ID A0A1K1NDA2_RUMFL Unreviewed; 582 AA.
AC A0A1K1NDA2;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE SubName: Full=NAD(P)-dependent iron-only hydrogenase catalytic subunit {ECO:0000313|EMBL:SFW33426.1};
GN ORFNames=SAMN02910280_1878 {ECO:0000313|EMBL:SFW33426.1};
OS Ruminococcus flavefaciens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=1265 {ECO:0000313|EMBL:SFW33426.1, ECO:0000313|Proteomes:UP000183461};
RN [1] {ECO:0000313|EMBL:SFW33426.1, ECO:0000313|Proteomes:UP000183461}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YL228 {ECO:0000313|EMBL:SFW33426.1,
RC ECO:0000313|Proteomes:UP000183461};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; FPIP01000004; SFW33426.1; -; Genomic_DNA.
DR RefSeq; WP_072300155.1; NZ_FPIP01000004.1.
DR AlphaFoldDB; A0A1K1NDA2; -.
DR Proteomes; UP000183461; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR CDD; cd00207; fer2; 1.
DR Gene3D; 3.10.20.740; -; 1.
DR Gene3D; 3.30.70.20; -; 1.
DR Gene3D; 3.40.50.1780; -; 1.
DR Gene3D; 4.10.260.20; Iron hydrogenase, small subunit; 1.
DR InterPro; IPR036010; 2Fe-2S_ferredoxin-like_sf.
DR InterPro; IPR001041; 2Fe-2S_ferredoxin-type.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR009016; Fe_hydrogenase.
DR InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR InterPro; IPR003149; Fe_hydrogenase_ssu.
DR InterPro; IPR036991; Fe_hydrogenase_ssu_sf.
DR InterPro; IPR013352; Fe_hydrogenase_subset.
DR InterPro; IPR000283; NADH_UbQ_OxRdtase_75kDa_su_CS.
DR InterPro; IPR019574; NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
DR NCBIfam; TIGR02512; FeFe_hydrog_A; 1.
DR NCBIfam; NF040763; FeFe_hydrog_A6; 1.
DR PANTHER; PTHR11615:SF345; CATALYTIC SUBUNIT OF IRON-ONLY HYDROGENASE-RELATED; 1.
DR PANTHER; PTHR11615; NITRATE, FORMATE, IRON DEHYDROGENASE; 1.
DR Pfam; PF02906; Fe_hyd_lg_C; 1.
DR Pfam; PF02256; Fe_hyd_SSU; 1.
DR Pfam; PF13510; Fer2_4; 1.
DR Pfam; PF12838; Fer4_7; 1.
DR Pfam; PF10588; NADH-G_4Fe-4S_3; 1.
DR SMART; SM00902; Fe_hyd_SSU; 1.
DR SMART; SM00929; NADH-G_4Fe-4S_3; 1.
DR SUPFAM; SSF54292; 2Fe-2S ferredoxin-like; 1.
DR SUPFAM; SSF54862; 4Fe-4S ferredoxins; 1.
DR SUPFAM; SSF53920; Fe-only hydrogenase; 1.
DR PROSITE; PS51085; 2FE2S_FER_2; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
DR PROSITE; PS51839; 4FE4S_HC3; 1.
DR PROSITE; PS00641; COMPLEX1_75K_1; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 2..82
FT /note="2Fe-2S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51085"
FT DOMAIN 82..121
FT /note="4Fe-4S His(Cys)3-ligated-type"
FT /evidence="ECO:0000259|PROSITE:PS51839"
FT DOMAIN 142..172
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51379"
FT DOMAIN 185..214
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51379"
SQ SEQUENCE 582 AA; 63406 MW; 94B1A788C3A7C58E CRC64;
MENITVKING VELEVPKGTT VLEAAHMAGF EIPTLCYMKE INEIGACRIC VTEVNEGRGF
RLVAGCVYPC SNNMEILTNS PKVIESRKRT LELILSTHDR KCLSCVRSGN CELQKLAKDY
GVEDATVYDG VKNEYEVDNS APHMYRDNNK CILCRRCVAV CAKTQGIGVI GANERGFKTY
IGSAFDMGLG ETSCVSCGQC IAVCPVGALS EKDYTKEVMA AIADPEKTVL VQTAPAVRAG
LGECFGNPIG TNAEGKMVAA LRRLGFDKVF DTDFAADLTI MEEANEFLDR FKNGGTLPLI
TSCSPGWVKF CEHYYPDLLD HLSTCKSPQQ MFGATAKTYY AQKMGIDPKN IVMVSIMPCT
AKKFEIGRPD QSAAGVPDVD YALTTRELGR MIERAGILFN SLPEEKFDDP LGISTGAGVI
FGATGGVMEA ALRTAVYTLT GDNVTDLPEV RGTKDIKEAT YNVAGTDVNV IVTSGLANAR
KVLDSIRDGS CKAQFIEIMA CPGGCVNGGG QPQVPMGVRN FVDIREERAK VLYNLDKSMP
LRQSHENPAI KKVYEEFFEK PGSHKAHEVL HTSYIKRKVN EI
//
