ID A0A1L3KLT4_9VIRU Unreviewed; 2672 AA.
AC A0A1L3KLT4;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Genome polyprotein {ECO:0008006|Google:ProtNLM};
OS Hubei picorna-like virus 74.
OC Viruses; Riboviria.
OX NCBI_TaxID=1923158 {ECO:0000313|EMBL:APG78371.1};
RN [1] {ECO:0000313|EMBL:APG78371.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WGML145648 {ECO:0000313|EMBL:APG78371.1};
RX PubMed=27880757; DOI=.1038/nature20167;
RA Shi M., Lin X.D., Tian J.H., Chen L.J., Chen X., Li C.X., Qin X.C., Li J.,
RA Cao J.P., Eden J.S., Buchmann J., Wang W., Xu J., Holmes E.C., Zhang Y.Z.;
RT "Redefining the invertebrate RNA virosphere.";
RL Nature 540:539-543(2016).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}.
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DR EMBL; KX884252; APG78371.1; -; Genomic_RNA.
DR RefSeq; YP_009337019.1; NC_032986.1.
DR GeneID; 30854454; -.
DR KEGG; vg:30854454; -.
DR Proteomes; UP000201714; Genome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23169; ps-ssRNAv-Picornavirales; 1.
DR Gene3D; 1.20.960.20; -; 1.
DR Gene3D; 2.60.120.20; -; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.510.10; Positive stranded ssRNA viruses; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 1.
DR InterPro; IPR004005; Calicivirus_coat.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR004004; Helic/Pol/Pept_Calicivir-typ.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF00915; Calici_coat; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR PRINTS; PR00918; CALICVIRUSNS.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF88633; Positive stranded ssRNA viruses; 1.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022806};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000201714};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 365..526
FT /note="SF3 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51218"
FT DOMAIN 1290..1492
FT /note="Peptidase C3"
FT /evidence="ECO:0000259|PROSITE:PS51874"
FT DOMAIN 1790..1926
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT REGION 1149..1171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2672 AA; 302796 MW; B99A687070FAAD7A CRC64;
MSCSKRALID CDVVKDCIVP NVESRETDLS SFYQLCFIEP NKQSTDVEDN SNLEEDLKSV
SQCYNYSDSE EGDVLSSVLS LNSAVFSVLS RAKLLNLIDQ LSNVFVADSF VSALAPLARI
CELYGYLWSD VNGQMGVLEI LWNVLVWMKD LIVPLFKGSK LVPDDVKSRL PDTEPVKQAF
VKLSEDRKGK IKSFFENIGC HELAGRVPYL VPVLATVVTG ALAFVAFRFG PAMLQQKEMN
FVKSVTAMGM LARSSHFLRA EFASIWKVAT DCFAEIFGLA WVEDADIEVK KYISDLHSLY
KEFRQYQNRI LSDPYVVFGD QEFLPRLELQ LKRLDDFYLR LASAKKNMVN AKSALDSLRE
