ID A0A1K1RV28_9PSEU Unreviewed; 4774 AA.
AC A0A1K1RV28;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Acyl transferase domain-containing protein {ECO:0000313|EMBL:SFW75669.1};
GN ORFNames=SAMN04489730_3964 {ECO:0000313|EMBL:SFW75669.1};
OS Amycolatopsis australiensis.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=546364 {ECO:0000313|EMBL:SFW75669.1, ECO:0000313|Proteomes:UP000182740};
RN [1] {ECO:0000313|EMBL:SFW75669.1, ECO:0000313|Proteomes:UP000182740}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44671 {ECO:0000313|EMBL:SFW75669.1,
RC ECO:0000313|Proteomes:UP000182740};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FPJG01000006; SFW75669.1; -; Genomic_DNA.
DR RefSeq; WP_072477680.1; NZ_FPJG01000006.1.
DR STRING; 546364.SAMN04489730_3964; -.
DR OrthoDB; 9778690at2; -.
DR Proteomes; UP000182740; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 2.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR036299; Polyketide_synth_docking_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF18369; PKS_DE; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF101173; Docking domain B of the erythromycin polyketide synthase (DEBS); 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 6.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:SFW75669.1}.
FT DOMAIN 33..456
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1435..1510
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1526..1950
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2886..2961
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2978..3402
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4622..4697
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 4774 AA; 496704 MW; 944EB84FBB01E9BB CRC64;
MRNEDKLRDY LKRVTADLKL ANRRLHDVTE RRAEPIAIVA MSCRLPGGVS TPEQLSDLVA
EGRDAVSPWP ADRGWDPDLY DPDPERTGRS YVRDGCFLAD AAQFDPAFFG ISPREAVAMD
PQQRLLLETA WEAFERAKLD AAALRGSRTG VFVGASNQGY AGGLVTAPEG LEGLLLTGGS
AAVLSGRLAY TFGLEGPAVT VDTMCSSSLV ALHLAVSALR NDECAMALAC GATVMANPRN
FVEFSRQRGL SADGRCRAFS ADADGTGWGE GVGVLLLERL SDARRHGHPV LAVVRGTAIN
QDGASNGLTA PNGLAQQRVI EAALADARLA PEQVDAVEAH GTGTKLGDPI EAQALQATYG
RDRERPLLLG TVKSNIGHTQ AASGVAGVIK MVLALRAGLL PKTLFAGEPT PHVDWTAGRV
ALLTEPAEWP ETGAPRRAGV SSFGGSGTNA HVVLEGVAEE PPVAEESTAD TAVWVLSGRS
EAALKARAAD LAELPDANIA DVGWSLAHTR SAFEHRAAVV GRDLPALLAG VREIAGSGTA
