1};
OS Mangrovactinospora gilvigrisea.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Mangrovactinospora.
OX NCBI_TaxID=1428644 {ECO:0000313|EMBL:OIV37938.1, ECO:0000313|Proteomes:UP000243342};
RN [1] {ECO:0000313|EMBL:OIV37938.1, ECO:0000313|Proteomes:UP000243342}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MUSC 26 {ECO:0000313|EMBL:OIV37938.1,
RC ECO:0000313|Proteomes:UP000243342};
RA Lee L.-H., Ser H.-L.;
RT "Genome sequence of Streptomyces gilvigriseus MUSC 26.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIV37938.1}.
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DR EMBL; MLCF01000040; OIV37938.1; -; Genomic_DNA.
DR RefSeq; WP_071656083.1; NZ_MLCF01000040.1.
DR AlphaFoldDB; A0A1J7CE38; -.
DR STRING; 1428644.BIV57_08350; -.
DR OrthoDB; 9767022at2; -.
DR Proteomes; UP000243342; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd05296; GH4_P_beta_glucosidase; 1.
DR Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092:SF5; 6-PHOSPHO-BETA-GLUCOSIDASE; 1.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000243342}.
FT DOMAIN 191..394
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT BINDING 91
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 145
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 165
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT BINDING 195
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT SITE 107
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ SEQUENCE 418 AA; 45306 MW; EA6D3AA8A36455CF CRC64;
MTKLTVVGGG STYTPELIDG FARLRDTLPV DELVLMDPDA ERLRLVGGLA RRIFAHQGHP
GTITTTQDLD AAVDGADAVL LQLRVGGQAA RAQDEAWPLE CGCVGQETTG AGGLAKALRT
VPVVLDIAER VRRANPDAWI VDFTNPVGIV TRALLTAGHR AVGLCNVAIG VQRNVAGLLG
VEPKRIALDH VGLNHLTWVR GVRLDGEQIA DRLLDEHADR IAEDLHLDAG YIRRHRVVPS
YYLRYYYEHD AVVREMRGAE SRAQKVAAIE RELLEMYADP ALTEKPKLLE QRGGAYYSEA
AVALVASLLG DSGDEQVVNV RNDGTLPFLP DDAVVEVPAR ITAGGPVPLP QAPVEPLHSG
LIAHVTGYEY LALDAALRGG RERVYDALLA HPLVGQFELA EQLTDRLIAH NEQHLGWA
//
