ID   A0A1J9QAM6_9EURO        Unreviewed;       555 AA.
AC   A0A1J9QAM6;
DT   15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT   15-FEB-2017, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   RecName: Full=Amidase domain-containing protein {ECO:0000259|Pfam:PF01425};
GN   ORFNames=AJ78_07134 {ECO:0000313|EMBL:OJD12229.1};
OS   Emergomyces pasteurianus Ep9510.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Ajellomycetaceae; Emergomyces.
OX   NCBI_TaxID=1447872 {ECO:0000313|EMBL:OJD12229.1, ECO:0000313|Proteomes:UP000182235};
RN   [1] {ECO:0000313|EMBL:OJD12229.1, ECO:0000313|Proteomes:UP000182235}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UAMH 9510 {ECO:0000313|EMBL:OJD12229.1,
RC   ECO:0000313|Proteomes:UP000182235};
RG   The Broad Institute Genomics Platform;
RA   Cuomo C.A., Munoz J.F., Imamovic A., Priest M.E., Young S., Clay O.K.,
RA   McEwen J.G.;
RT   "Emmonsia species relationships and genome sequence.";
RL   Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OJD12229.1}.
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DR   EMBL; LGRN01000431; OJD12229.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1J9QAM6; -.
DR   STRING; 1447872.A0A1J9QAM6; -.
DR   VEuPathDB; FungiDB:AJ78_07134; -.
DR   OrthoDB; 731186at2759; -.
DR   Proteomes; UP000182235; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1300.10; Amidase signature (AS) domain; 1.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR46072:SF11; AMIDASE-RELATED; 1.
DR   PANTHER; PTHR46072; AMIDASE-RELATED-RELATED; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   PIRSF; PIRSF001221; Amidase_fungi; 1.
DR   SUPFAM; SSF75304; Amidase signature (AS) enzymes; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000182235}.
FT   DOMAIN          76..539
FT                   /note="Amidase"
FT                   /evidence="ECO:0000259|Pfam:PF01425"
FT   ACT_SITE        132
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        207
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        231
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   BINDING         181
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT   BINDING         207
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT   BINDING         228..231
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
SQ   SEQUENCE   555 AA;  60828 MW;  CFDD59A893E83BB8 CRC64;
     MGQTYKEISE VARKRRDDAL SAFLPIPDIA EDGLPRDLRS YPKTSGLLTT EELDIVDTDA
     ETLLQKIKDR KLTSVEVAKA FCKASVIAQK LTNCVTEVLF NEGLERARYL DEYLEKTGSV
     IGPLHGLPLS LKDSLIIPPH PSSIGMAVHA NVPTQQDSVL VRMLRALGAV FYVKTNIPTA
     MMMAETTNRI WGETRNPIHK GLTPGGSTGG EGALLAMKAS PLGVGTDIAG SIRIPSAFCL
     LYGLKPSFGR FSTLGGQPSI AGQDFIYAVC GPMSPSLGAV KLFCESVLSD SAAPWNLDPK
     IIPMPWRKDV IQPKGRKLRF GVLGSNDGTI TCHPPIERAL STVTNALKAA GHDVIPWEPI
     NHQEIVDLLN ESFDELGSDA IIPVLEEYQE PIFGSMKRAF SACKNESRKF DPAKLRQMII
     KRNQFQTEYL ERWMATKTDT DGPIDGIIAP VAPTGAPRLG LGETVEYVGF TGFVNLLDYS
     ACTFPVTYAD KSIDLPRGEG WVPLNPMDKM IQADYDPEFY HRAPVSLQLI GKRLEEEKVI
     EMVEVVADVL KKSQV
//