ID A0A1K0FSQ7_9ACTN Unreviewed; 5124 AA.
AC A0A1K0FSQ7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:OJF15837.1};
GN ORFNames=BG844_02330 {ECO:0000313|EMBL:OJF15837.1};
OS Couchioplanes caeruleus subsp. caeruleus.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Couchioplanes.
OX NCBI_TaxID=56427 {ECO:0000313|EMBL:OJF15837.1, ECO:0000313|Proteomes:UP000182486};
RN [1] {ECO:0000313|EMBL:OJF15837.1, ECO:0000313|Proteomes:UP000182486}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 43634 {ECO:0000313|EMBL:OJF15837.1,
RC ECO:0000313|Proteomes:UP000182486};
RA Sheehan J., Caffrey P.;
RT "Couchioplanes caeruleus draft genome sequence.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OJF15837.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MEIA01000010; OJF15837.1; -; Genomic_DNA.
DR RefSeq; WP_071803044.1; NZ_MEIA01000010.1.
DR Proteomes; UP000182486; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd19543; DCL_NRPS; 2.
DR CDD; cd19540; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 3.40.50.980; -; 8.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 6.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 6.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 4.
DR NCBIfam; TIGR01720; NRPS-para261; 1.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF13193; AMP-binding_C; 4.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00823; PKS_PP; 4.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 4.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 12.
DR PROSITE; PS00455; AMP_BINDING; 4.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000182486}.
FT DOMAIN 957..1031
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2019..2093
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3554..3629
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4607..4682
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 4894..4913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5124 AA; 548843 MW; 621D5BCD91F36FD2 CRC64;
MFGGTGAGAP LTRAQTGIWL AQQIDPENAV YNIARYLEFR GDVDVDVDRL AAAVRQVVDK
AQCLHVRFVV GLDGEPRQIG GEPVEWSLPV VDLSAEPDPD AAAAAWMRAD REQVVHLARG
PLFAHALLRL AADRVLWYAR YHHLVMDGYG GALIARRVGE LYTTGVVDST REAWSVDRLV
DSDAAYRVSE RYQTDRDYWH RRFADRPEPA RLLTRATEPV GRLERRSVRL GPDQAGRLHK
TAADAGTRVS RVAIAAVAVY LHRATGAQDI VLALPVTARV DAALRNVPGM VSNVLPLRLT
VRPETTPAEL LASVATEVAG LTAHSRYRGE ELARDLGLPD GIAELAGPSV NIFAYEHGMR
LGDLEVSGNT LWQGPVTDLS ILVEPQSDGR CLRVDLEADA AVCSPAELAE HERRFLAVLD
AIVTHPDRPV GTIDLLRGEE RLVLERFGTA EREVPEVTWP AAVEAQAARS PHAVAVVCES