AGVGGEATAV GGTVLVFPGQ GAQWAGMAVD LLGESPVFAE RMAECEAALA SFVDWKLTGV
LGDAAALERV DVVQPVSWAV MVSLAALWAA HGVTPDAVVG HSQGEIAAAV VAGALSLSDG
AKVVALRSKL IAGRLAGLGA MASVAAPEGR VAALLADGVS IAAVNGPASV VISGEVAAVE
AALARAEAEG LRVRRIPVDY ASHSAVVEVL ETELLEALAG LEPRPARITF VSSVTGEIAE
PLDAGYWYRN LRQRVRFQEA TETLVAAGHR FFVESSPHPV LGGGIQDTLD DTLEPGSAAV
VGTLRRGEGG LTRFLTSLAQ LWTRGRDVDW TAVHPGARPV ALPTYPFQRQ RYWLDTPAGT
AAADDGFWTA VEDGALTEQL GVTAETPWQD VLPALADWRR RRTELSTVDQ WRYRITWHPV
AEPTPAPAGT WLVVNAPDVC LDALEKAGAR VRTLRVGETD DRASLAERLR GETELAGVLS
LLALDERPHA THPVLTTGLA ANLLLSQALG DAGIAAPLWL ATQGAAVVVP GDELPHPGQA
TTWGLGRVLA LEHPDRWGGL VDLPETLDER AASRLCAVLT GIDAEDQLAV RESGIHLRRL
RRAPLTGTAP EWRTSGTALI TGGTGGLGAH VARWLAGRGA EHLVLTSRRG PDAPGAAELR
EELEALGVRV TIAACDVADR DALASLVSGL TEIRAVVHTA GVGLLAPLAA TTLPEFEEGA
RAKLAGARHL DALFGEAGLD AFVLYSSVAG VWGSGDHGAY AAANAYVDAL AESRRARGLA
GTSVAWGIWA GDGMVSDVVA DQLKWRGIPF MAPEPALLGL GQVLDHDEPF LAVADIDWAR
FVPVFTAARP RPLLSEVPEV AAVLAAEPEV IEDQTGAAGA LRAKLSGLSE PDQDRELLGL
VRKHAAAVLG YAGPDDVDAG RAFRELGFDS LTAIDLRNRL TAATGLKLSA TLVFDYPNAT
ALARHVRTEL LGVAVPAVAA AGTATDEPLA IVGMSCRFAG GVGSPQQLWE LMLAEADVIG
EFPGDRGWHP GTLYDPDPDR AGHSYTRAGG FLAGAAEFDP AFFGISPREA LAMDPQQRVV
MEASWEAFED AGLGQAAVRG SRTGVFVGAS GHDYGPRLRE VPEGVEGHLV TGGTGSVTSG
RVAYTFGLEG PAVTIDTGCS SSLVALHLAG QALRSGECDL ALAGGVAVMS TPAPFVAFSR
QRALAADGRS KPFSAEADGM GLAEGVGMLL VERLSDARRH GHRVLAVVRG TATNQDGASN
GLTAPNGPSQ QRVIRSALAA AGLEPSDVDA VEAHGTGTTL GDPIEAQALM ATYGRDRAEP
LWLGSVKSNI GHSQAAAGAA GVIKMVLAMR HGVLPRSLHS EHPSPHVDWA GGPLKLLTEA
TPWPDKPGPR RAGVSSFGVG GTNAHVVLEH APEPEPGVVR EPVAGPTPWL VSARAAAGVP
AQAEKLAAFA GDTDIADIGW SLATTRTAFE HRAVVLGDDR AELLAGLRDL ATGMPGASVV
TGVAAPAGRT VFVFPGQGAQ WAGMAVELLD SAPVFAERFA ECAAALESFV DWSPAAVLRG
AESLDRVDVV QPLSWAVMVS LAALWQSHGV TPDAVAGHSQ GEIAAAVVAG GLSLADGARV
AALRSKAIAA GLAGRGGMAS VALGAERVER LLAEWDGRIT IAAFNSPSST VVCGDPAALD
ELLAQCEADG VRARLIPVDY ASHSAHVAAI EAELLAALAP IEPRTGEIPF YSTVSAEPVD
TAALDAAYWY RNLRQPVLFG QTVEVLAARE HSVFVEVSPH PVVSAAIAET VPGAVVAGTL
RRGEGGPARF RRSLAELHVR GVEVDWAPVF PGAATVPLPT YAFQHQRYWL DAPARGAGAL
DDEFWAAVER ADLGALAAWR AERLLESTVD GWRYRVDWTP LTVASGTVVA NWLVVAPAGY
DARELADGLG ARLLVLPSDV DRARCAELLS EAGPCEGVLS LLALDGLEPN LTLLHALGDA
GIDAPLWWAT SGAVSTGAAD PIRAAAQALT WGFGRIAAME YPQRFGGLVD LPAELDARVP
ARLAAVLGTP SDEDQVAVRA SGVFGRRFVR AAPARATTTP WRPSGTVLLT GGTGGIGAHV
