ID A0A1L3KKE6_9VIRU Unreviewed; 2419 AA.
AC A0A1L3KKE6;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=Replicase {ECO:0000313|EMBL:APG77823.1};
OS Wenling hepe-like virus 1.
OC Viruses; Riboviria.
OX NCBI_TaxID=1923493 {ECO:0000313|EMBL:APG77823.1};
RN [1] {ECO:0000313|EMBL:APG77823.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WLJQ103981 {ECO:0000313|EMBL:APG77823.1};
RX PubMed=27880757; DOI=.1038/nature20167;
RA Shi M., Lin X.D., Tian J.H., Chen L.J., Chen X., Li C.X., Qin X.C., Li J.,
RA Cao J.P., Eden J.S., Buchmann J., Wang W., Xu J., Holmes E.C., Zhang Y.Z.;
RT "Redefining the invertebrate RNA virosphere.";
RL Nature 540:539-543(2016).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}.
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DR EMBL; KX883830; APG77823.1; -; Genomic_RNA.
DR RefSeq; YP_009337320.1; NC_033115.1.
DR GeneID; 30854855; -.
DR KEGG; vg:30854855; -.
DR Proteomes; UP000201097; Genome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd15457; NADAR; 1.
DR Gene3D; 3.40.220.10; Leucine Aminopeptidase, subunit E, domain 1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 1.10.357.40; YbiA-like; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002589; Macro_dom.
DR InterPro; IPR043472; Macro_dom-like.
DR InterPro; IPR012816; NADAR.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR037238; YbiA-like_sf.
DR PANTHER; PTHR11106; GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED; 1.
DR Pfam; PF01661; Macro; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR SMART; SM00506; A1pp; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52949; Macro domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF143990; YbiA-like; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51154; MACRO; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022807};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Protease {ECO:0000256|ARBA:ARBA00022807};
KW Reference proteome {ECO:0000313|Proteomes:UP000201097};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 82..268
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 988..1144
FT /note="Macro"
FT /evidence="ECO:0000259|PROSITE:PS51154"
FT REGION 892..941
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 964..997
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 981..997
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2419 AA; 274268 MW; 1C7E01EF739BAA7B CRC64;
MQGGPFEEAA GLRPVDGKVT SVTSAINAKG ADSRQLLNEY HSSELDKRLV SAVELPFIVS
PPTLDTMNEI FSPIVLRPSK SAVTNNVTNA HPFAAATTHL MQLELDRYNG PEFGGKISCR
