ID A0A1J7GY81_LUPAN Unreviewed; 538 AA.
AC A0A1J7GY81;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 08-NOV-2023, entry version 30.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=TanjilG_10884 {ECO:0000313|EMBL:OIV95064.1};
OS Lupinus angustifolius (Narrow-leaved blue lupine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC genistoids sensu lato; core genistoids; Genisteae; Lupinus.
OX NCBI_TaxID=3871 {ECO:0000313|EMBL:OIV95064.1, ECO:0000313|Proteomes:UP000188354};
RN [1] {ECO:0000313|EMBL:OIV95064.1, ECO:0000313|Proteomes:UP000188354}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Tanjil {ECO:0000313|Proteomes:UP000188354};
RC TISSUE=Whole plant {ECO:0000313|EMBL:OIV95064.1};
RX PubMed=27557478; DOI=10.1111/pbi.12615;
RA Hane J.K., Ming Y., Kamphuis L.G., Nelson M.N., Garg G., Atkins C.A.,
RA Bayer P.E., Bravo A., Bringans S., Cannon S., Edwards D., Foley R.,
RA Gao L.L., Harrison M.J., Huang W., Hurgobin B., Li S., Liu C.W.,
RA McGrath A., Morahan G., Murray J., Weller J., Jian J., Singh K.B.;
RT "A comprehensive draft genome sequence for lupin (Lupinus angustifolius),
RT an emerging health food: insights into plant-microbe interactions and
RT legume evolution.";
RL Plant Biotechnol. J. 15:318-330(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
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DR EMBL; CM007376; OIV95064.1; -; Genomic_DNA.
DR RefSeq; XP_019419244.1; XM_019563699.1.
DR AlphaFoldDB; A0A1J7GY81; -.
DR STRING; 3871.A0A1J7GY81; -.
DR EnsemblPlants; OIV95064; OIV95064; TanjilG_10884.
DR GeneID; 109329842; -.
DR Gramene; OIV95064; OIV95064; TanjilG_10884.
DR KEGG; lang:109329842; -.
DR OMA; DMEASKC; -.
DR OrthoDB; 930987at2759; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000188354; Chromosome LG16.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF73; 3-KETOACYL-COA SYNTHASE 1; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000188354};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 59..80
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 100..118
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 116..404
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 423..503
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 260
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 339
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 424
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 428
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 457
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 461
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 538 AA; 60426 MW; CA106BDE67FA0615 CRC64;
MNGGSSIGMD KERLTAEMDF KDSTSAVIKI RRRLPDFLHS VKLKYVKLGY GYSCNTTTIF
FTLIFPLFIT LLFQFTGLNL NLHHLTHFFS GSNRTVQLDS YTLACSLLGI FLFGLYYAKR
SRPVYLVDFS CYKPEKDQKI SVDGFLKMTE ELGAFEEETL QFQRRVSSKA GLGDETYLPS
GITSRPPKLN LQEARIEAEA VMFGALDSLF AKTGVQPRNI DILVVNCSLF NPTPSLSAMI
VNHYKMRSDI KSYNLGGMGC SAGLISIDLA KDLLKANPNS YVVVVSTENI TLNWYFGNDR
SMLLSNCIFR MGGAAILLSN KGSDRVRSKY ELVHTVRTHK GADDKNFNCV YQKEDESGKI
GVCLARELMA VAGDALKTNI TTLGPLVLPF SEQFMFFMSL MRKKIMKGSR VKPYIPDFKL
AFEHFCIHAG GRAVLDELQK NLELSEWHME PSRMTLHRFG NTSSSSLWYE LAYTEAKGRV
NKGDRVWQIA FGSGFKCNSA VWKAVKAMPA VKDWRGNPWD DSVDKYPVHV PTSIGNTS
//