EQLSYAGLNA AANRLARVLR DRGVRPEDVV AIALPRSTEL VVALLAVMKA GAAYLPLDAD
HPADRIGYML ADAGARLVVS TAELAAPFAD LPGDCLLLDE AGAELAAADD RNLELAIGLH
QAAYVIYTSG STGAPKGVVI SHEGIGSLIA TAVDRLGVDA DSRVVQFASV GFDVAVWDLC
MSLCVGGRVI VVPTERRVAG APLTDYVTEH GATHMILPPS LVAALPPGCA LPDGAVLVVG
TETVPTELIA RWAQRLRVVA AYGLTEATVN STLWAAKPDW RGPVPIGVPD PNTRCYVLDS
ALRPVGVGVE GELYVGGRGL ARGYRGRPGL TAERFVADPY GAPGSRMYRT GDRARWRADG
NLDFLGRADH QIKIRGYRIE PGEVASVLMR HASVAQAAVV AAEDHRGVKR LVAYVTPVDG
DVDPATLRAH AAAVLPEYMV PSVIVGVDGP LPLTPNGKLD TAALPAPDWT ALAGDAAPST
PAEHTLAALF AEVLRLPAVG VHDSFFSLGG DSIVAIALVN LARTAGLAIT PRDVFRHRTV
AALAAAAGPA QTGPVVADSP AAFSLVALDP AERTEFAALV PDLDDVLPAT PLQEGFYFHA
SYDDAAGDVY AVQQTIDLLG DVDADAVRRS VQGLLDRHPL LRAGFRQRSG GQLVQLIAGR
VDVPWRAVDL TGTAPAERPA KAAHVAAQER ANRFDLARPP LLRAALVRLD ADRHRLVLTV
HHIVVDGWSV SIMMRELLDG VRGELPAGRD ALHRDYFGWL GSRDLDAARA AWRTALAELD
GPTRLLPAEV GGAAPKPEQL RVELPATTTA GLTARARERG LTASTVIHGA WGLLLGRLVG
REDVVFGSTV SGRHADVDGV QSLVGLLINT LPVRLRWRAE EPLADVLARF QDEQSALMEH
QHLGLADIQR LVGWGELFDT LVVVENLPSA GDLRDETGVE LAGVEVRDAT HYPVALTVLP
GERMELRLDH DPDRIDAAAA DRLAAALVRL LETVIDDPDR PVGRLDLASD ADRDAMASEL
AGAHHEVPAI TLAAAVTARA RRNPDATAVI SADERLSYAD LDAAADAVAR RLRERGAAPE
RIVAVAVPRS ARLPVALLGV MKTGAAYLPL DLDHPAERIA FMLADSGART VVTTPEASAL
LPRLDGVEPL ILDGAEPAGG VTTAAFDADP AGPEHPAYLL YTSGSTGRPK GVLVPHRAVV
SQLTWACEQF GYRADDRVLH QYSATFDPSV LEIFAPLLAG AAVVIAKPDG HRDPAYLAEL
IRRERVTTVD LVPSMYAALL QATEGSGADG WAAGLRRAFS GGEALGGGLA RRWRDRTGVP
MINVYGPTEA TVQVTSWEYD GAVDAGVPIG LPAWNTRLYV LDPYLRPVPA GASGELYIAG
SQLARGYHGR SGLTAERFVA DPFGAPGERM YRTGDLVRWR TDGAVEYLGR SDHQVKIRGN
RVELGEIENR LAALPQVATA VVAVRTDDRG GAQLVGYVVP SAGARPDGEA LRAALAGALP
EPMVPAAFVV LDSLPLTPGG KVDRAALPAP TVVRAVAQRP ADERERLLCD IFAAVLGLAE
VGADEDFFVL GGDSILSISV SSRARAGGLN LSPREVFLHR TPAALAVAAA DRPRAEAAAP
VADGVGDVPL LPIAHQLRCD GGPVGRFNLS VLVQTPHDAD AALLSAVLQA VLDHHDGLRQ
RLTRVAGVLW SLEALPAGVV TAAELLRRVD VADLDAAELR AVLAAESDAA ADRLDPDSGV
MLQAVWFDAG SGRPGRLLLA THHLVVDGVS WRILLGDLAA AWEAVHAGRT PVLEPVPTSL
RRFARLVGEQ AQAPQRLAEL PHWLTTLAPG ADLVAGAPGP GTVGESRARQ VRLPIADTLP
LLTSVPAAVN GDVTDVLLTA LRVAVSRWRD RRGEDGAAEL LVDVERHGRE EIAEGLDLSR
TVGWFTSVQP VRLAAGPRPV DALKGVKERL RAAPDGGLGF GMLRHLNAQV APVLAQAASA
QVLFHYFGRF AADHGTDWTP APESDALTLA PDPGLGMPYL LQIDAVCADT PAGPELSAVW
TWRDVGLSET DVDELSEEWL AALRELAAAV DGAQRTAGLT PSDLTTIQLS QADIDRVEQL
SPSPVADIWP LSPLQEGIFF HSSYDQGALD VYTAQDTYDF GHRLELDRLR AACATLLTRH
PSLRAGLTSD GLHQPVQFIA AAVEVPLDEV DLSGLPAEEQ DRQMAELLAA DRGRRFDLAR
PPLCRLTVVR LAGDRDRLVL SHHLVAWDGW SQGLFLEELL ELYATGGDDT ALATPGTYRD
YLAWLAGQDA DRTVDVWRAA LAGLAEPTLV SPADRSREPV IPRRCRTELP AELSDRLRSE
