ID A0A1L3KMB6_9VIRU Unreviewed; 2456 AA.
AC A0A1L3KMB6;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=Genome polyprotein {ECO:0000256|ARBA:ARBA00020107};
OS Wenzhou picorna-like virus 24.
OC Viruses; Riboviria.
OX NCBI_TaxID=1923609 {ECO:0000313|EMBL:APG78560.1};
RN [1] {ECO:0000313|EMBL:APG78560.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WZSBei69493 {ECO:0000313|EMBL:APG78560.1};
RX PubMed=27880757; DOI=.1038/nature20167;
RA Shi M., Lin X.D., Tian J.H., Chen L.J., Chen X., Li C.X., Qin X.C., Li J.,
RA Cao J.P., Eden J.S., Buchmann J., Wang W., Xu J., Holmes E.C., Zhang Y.Z.;
RT "Redefining the invertebrate RNA virosphere.";
RL Nature 540:539-543(2016).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}.
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DR EMBL; KX884362; APG78560.1; -; Genomic_RNA.
DR RefSeq; YP_009337258.1; NC_033088.1.
DR GeneID; 30854777; -.
DR KEGG; vg:30854777; -.
DR Proteomes; UP000202952; Genome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:InterPro.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd00205; rhv_like; 2.
DR Gene3D; 2.60.120.20; -; 3.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 1.
DR InterPro; IPR014872; Dicistrovirus_capsid-polyPr_C.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR004004; Helic/Pol/Pept_Calicivir-typ.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001676; Picornavirus_capsid.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR033703; Rhv-like.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF08762; CRPV_capsid; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00073; Rhv; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR PRINTS; PR00918; CALICVIRUSNS.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF88633; Positive stranded ssRNA viruses; 3.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 4: Predicted;
KW Capsid protein {ECO:0000256|ARBA:ARBA00022561};
KW Covalent protein-RNA linkage {ECO:0000256|ARBA:ARBA00022520};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000202952};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Virion {ECO:0000256|ARBA:ARBA00022561}.
FT TRANSMEM 636..659
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 709..731
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 256..433
FT /note="SF3 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51218"
FT DOMAIN 1287..1414
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 2456 AA; 276728 MW; 8E67FC0645FE6342 CRC64;
MSVDDITKLF SSVIDLFKDI PSQVRDFDGL DIPSLLTSII SYGVQLYTGS SLAGYVASTV
SFIMSICPKV SHALNLAKEF VVKNFQYLMA WIKGVTLQTE SGTTIVQSVL SGLLSNPFCV
