ID   A0A1K1LTH5_RUMFL        Unreviewed;       360 AA.
AC   A0A1K1LTH5;
DT   15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT   15-FEB-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   RecName: Full=aspartate carbamoyltransferase {ECO:0000256|ARBA:ARBA00013008};
DE            EC=2.1.3.2 {ECO:0000256|ARBA:ARBA00013008};
GN   ORFNames=SAMN02910280_0744 {ECO:0000313|EMBL:SFW14229.1};
OS   Ruminococcus flavefaciens.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Ruminococcus.
OX   NCBI_TaxID=1265 {ECO:0000313|EMBL:SFW14229.1, ECO:0000313|Proteomes:UP000183461};
RN   [1] {ECO:0000313|EMBL:SFW14229.1, ECO:0000313|Proteomes:UP000183461}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YL228 {ECO:0000313|EMBL:SFW14229.1,
RC   ECO:0000313|Proteomes:UP000183461};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-
CC         aspartate + phosphate; Xref=Rhea:RHEA:20013, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29991, ChEBI:CHEBI:32814, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:58228; EC=2.1.3.2;
CC         Evidence={ECO:0000256|ARBA:ARBA00001363};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       (S)-dihydroorotate from bicarbonate: step 2/3.
CC       {ECO:0000256|ARBA:ARBA00004852}.
CC   -!- SIMILARITY: Belongs to the aspartate/ornithine carbamoyltransferase
CC       superfamily. {ECO:0000256|RuleBase:RU003634}.
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DR   EMBL; FPIP01000001; SFW14229.1; -; Genomic_DNA.
DR   RefSeq; WP_072299132.1; NZ_FPIP01000001.1.
DR   AlphaFoldDB; A0A1K1LTH5; -.
DR   UniPathway; UPA00070; UER00116.
DR   Proteomes; UP000183461; Unassembled WGS sequence.
DR   GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR   GO; GO:0004070; F:aspartate carbamoyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   Gene3D; 2.30.30.20; Aspartate carbamoyltransferase regulatory subunit, C-terminal domain; 1.
DR   Gene3D; 3.40.50.1370; Aspartate/ornithine carbamoyltransferase; 2.
DR   InterPro; IPR006132; Asp/Orn_carbamoyltranf_P-bd.
DR   InterPro; IPR006130; Asp/Orn_carbamoylTrfase.
DR   InterPro; IPR036901; Asp/Orn_carbamoylTrfase_sf.
DR   InterPro; IPR002082; Asp_carbamoyltransf.
DR   InterPro; IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
DR   InterPro; IPR020542; Asp_carbamoyltrfase_reg_C.
DR   InterPro; IPR036792; Asp_carbatrfase_reg_C_sf.
DR   NCBIfam; TIGR00670; asp_carb_tr; 1.
DR   PANTHER; PTHR45753:SF6; ASPARTATE CARBAMOYLTRANSFERASE; 1.
DR   PANTHER; PTHR45753; ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL; 1.
DR   Pfam; PF00185; OTCace; 1.
DR   Pfam; PF02729; OTCace_N; 1.
DR   Pfam; PF02748; PyrI_C; 1.
DR   PRINTS; PR00100; AOTCASE.
DR   PRINTS; PR00101; ATCASE.
DR   SUPFAM; SSF57825; Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain; 1.
DR   SUPFAM; SSF53671; Aspartate/ornithine carbamoyltransferase; 1.
DR   PROSITE; PS00097; CARBAMOYLTRANSFERASE; 1.
PE   3: Inferred from homology;
KW   Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003634}.
FT   DOMAIN          6..145
FT                   /note="Aspartate/ornithine carbamoyltransferase carbamoyl-P
FT                   binding"
FT                   /evidence="ECO:0000259|Pfam:PF02729"
FT   DOMAIN          153..302
FT                   /note="Aspartate/ornithine carbamoyltransferase Asp/Orn-
FT                   binding"
FT                   /evidence="ECO:0000259|Pfam:PF00185"
FT   DOMAIN          321..356
FT                   /note="Aspartate carbamoyltransferase regulatory subunit C-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF02748"
SQ   SEQUENCE   360 AA;  40367 MW;  711E2B53363C5EED CRC64;
     MISEYQHLID LNDYPVSWWK KVVELGEKIY QSPAEYAHVC DGKIMATLFY EPSTRTQMSF
     QTAMIRLGGQ IIGFDNPANS SVAKGETIKD TTKIVSNYAD VLVIRHPIAG AAKAASLTAD
     CSVINAGDGG HLHPTQTLTD LLTLKIEKKT LSGLTIGMCG DLLNGRTVHS LCKALSMFEN
     NKFVFISTSQ LGMPAYIKDI IKANGSSFVE VNTLEEVIGE LDVLYMTRIQ QERFASEEEY
     LAQKNTYVLD RKKMLGAKKD MIVMHPLPRV DEITVEVDDD ERAMYFKQAK YGMYVRMALI
     MTILSDKKSS ETLKGKVFSG VRCSNPKCIT NHEEYLPKSF RSSGDETLLE CEYCDERKLI
//