ID A0A1L3KMB3_9VIRU Unreviewed; 2079 AA.
AC A0A1L3KMB3;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=RNA-directed RNA polymerase {ECO:0008006|Google:ProtNLM};
OS Wenzhou picorna-like virus 35.
OC Viruses; Riboviria.
OX NCBI_TaxID=1923621 {ECO:0000313|EMBL:APG78528.1};
RN [1] {ECO:0000313|EMBL:APG78528.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WZRBX39547 {ECO:0000313|EMBL:APG78528.1};
RX PubMed=27880757; DOI=.1038/nature20167;
RA Shi M., Lin X.D., Tian J.H., Chen L.J., Chen X., Li C.X., Qin X.C., Li J.,
RA Cao J.P., Eden J.S., Buchmann J., Wang W., Xu J., Holmes E.C., Zhang Y.Z.;
RT "Redefining the invertebrate RNA virosphere.";
RL Nature 540:539-543(2016).
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DR EMBL; KX884344; APG78528.1; -; Genomic_RNA.
DR RefSeq; YP_009336976.1; NC_032969.1.
DR GeneID; 30854395; -.
DR KEGG; vg:30854395; -.
DR Proteomes; UP000202909; Genome.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23194; Dicistroviridae_RdRp; 1.
DR Gene3D; 1.20.960.20; -; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR000199; Peptidase_C3A/C3B_picornavir.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00548; Peptidase_C3; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Covalent protein-RNA linkage {ECO:0000256|ARBA:ARBA00022520};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000202909};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT DOMAIN 640..817
FT /note="SF3 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51218"
FT DOMAIN 1264..1484
FT /note="Peptidase C3"
FT /evidence="ECO:0000259|PROSITE:PS51874"
FT DOMAIN 1780..1924
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT REGION 900..958
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2060..2079
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 80..145
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 901..940
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 941..958
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2079 AA; 232699 MW; 85F62F5437316C15 CRC64;
MCNLVSVFCQ KSKLSGVASF NGQHLIWCLI TMASTTHSKD CVDSIPSPAR KVKGRSPVEE
LATAFNSKLR LVNSGNSKQI ALLTRKEKKL TARLEFLRER HKHNKEEISR LIKQIAFIRQ
EIDYLKRWQN RIQSDRKAFR QLRRDAKAMF YEDEAEAQGW SSAATDKTDF VFRPMRLTPF
TQECETAVSC PYRIVPPDAS WERRTEPRDR NFACVALRAD KEYMAFRQKC NSREIQITND
VPFFVKTGEY SVSTQTNVDE NLSMAEWTSD KQAPSYGFSS IKLRTNFETR HLHIDGVVEK
YSSVAREFST TEREAGYFGF MLPVENVAHM VHRSQLTGSE LDLADEIIEE IVAEAQAGET
QGGIVSKARQ EVKQLSDTVK DTGVAARSVT QTSDCIRNFI DMMTEQGSMI FTNLAKITIM
DINMVFIGFA QLLLAAYNGS FAQCFLAVSQ IAMSLGVAGR ITAIWEEFSN YMRGTEEEER
TDVAEAQSYE KVVGQASSTL SSFTLLGGSL MALVVAGACK TKQVNEFMQS MRLFGQAKHG
FNCAKDCFMW LYEWARDAFC RWQFGVSSET IDLQNIEPHI EEIITNAQLI RKWDIGLYKL
SKDLCAATIA TDDSLRQLET KLTLANMPLP ALTVKTLQND LRGHTLAALT SPSRSDLNRC
RPFSLWIYGA SGVGKSVLVK WFQQKYAAKY LETDSSAISD YVFARKSANE YWDGYKNQPI
VYYDDALQTK DSTTMPNPEI MELIYAINDA PYHLHMASLS QKTSSYFDSQ FVLCTSNTRC
PSPVSIKDRK AFTRRFDLAI EITVKPKFAT KGALDSAKVS KYMVEHARDE SEFCEAVYEI
RTYDMHTGNY DTTYNLSTFW HAFCDAKEKC DKHRIALKNS MGIKKEDSLM DKAAQLAKSL
KPVAETQSKE TKITTSPAST SSDSSSASTT PIPPLSDSSS ESEGSEEEDS ESEEEEEYVD
IDADVVCGCF FNKCYEKQIL EDPGINDCNC VYKDWFCNKV TYGSKIIKEK ISISDASTKS
PEDVRQTLDS LSSCVIEKTK TAMSAGPLCE QLKQEVKAGS ISTFEDMMAH LKTDKEVSIK
FPTFAKVTEE AKRLKEYISS QLARLHKAGT KIYEIFQAIF NSKTARWLGV VAAGLAGIGY
FNVTRCKVLA ARTLDGLKNA TLCRSSCDAC ATFNPAKAEA GNKKGPLCYL RGWCKKLFST
TKDPKFAEMA FVAESQSREI LTKAAARKTT AIVQADEIPV SDSANFLAKT GLEPKVEAKA
QSGDVVLVEQ WEKIVKHNLV VLRSTIITSE GETLIRTINA LYISGRTLMT PAHWFKTQCG
PTFELLNYGS ITGPEIRVRD IAITYVKDLR GREQDMCMFT APPNIRCGAK ITNFFAPAAD
IHKIKRAKML LSGYKHLGEH KVLFTSTVTK QQILDYEVGI TTRSGPNTTC KAHDVIFYDV
TTEQGMCGSL LFAACKGIPG KIIGMHYAGL KGDGISIVLS RELIQNAITK HTDDYEIDTY
GFVDVKVPSY VDTGTQPAVQ AIAQGDCHYV GKAKPVPVSG KTQISPSILA GNLGECITKP
AHLRPFQLSD GTTIDPMTKG IAKMLGKVPT INVSILKEAL SDTYITFFAS LPEDSKGIGV
LTYEEAIDGV IRNEELREKA TYVKPLNRSS SAGYPWVLQN KGQGKHHWFG YDDKYLYSDE
VRETVMRMIN SASQGCCEVA VYCATLKDER RPIEKVNAGK TRVFCAAPMH FVIACRMYFL
GFANEIMSRH KISECAVGID PYSLSWDFMY HKLCSLGNGR NIIAGDFSNF DGSLRGDILA
KVCDLICYWY TTRTDMAGGF DNCDNLIRHV LFENIQSHHV LVGSDVYQMT HSQPSGNPLT
VIINSIYNMI VCRYAYYYCL KEFLLTPEGG NTDDALCAFH DNVVLVAYGD DNLLSVSERA
KCFFTPQNMA RALLTIGHIY TSSDKTSELH FQTVEEVSFL KRKFKYHNEL QRFVAALDTD
VIAEMCRWVR GKEKEEATKQ NVHDAAREAV LHGLHFYESF RTWISSACRA NEFFVDLLPY
DLALAHFKGD MAGEECDSKN SLGSLESRQD GLNDSGQYI
//