SFRDMILSLV SARIFSKDVS AKISKVFGKP KSMSLLEVCS VVVDGISNMV KFASMLLEGH
SFSEIWNASD PLSTFIQESE DLLRFKDHLN RGFRVEGYML YSDFRLKAQE LIDVSPTIIS
SKSPYDSKIK IAKSLTQDLI AATIEVDRTV NSQKRLPPYA FILCGPPGIG KSHLLSLIYE
IYGEAVGKPF EDSQVYARNK NTTYWEGYIP FAQHIIYYTE LGNESKSYVK TRGDPLLNEI
QSLVDSNPYP VDMAFDNKGK FFAVPEVIAI DTNNRELHVG ELMYSKSAIK RRFLYIDVSV
LKEFRCSKGT GIDTQKSIDA GGNYLNRYSF RCTRYAPNGN DSVIEKVLFK GEDVVEFTRF
LMGDIRTYVS KQKEMISKDL TNFVKEIGRN EKDQIKELSE SDCCSDFNGQ EFHDSIDLKQ
QEFGHENLEN YEVEVFAESA ILEEENQPHD ENLPQPNSNL LSKSKKDILR SAIKQGWDDF
KTYSKLLFSS IWLYFLCCCA RGYRRDDPLL DRFIGSHIWI IVPFFVTYFV WNTIFKIIFT
TVSMFGYFSI RHFGLKQLGH YNVIGVKLVR SQAKAHVSHL LFGTEYNPFI IPSWATIGFV
FTIAVVVFKS YSLYSSYNKK QESESSQFFS DDAISSILQE KEDELGCGRS KARVRVNGQN
ATWNLADISY SKPVHNGSPL ELYNSVSRNL RFVVVSSSKA QRPTHIFGVC QHFAIINKHA
TCGDKEFKIF HFPKGKHDKG AGFEEYIITK DDIVPFRDDL LLIRVVGMRF RNFLPHFLKS
PHPQRFGGAV GSVESSMLYH KGTINVLDNF SEVTINGYIS YEREYVDGMC GVPLVGAVTR
SGCSIVGIHC AGNKECKTRI GVAIMQSDVE KAIAKLKNKK DMFEVLSASV LKSESLCLPG
LKSPFKFEHL PHINYLGSTG KPVFMNNKSN IVKSGLYDSL DAIFDTIGFE PDEHFVVPTL
KPRMVGEEYI SPWNLSLKKI NQTSPPLDMR IIDRIVKEYS ERIISMIKAK HGNDYSLNPL
DIQTAVNGHE RDAYLRRINP STSGGYGYGA KDPHIPIVHE EDSIREATPE LKTRINEILE
TYENGDSANF IYGTSLKDEP RDAKKNAIGK IRVFYMTSLD NLIVSRMFLA PFYTMMVEDG
EIFRTSVGTN MHSDADAIYE QMRAFSNDII EGDYSNFDVT IPFGIGHAAA SVVLRVLKAF
GYNTMALNMT EGILSDALFS LVHMNGDIFF KPGLQPSGKY GTAEDNSLRG VLMQMYIYYT
IPALWKTSFF RETMLFTYGD DYLNGLSKKA AEHLNNFVYR DKCLEHFNMK VTPASKAGVM
TEFVTPDDMS FLRRTWVWDP VHKVHNAKLN MNSIYKSLQW TAPSQIESVA DQELSTIRSA
LWELFFHLDR DTHHKFRNLL VEAYCLAHKV PINGVLQDLP TWTNIESKLF PEFQSESGEV
RAKATDMLNA LELEEVKEQY TAEELHLYKS LWSDPKVDVR AMKRNVSAQA DAVATKVFLD
RFLKKKSMPD FYTESGELSS GLVSDSKRIL TQNFEDMVGE GEKTVEMSSR PLHNGQYTLL
QANDFLSRPV ELANFSIPLS SDLGVAFPIW DLFTLEEAVR AKLRNYAYLR GDLCVRITVS
GSPFHSGRLL VSYQPCANRN ANLTALNASP NYRTMLLNYL SQAPESGIID VKANKPLMLR
CPYISTKPAF RLWNESSSVI AATTSYADIE EAGRLYLYTM NQVKAVSDSP STPYVQIVAW
MENVQLGCPT ATQIEISTES GNMEDDEFEA GPVEKFSSAA VQVSDALKVV PYIAPFATAS
SFIFNGMKTF SSIFGWSKPP IVGDPIFVKN RPFANGCQTI GASTVKRIGL DPKQEVSIDP
SPCGVDQDDM IIANIAARES YFTTFTWAPT DSILSNSIFK CRVHPQLDTV YRGFGLKDYY
QPTALSFAST PFTFWRGDLV FRFDFVCSAY HRGKLVVYYE PNISQHALMD LDIDLNKQWI
QVIDIQETQS VEFCVKWAAS RPWMRLMNAP KSILNYQSFT NSSAYQEVNG YIGVAVLTEI
QSPDDSSIEV NVFVRGENMR FNALTEANMP SERMVLDPFD MGLMPPELFN VTTESGMVKD
NSDQPVSCIS LNDSTATDDW ISKMYFGEEP VSFRSLLKRY VETRHASVDV GTSSDRCVIA
TSNIMPNIDP PYGSAISGEK TLLSYLRYAY LGTKGGMRKR VRLLGNSSFD SPYLRTSISL
LEFGGSYLEN FEWTSSHAQV KQMGTVSFVP HTNGGLEAEI PFYSPNLFSY SFADNLIGDN
PTGDMSVSWY KRYICQFESM DGSVGGAEFI EETATAEDFM FMRFMGAPCF SNDKIL
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