MAKESRLLVD NIKRTCTEKI EPVVIWLSGA AGIGKSRAVS KIVEELGKLH GEVLHTYVRG
TADQYWSGYS GQPVVIYDDF GNSKEKTEFT ELVSIKTCQA FLLNCAAVEE KGKLFTSKYV
IICANMLDVV NTAQIPNVEV LNRRRDFLFV VENPELTNYV QVNGREPTAD CDIWKDDFSH
LTIRRRAALP RDRDAVRDNS EWDVFRIAQE SYVRFRTAEQ KFYHYLTDLQ TRRQRLQDAV
EDKDGLPSAP VCAVRGGGES SVSDSEVLER FGFLFDTDEP ERQFFHQFER QIHMPILFVG
EAGTGKTFTM HAIRRRFEQE KLKVRMFSGT QFQTFFDGGG KVTKYDVILI EDFTVTAEAM
NAFRTFVNEA EDGFFGKKIV VATGNLSLVH DHAAGRGVDW LAAFERRVRI YQFSFKRRWL
FKYTPADVVA NPENYDVMVR ISASTLTTQE RSDLFQRDLI DFVVQATPRV ALYQTFESLP
KIGEVEATFE VVLKVTPSDF LKWMKSKKGV YTPAGVAAAI AEVHSMISVP FSKMVFFGRF
LHSMAAKGIG LSLMSGWRTE RNMLTLRDIL VNVANLNIEF PTSEVVRFRY ADEAYLLCAS
DEGRLCILEE CPDGFVRQTG SFVGGVDYTK DEKEFMQRVS HVIQRQRVAM KEKQDKCGKA
FERMIVSGGL KTLPAWARAL FEAVNVLLLY GTAVVTLQDS YVRYRQLQDL KEKKNLLDEF
CTPELEGQKP SKVEITETFV LKDPKVKGQP EKVSTVVHVN GKSYREAGMA SWGSDSSLSS
NVSLPPMMRA PKPTARRSAG GGGGTSVTLQ DTPTYATKSY SYMRNLEVAK PGLRLRENSV
APVTTVASSA QPIMAARYAD VVQPSQYDLD RIANMQRVYE KNGLQRTVLM PSPTLQKKLE
TIVESEEISN EVLDQLNCEE FEVQRESQSD PQAHDLAELC IVNSVSIVYA DSKEHCVYGL
MIAEDVGVTV LHCASACLNV EILSTPGVYY DMVLLSKDRT RDLMIFRVQS CPAKFRNITN
HLRSELYTTE IDGHDAMLVT PSSRNRVGWK RWAVVRACVS RKYAGSDVVE HERTYVGMLA
GYKIVTGVGT ASGDCGSSLI LNSARLPFKL IGIHKAATDK VGYAAEIFRE DFQEFVPIKE
PNRQRALKRD EIELFQEPMP VGRTTQIVGR AVFRNHQSVK TQFYRCPLAC PFLDDEFEPV
ILSHQDFRCR DTDFYPEDAF LKWDGPTGFE IDCAPFSADV RAQIFEMVSD QICDKWIFEC
ERLHRPLFKL TKTEAINRPS QCIGLSNSLN RASSPGYPFR HFGARRGKAD FLEFDEGQQI
WMLAKNEHGR LLNHSIDLFV DEARRGNVPP IVYVANLKDE TRKKDRIYEQ PKTRGFAAAP
VHYVIAGRMY NYAAMALCAE ARAQLGHVLG MDPNSLEWHS MTLRLVSKSN CIINGDYKDF
DSGHFREALA LPTRIRCELY RRLDPGWRPE DTIIRENIDR ASQYGLLLRG HLLFQHSKGI
FSGQFDTTFN NCVLNQLYIV YAWFVTMFEK KRFDLMSLGG FDKMCHLEVC GDDFNLSISD
LDLFGLQDIV RVLSQHFGIV VTSASAKDST QSVLPYEDIY EVDFLKRQYV CIGGRWCGRV
KMSVFDKMLN WCVGPKRCLT PENLYDEFFD MTNMCALMDV ALIEAVFWGR ELYEKLVAHF
RSFLCQPGID IQKDLPSYDM MCILFELVPS DMRVFGNVCR NQIAVTDKQM ATVKNAKPSA
AGVEPQNINV PDVPVSPPDQ MSPYAPTATN ATQQASQGIG CVPLSVPHIV GKYVWNETIS
ITTNDEPGKV LWWRALHPST NPYADWLTKP FNAWAGGMLF RATICANFLC AGKVVLVHWM
PNQNPTLERT LEEVTCQPHM MCDLKNMETT ELLVEDENPT KWHYYQPMPQ MNTDPDGNIT
PSEWFSSLGV GGYIAMYVYG RFNMGEGSLN SLDIIVESAV APSFTVNYLK SVRSSDTPKP
GVAQEAWQTL KDASIPFCPT SPHPNKIIVT PQPVFGFDTV AQMTCGGTFW DTNWWKGDFE
LAADRATNVG QVNLIPTGTT QSGPTFTATY DTFVNEFVPN KISRVLYCIR GGKFYDWTVG
SYFNWNIETR KYEGAKMTYT MEDAGLPASD NRPHLIGTYS MDDEFEYPAY PEDTKKFVPG
VPESLIMFGR YDQNYPFPSA SFQTQEMVKA FYSKTSKIAS QITPTTAGLF MIYDTKNRLP
VRQCKLYYEG FLTVMASDKY EIMDSDYELQ FMSIVDRNYV IKDKQNLIEN SEFAKNQDRI
RKATAFLERT DLTKLERLAG IVTQHWGSLT RR
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