ARWLARAGAE RLVLTSRRGH APAGLAAELA DLGTELTVAA CDVADRGRLA ALLDGLPGLT
AVVHAAAVLD DGVIDGLTPE RIAAVLRPKA EAARHLHELT ADRDLSAFVL FSSLAGTLGG
PGQASYAAAN AYLDALARER RAAGLPATSI AWGAWGGGGA GEGLAGQGLP AMAPEPALLA
LQQALDEDET VLAVADIRWD RYRKTLALGR PGAALRGIPE AVASADGGGT GWAQRLAGLE
AAEQHRLVLE LIRAEAAVAL GYPGPASIEP DRAFRDLGFD SLTAVDLRNR LGEVTGLRLP
VTLVFDHPTA TVLAEHVRRE LAGDTAPDTA APVTAVTEDP IAIVAMSCRY PGGVADPEQL
WRLVAEGVDA VGEFPADRGW DLDRHYDADP DKAGTFYTRG GGFLHDAAEF DPAFFGIAPR
EAPAIDPQQR LLLETSWEAL ERAGLDPKLL RGSRTGVFVG SNYHDYGTRL RHAPGEFEGH
LATGSAGSVA SGRIAYTFGL EGPAVTVDTA CSSSLVALHW AVRALRSGEC DLALAGGVTV
ISSVDTFVEF SRQRALSPDG RCRAFSAGAD GAGWAEGAGM VLVERLSDAR RLGHPVLAVV
RGSAVNSDGA SNGLTAPSGP AQQRVIRAAL ADAGLPPSEV DAVEAHGTGT PLGDPIEAQA
LIATYGQDRE RPLWLGSVKS NIGHTQAASG IAGVIKMVQA LRHGVLPRTL HAETPSPHVD
WAAGHVRLLS EPVEWAANGH PRRAGVSSFG VSGTNVHAIL EEAPEVPGPP EAATAQRLTA
WPVSARSEAA LRAQARRLLA FTEPGTRSEV DAVDVARSLG ARTRFDHRAV VVGEDLAELR
AGLAALAADR PAAGLRRHTA RAGRTAFLFT GQGAQRPGMG RQLYERFPVF ADAFDAVCAH
LDPALRDVVF GDDAAPLDRT AVTQPALFAL EVALFRLLES WDVHADFLAG HSVGELAAAH
VAGVLSLADA AKLVSARGRL MEALPAGGAM VAIAAPEAEV VPLLGERVSL AAVNGPEAVV
VSGETEAVLA LKDHFAALGR KTRRLDVSHA FHSPLMEPML AEFAEVAAGL TYHRPRIPVV
AAGDVATPEH WVRHVREAVR FADAVATMTA EGVNRFVELG PDAVLAALVP DCAGPAELSV
GALLRKGHDE ERSLLTALAG LHVSGADVGW AAVRGGGRHV ELPTYPFQRE RYWLDAPAVT
GGLTAAGLGE TTHPLLGASV PLAGSDGALF TGLLSVASHP LLAQHVVLGT VLLPGTAFLE
LAVHAAEQVG CERVEELTLA APLVLPETGG VRLQVAVGGP DEYGNRPIGI HSRAGDALED
EPWTTHATGT LAVTPVPAPE PAGTWPPSGA EPIDLDGLYD RVAENGFGYG PAFRGLRRAW
RAGDEIFAEV ALPEAERAEA GRFGLHPALL DAALHTVALR DGSRAVLPFA WSGVTLHAGG
AAALRVRVSG RDQDEVTLAV AGEDGLPVAT VGSLTLRPVS AEQLDVARSA YHESLFRVGW
VLAPISPVSH PATERWAVVG VDVGVRVTKA LGTAGVPVES HPDFDALGAA VDAGAPVPGV
IVVTCGSSCG DDVPLAVRTA THGALEQVQE WLASERFAGS RLVFVTQRAI ATRVGEGITD
LATAAVVGLV RSAQSENPGR FVLVDLDDRD VSYAALPSAV ACGEPHVAVR VGAIQVPRLA
RVPATTAAPA AAWDPDGTVL ITGATGTLGT LVARHLVTAR GMRRLVLTSR RGPAAEGAKE
LVEELTAWGA DVTIAACDVA DRDSLAALLA AVPAEHPLTA VVHTAGVLDD GVVSALTTRQ
LDTALRPKVD AAWHLHELTA GMDLAGFVVF SSLAGTFGGT GQANYAAANA FLDALAHVRR
AAGLPALSLA WGLWAERSGM TGKLGEADLS RLARGGVEPM PSDEGLDLFD IACTLDEAVL
LPVRLVVEAM RDRVGEDAIP VILRSLIRTP VRRAVSAGVA PADPADSLTH RLAAMSEAER
EGALLDLVRT QAAAVLGYAG PDAIEPDRGF LELGFDSLSA VELRNLLGAA TGLRLPATLL
FDYPSTTVLA EYLRAELVPA VPAAILPALA ELDRVEIALP DLLADTQARE RLGTRLQELL
ARVTGAGAAT AVEERLESAT DDEMFALIDE LDLD
//
