ID A0A1K2IEA7_9FLAO Unreviewed; 948 AA.
AC A0A1K2IEA7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=SAMN05428642_1011095 {ECO:0000313|EMBL:SFZ90711.1};
OS Flaviramulus basaltis.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flaviramulus.
OX NCBI_TaxID=369401 {ECO:0000313|EMBL:SFZ90711.1, ECO:0000313|Proteomes:UP000182544};
RN [1] {ECO:0000313|EMBL:SFZ90711.1, ECO:0000313|Proteomes:UP000182544}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18180 {ECO:0000313|EMBL:SFZ90711.1,
RC ECO:0000313|Proteomes:UP000182544};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; FPKV01000001; SFZ90711.1; -; Genomic_DNA.
DR RefSeq; WP_072400707.1; NZ_FPKV01000001.1.
DR AlphaFoldDB; A0A1K2IEA7; -.
DR STRING; 369401.SAMN05428642_1011095; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000182544; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000182544};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 5..265
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 355..536
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 705..912
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT REGION 299..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..321
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 948 AA; 106592 MW; 43390276A207A9C9 CRC64;
MSDQKRLFLV DAYALIFRGY YAFIKNPRIN SKGVDTSAIM GFMNSLLDVI KRERPDHLAV
CFDKGGSADR VEMFEAYKAN RDATPEAIKI AVPYIQDILK AMHIPIMVKE GFEADDVIGT
LSKQAEKEGY KTFMVTPDKD FAQLVSENIF MYRPVFGGGY ETWGIPEVQK KFEVTDPLQV
IDFLGMMGDS SDNIPGLPGV GEKTAKKFLA QYGSMENLLA NTHELKGKMK EKIEAAKELG
MLSKKLATIM LDVPVEFHAE NFEMSHPDIP KVTEIFQELE FRRLIDNFTK AFSVEATAET
ATQETSSEAK ATPQETKSAG AGQFSLFGAD PSSSKEAEPV SEFTRKTTKT SSHFYQSVAS
GMATKLFVKN LMNQTSVCFD TETTSLNPLV AELVGIAFSW EVGKGFYLPF PENREEAQEL
IEQLRPFFES ENIEKIGQNL KYDIKVLAKY NIDVKGKFFD TMLAHYLINP DMRHSMDVLA
ETYLNYTPIP IEALIGKKGK NQGNMRDVPL EKQTEYAVED ADITLQLKEH FEKELGEANT
QKLFDDIEIP LLRVLAAMEL EGINLDKDFL NSLSEQLTID IKALETIIYT EAGEEFNIAS
PKQLGDILFD KMKLVDKPKK TKTGQYATSE DILSYLAKDH EIIQHILDFR GLSKLKSTYV
DALPNQVEPS TGRVHTDYMQ TVAATGRLSS NNPNLQNIPI RTERGRQVRK AFIPRNEDYT
LLAADYSQIE LRIIAALSKE ETMIEAFKNG EDIHASTASK VFNVPLEAVT REQRGNAKTV
NFGIIYGVSA FGLSNQTDLS RGEAKELIDT YYETYPKLRA YMSELVDFAR DNGYVQTVLG
RRRYLKDINS RNAVVRGAAE RNAVNAPIQG SAADIIKIAM INIYNKLEAG NYKTKMLLQV
HDELVFDVYK PELKDMKTLI KTEMENAFTL DVPLDVEVGV GADWLEAH
//
