ID A0A1L3MWT6_9BACI Unreviewed; 136 AA.
AC A0A1L3MWT6;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Probable nitronate monooxygenase {ECO:0000256|ARBA:ARBA00013457};
DE AltName: Full=Propionate 3-nitronate monooxygenase {ECO:0000256|ARBA:ARBA00031155};
GN ORFNames=A9C19_20210 {ECO:0000313|EMBL:APH06809.1};
OS Bacillus weihaiensis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=1547283 {ECO:0000313|EMBL:APH06809.1, ECO:0000313|Proteomes:UP000181936};
RN [1] {ECO:0000313|EMBL:APH06809.1, ECO:0000313|Proteomes:UP000181936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Alg07 {ECO:0000313|EMBL:APH06809.1,
RC ECO:0000313|Proteomes:UP000181936};
RX PubMed=27901120; DOI=10.1038/srep38248;
RA Zhu Y., Chen P., Bao Y., Men Y., Zeng Y., Yang J., Sun J., Sun Y.;
RT "Complete genome sequence and transcriptomic analysis of a novel marine
RT strain Bacillus weihaiensis reveals the mechanism of brown algae
RT degradation.";
RL Sci. Rep. 6:38248-38248(2016).
CC -!- FUNCTION: Nitronate monooxygenase that uses molecular oxygen to
CC catalyze the oxidative denitrification of alkyl nitronates. Acts on
CC propionate 3-nitronate (P3N), the presumed physiological substrate.
CC Probably functions in the detoxification of P3N, a metabolic poison
CC produced by plants and fungi as a defense mechanism.
CC {ECO:0000256|ARBA:ARBA00003535}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 3 O2 + 3 propionate 3-nitronate = 3 3-oxopropanoate + 3
CC H(+) + H2O2 + 2 nitrate + nitrite; Xref=Rhea:RHEA:57332,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:136067;
CC Evidence={ECO:0000256|ARBA:ARBA00000219};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the nitronate monooxygenase family. NMO class I
CC subfamily. {ECO:0000256|ARBA:ARBA00009881}.
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DR EMBL; CP016020; APH06809.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1L3MWT6; -.
DR STRING; 1547283.A9C19_20210; -.
DR KEGG; bwh:A9C19_20210; -.
DR OrthoDB; 9778912at2; -.
DR Proteomes; UP000181936; Chromosome.
DR GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR004136; NMO.
DR PANTHER; PTHR42747; NITRONATE MONOOXYGENASE-RELATED; 1.
DR PANTHER; PTHR42747:SF3; NITRONATE MONOOXYGENASE-RELATED; 1.
DR Pfam; PF03060; NMO; 1.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
PE 3: Inferred from homology;
KW Detoxification {ECO:0000256|ARBA:ARBA00022575};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000181936}.
FT DOMAIN 1..128
FT /note="Nitronate monooxygenase"
FT /evidence="ECO:0000259|Pfam:PF03060"
SQ SEQUENCE 136 AA; 15146 MW; 679634E4DD2E511B CRC64;
MDGRGLMASI CLGAEGVQMG TAFLTCIESG ASEIHKEEVL HTPENQTIMT RSFSGKWARG
IKNEFIFEMQ KSEELLPNFP VQNTLTKGIR KAATLQKNRE FMSLWSGQSP RLAKKQTVKS
LIESIVEEAT LILSNK
//
