ID A0A1K1Q3Q8_9FIRM Unreviewed; 786 AA.
AC A0A1K1Q3Q8;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=CRISPR-associated endonuclease/helicase Cas3 {ECO:0000313|EMBL:SFW54544.1};
GN ORFNames=SAMN02910447_03624 {ECO:0000313|EMBL:SFW54544.1};
OS Ruminococcus sp. YE71.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=244362 {ECO:0000313|EMBL:SFW54544.1, ECO:0000313|Proteomes:UP000182164};
RN [1] {ECO:0000313|EMBL:SFW54544.1, ECO:0000313|Proteomes:UP000182164}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YE71 {ECO:0000313|EMBL:SFW54544.1,
RC ECO:0000313|Proteomes:UP000182164};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
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DR EMBL; FPIR01000038; SFW54544.1; -; Genomic_DNA.
DR RefSeq; WP_072419571.1; NZ_FPIR01000038.1.
DR AlphaFoldDB; A0A1K1Q3Q8; -.
DR STRING; 244362.SAMN02910447_03624; -.
DR Proteomes; UP000182164; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR006674; HD_domain.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR Pfam; PF01966; HD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Endonuclease {ECO:0000313|EMBL:SFW54544.1};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:SFW54544.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000182164}.
FT DOMAIN 9..195
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 257..440
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 786 AA; 88881 MW; FDD4BBD523C0900E CRC64;
MFTAHISADG TRRQSVFEHS MNTARLAEGY LEAVGLSSSG KLAGLLHDGG KLTRRFDDYI
NGISDARRGE IDHSYTGAKY LFEEILTDKG GKNKAPATLI AHTIISHHAL HDWIDEHDDD
YFIKRISKTD CYKEASANIK EMLLETDLYK LFEKASDEIW IIIGKLKTAD KNEMAFYIGM
LERLIESALI DADRTDTADF TSSRNTAFAS DNSSLWTEMN ERMESKMNSF ESIQNSLPPR
EKSISERRRS ISDRCADFAG HKAGACRLIV PTGGGKTLSS LRFAIKQCQK FGMDRIFYVA
PFMSILEQNS DIIRSIAGDE NFLEHHSNIL AEIDDENEYN EYQLHTERWD KPVIATTMVQ
FLNSLFSGKS SSVRRMHRLC NSVIIIDEVQ SVPIKCVYLF TLAVNFLTKA CGCAVVLCSA
TQPTFEKNKH KLILDEKQDM IPDYQSDFDF FKRTELINAV IKHGFDNNEA AQFCYERFKE
NGDLLFVVNT KSEALRFYEL LKEKAENEAY IIHLSTNMCP AHRKKRIGTL RRFLKKHKPV
ICVTTQLIEA GVDISFRCVV RTLAGMDNAA QAAGRCNRNG EYERLCPVYL IDLKGENLGN
LSGIKNAQDI SRQILLYNSD DLLSAKVQSA YFEGLYKDSG EKMAYIPGKN KTSLLELLSL
NKEKYSACGK PEPDKYMAQS FKTAGSLFEV IDSRTRNVIV PYNKKAKDII AKLNSDISPE
DLKDLLRKAQ KYSVSIYSGM ERKLGDSGAV YTISSLNCEI TVLRDEFYSS EYGVTAEGSE
RELLVF
//
