ID A0A1K1M132_9FIRM Unreviewed; 556 AA.
AC A0A1K1M132;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=SAMN02910447_00588 {ECO:0000313|EMBL:SFW16861.1};
OS Ruminococcus sp. YE71.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=244362 {ECO:0000313|EMBL:SFW16861.1, ECO:0000313|Proteomes:UP000182164};
RN [1] {ECO:0000313|EMBL:SFW16861.1, ECO:0000313|Proteomes:UP000182164}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YE71 {ECO:0000313|EMBL:SFW16861.1,
RC ECO:0000313|Proteomes:UP000182164};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; FPIR01000002; SFW16861.1; -; Genomic_DNA.
DR RefSeq; WP_072416711.1; NZ_FPIR01000002.1.
DR AlphaFoldDB; A0A1K1M132; -.
DR STRING; 244362.SAMN02910447_00588; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000182164; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:SFW16861.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000182164}.
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 556 AA; 59385 MW; A178B9E9C5624434 CRC64;
MLTDIEIAQQ AKMLRIGEIA KSLGIAEEDF EPYGHYKAKL SEQLYVKTAD KPDGKLILVT
AINPTPAGEG KTTVSVGLAE GMAKTGRNAI LALREPSLGP VFGIKGGAAG GGYAQVVPMD
DINLHFTGDM HAITAANNLL CALIDNHIHQ GNVLRIDQRR ILFKRCMDMN DRALRSVIVG
LGGKVNGFPR EDGFQITVAS EIMAILCLAA DLADLKARLE RILVGYDLDG NPVYAGQLQG
CGAMAALLKD ALKPNLVQTL ENTPAIMHGG PFANIAHGCN SVRATKLCLK LADYTITEAG
FGSDLGAEKF FDIKCRYAGL KPNCVVLVAT IRALKYNGGV ARADLKTENV EALKKGSVNL
RTHIENMQKF GVPVVVAINR FADDTDAEIA VIEEVCSQCG VEFSLTEVFA KGGEGAADLA
DKVVNAIETK PSQYKPLYDT ALPVKEKIGT IAREIYRADG VIYTAQAEKA LKEIEALGKT
DLPICVAKTQ YSLSDDPTKL GKPEGFEITV RDMKLSSGAG FIVVYTGDIM TMPGLPKAPA
ANNIDVDNAG NISGLF
//