SRRHGLTLNT LLNAAWGLCL ASLVGRYDVV FGSTVAGRPA DVPRAESAIG MFLNTVPVRI
AMDPHESVLQ LLRRLQSERT ALMNHEYVGL GEIQRASGHQ QLFDTLYVLQ NFGDVDSLTA
LRERHGITDI GSADATHYPM TLVVTPARDV RVALNYRPDV VEPASAESLL ERFTLLLSRM
IADLSAPVGT LDLLTASERT QLAAEWDATR RPMVSDTVAD LLAAQAARTP DALALVCGDQ
RLSYAELDGR INRLARLLLA RGAAPERVVA LALPRSVEMV VALFAVLRTG AAYLPLDLDH
PVDRLEMMIE DTGPMCVLST GAVARSLTTP AVCVDDPAVL AELAALSAAD VDDEERPGFA
RSRPDRLEHP AYVIYTSGST GRPKGVVTPY RGLTNMQLNH QEAIFGPAIA AAGGRRLRIA
HTVSFAFDMS WEELLWLVEG HEVHVCDEEL RRDATALVAY CETHRIDVVN VTPTYAHLLI
EEGLLSGHVP PLVLLGGEAV SDAVWTRLRD TEGTYGYNLY GPTEYTINTL GASTEDSATP
TVGRAIWNTR AYILDAHLRP VPPGTPGELY IAGIGLARGY HRRPGLTAER FVADPYGRPG
DRMYRTGDLA RSRPDGILDF LGRTDDQVKI RGYRVELGEI ATVLESHPEV SQAAVVADGA
GPGGARRLLG YVVAAGEATD ALPPELREYL KGRLPDYMVP AALTAVERLP LTVNGKLDVR
ALPAPEMRGG DSRPPASAEE ELLCALFAEV LGVPQVGVED SFFDLGGHSL LATRLVSRAR
AALGTELAIR DLFEAPTVAE LVLRAAAARG AVRPALVAGE RPDELPLSFA QQRLWVIQQL
EGASAAYNFP LVMRLRGALD ADAFAAALGD VVARHEALRT LFGEREGVPF QRVLPAAAAR
PPVELITAAQ DEVTAAVRAA VDRPFDLAAE LPLRATLVQV SEVEHVVVIL LHHITTDEWS
DRPFLRDLAA AYAARLGDTA PTFPPLPVQY ADYALWQQRL LGEREDPAGL AARQLDHWQR
ALDALPEELE LPFDRPRPAR PTFRGGELEL ELKPEVCERL RRLTQRTGAS MFMVLHAATA
ALLHRLGAGD DIPLGAPIAG RTDAGLDELV GFFVNTLVLR TNLSGDPSFA ELVARARETD
LAAFSHADVP FEAVVERVNP ARSLARNPLF QVMVGYHNRS DDALQLPGLE VEWVPVELPT
AKFDLVFSFS EHVESGRIGC RLEYATDLFD PGTAARLGDR LHRLLAGVTA DPQAPLSSID
LLAADERRQV VERYNDTLRA VPEETLPQLF ARCVAARPDA VAVVDRSRSA SYAQLDAQAN
RIARLLAARG VGRESVVGIA VPRSVEMVAT VLGVLKLGAA YLPLDLSHPA ERIAYMLGDS
AAGVVVATAS EAPRVPEVDG VRRLLLDSPP VAGELAALDP SPLPTGPVGL DQAAYVIYTS
GSTGRPKGVA VPHDGIASLA ATAVDRMSLT PDSRVLQFAS VGFDVAVFEL TMALCVGGQL
VLTPDEVRVA GPALTEFLHE RRITHMILPP SLVAALPADC ELPAGSTILV GTETVPPDLI
GRWAGRLNLL AAYGLTEATV NSTLWPAKPD WNASVPIGVP DPNTTVYVLD ARLRPVPPGV
VGELYVSGRG LARGYLGKPG LTAERFVACP FGPPGTRMYR TGDRARWRTD GNLDFLGRVD
EQVKIRGFRI ELGEISGALS GHPRVRQAAV VADRDGEHTR LIGYAVTGDD PVPPADLRAY
LAESLPDYMV PAAVVVLADP LPLTPNGKLD RRALPRPDWS TMTGDERPVT AEQHTLAELF
AEILGLPAVG IHDNFFALGG HSMAAMRLIG RIRSAFGADL TIREVFDTPT VAGLAGQLAG
ATSARPPLAA TERPAELPIA PVQRWRWDLH RDRPGYDHAL ALRSTGGLDA AALAAAVRDV
VTRHEPLRSL LVERDGAVYQ QLVDAPSLET ERVAGEGLDR RLAELAHEPV DLATAPPLRA
RLLADEAGAQ ALLFTMGYTG VDEWSVVPLF RDLSTAYAAR LAGNAPDWAL LPVSYADYTR
WQHDLLGDPD DPGSRHARQL NYWRQALRGV PAELALPTDR RRTGGPGHSG DYVEAPLDAD
LHRAVDRLAR ATGTSMFMVL QAALAALLTG RGAGTDLPIG TLVAGRTEES LVDLVGCFFN
TVVLRTDTTG DPTFAELLTR VRRTNLSALD RQDVPFADVL PELGGVRAPQ VMIVHHEQAD
LAGLDGGHGT MQAVPIRATA AELTLSFYEP RGDGAVQCYL EYATDLFDRP TVQRLADDLL
GLLAAAAADP DRALSTLSIS RSES
//