MDHGMLENDD QDKKHVTCLK SDAKDDQRVI KALTLSERGS RLPQFLTQSG CVNGAENCKQ
PAKFLKCNNV LFWLTIKELF QLFKSRGTMR LLAGMLFPKG IETGETTTDY ENRIHLSIDK
VKNTAVMSFL DDNSLPYCHS LDTWQNYSRS GIIDGRKYDC NFNLLIERTK SFGIGSIVDI
TRVQRGLDIT VGVKTTTTTV KLRNFVTMLP QLAKYGDEID TCVGEISANF LELRRITSFF
EKMPIICVPE ALLTKLEQFL FNRKDADIDR GHVGAYLSAV LHEVTISHYQ IQKGNLLDLT
TFTDLCIVIL IRSFIARSKA SKSISFAALN ITKKSNSSFK SFCMSVESVF NDLKAFHAYR
SQGKEEKKKR PKRPDPYNQR RDLKTFAKKV MEHNLKAELV RKMIIEHEKT AYDVFKSVAK
THNLLHDFPT SRVLTFDSPT TGLCYQKVVT KLGILSQLKP FPTRGEIDAE LAEYEDEFDI
DFTDGHATLK VQSHDLCEHL VSMKNYNILP EPSDKHIDMQ HYYNKKLYSS VTDNTNYEKT
ALALTLLQPT IFEKLHNLAA SPMNDAGLWA NYHGKVVHWF NTKLNDALLP VPPIKQTIKF
NKNVLCADCL KEVDGPIYLD LGAEISHKYL PFMQLTVLQQ LIATKQRFVM KWQDFVGTME
NSRTYSKFGN TTIYDTLKKH YVYYSSCLSP GEVYVTNFDV TKLYTKNASV LASQTPLGRV
LESWNINLRS KHPVLNTKYS LSQKYIDDFE IVQFSDFNFI YKLEKNETPR FKDASTQTTK
PEYTITPTIT TELQSTTDSD FSDATSYTTT ESSGSSDWTT SSLESFDVDD WEVEEEKEFD
FPVTFAEEQQ PPPPPSIEPP SDTETNTTED SETSTSMNYS VEMVFGNIVD FEADYWVNSA
KETLEGGGGI DGAIHRAAGT TALKLANKGK YIVPGQVTVT SGFKSKAKQI IHTVAPRVND
EELYRKSVES ALNQVAQGKM VYLPYLGSGI FGLNKEMCKN LTTQIATEKA KERKIHIRVI
DLPYVKKDAL EIPKQKAKLE RKGALSDEIY DFLCGEMRGD IPRKCDRDNK FDFPTFLNGH
IPITHETLRF GGVIKSNKIF SNYAKTIVYN GDTSYPHNEG FYQHVLKSSY AKGITLKAAT
NPNPQVVKKA AEGNPMCCHE DAIIVMAKAV ESKFSRERLW KQLEQTNPKM LVEVSAKDRF
WASSYTGMGC NVLGKLLTLY RENTMFEEEE EVEEKKEEVK LEPPQQEALQ TIQEEEIDPQ
EEEAKQRAFV AKKQECTIET LSIRYYDATC ARPENDLTYK DCYPEPNTKY VLSCDAHAFD
KNEFMEQGKV LVFADSEHGK NLNMPFDGVS RAIVNGKLCG VVYEQTTFEF TPAVITFPHK
PEAIKRWERE LRTERQAEFK QVHKACAEFI KETTFKYTGN VHVMEGTYGS GKTFQLMKLI
REKQQNKNYM VVTPTSALAQ EYKHKKFNAC SWSTGLRNLY DMDELPKGFT IYIDEAYLID
PKVIHVLFSH AYKVYMIGDS RQLQPGDGKK ILDYPLLEEM LNVKPIKIMI AQKTPLDVVS
TLNAYEPQKT VTRSKIINSI NIVRVDNFSQ VPKICKPKCE LAHECCRGAV FDSYHAKCNG
IQTVAQIQGC RYNNFNLLLT SNSGQLINRA HGQKLVSISR HVEKLTLYES RDAQLHNKFG
FIKPMLDKTI IAGRNDVPTI FGAYDLNDVK YREPSGVEAN KVKISKQTTP TDFASAKGVT
RLAMQRDIKE KVGREVTLTI PTVTQVQAAV HDFPHFPVST ITDNSEEIRV EMDFEQNMLE
KGTFTKILGM HPDAPAIDHL IKKVSPTNAP LASGMRDINI ATMREIDESK MLKFKNHTAL
FQEKQGTTIA GLASMFGHVQ KNITPDHSIN TAIERAASKP KKVDGPEVRS RAKALINGVS
KFLDLDKLKP PTLEEFALCR AQAEMRVAAK RTQPEPSMYG DLHSTTAAIK CFNKQQMKSK
VGENSWLTTK EDDLGLYIKG GQMVSAQSKN VNQITAGWVN WAERIVFSSL SPYVVPGYGR
SPRTFRRKVL NIIKQHREEF ECKSIDLSEQ DTIKDGATNL LMREIYARCG VPSHIMDILE
SPNHNWRMKT LFSETSLKYH FQSGRMDTLF SNTIHVLAEV GKSYDIYDPI LVLAQGDDVY
IRASSIRKTL DFTPQIKEDN NQIGDFIGFL IGKDEVFPDL PRIAQKHVSK SHDTKERREE
LRVAIKDLMS CFSTNVCYYQ NMLYSVAYKY NLTTADVAIF YDYLEHQAVD DSPFKEKQNL
DRLILTAIPV YFETQRAQL
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