ID A0A1L3ZWA5_9SPHN Unreviewed; 334 AA.
AC A0A1L3ZWA5;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=cholesterol 7-desaturase {ECO:0000256|ARBA:ARBA00026095};
DE EC=1.14.19.21 {ECO:0000256|ARBA:ARBA00026095};
GN ORFNames=BSL82_11680 {ECO:0000313|EMBL:API59890.1}, BSL82_11870
GN {ECO:0000313|EMBL:API59921.1};
OS Tardibacter chloracetimidivorans.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Tardibacter.
OX NCBI_TaxID=1921510 {ECO:0000313|EMBL:API59890.1, ECO:0000313|Proteomes:UP000182063};
RN [1] {ECO:0000313|Proteomes:UP000182063}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JJ-A5 {ECO:0000313|Proteomes:UP000182063};
RA Lee H., Ka J.-O.;
RT "Complete Genome Sequence of alachlor-degrading Sphingomonas sp. strain JJ-
RT A5.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:API59890.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JJ-A5 {ECO:0000313|EMBL:API59890.1};
RX PubMed=29721829;
RA Lee H., Kim D.U., Park S., Yoon J.H., Ahn J.H., Ka J.O.;
RT "Tardibacter chloracetimidivorans gen. nov., sp. nov., a novel member of
RT the family Sphingomonadaceae isolated from an agricultural soil from Jeju
RT Island in Republic of Korea.";
RL J. Microbiol. 56:324-330(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + H(+) + NADH + O2 = 7-dehydrocholesterol + 2 H2O
CC + NAD(+); Xref=Rhea:RHEA:51644, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16113, ChEBI:CHEBI:17759,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.19.21;
CC Evidence={ECO:0000256|ARBA:ARBA00029355};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51645;
CC Evidence={ECO:0000256|ARBA:ARBA00029355};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + H(+) + NADPH + O2 = 7-dehydrocholesterol + 2 H2O
CC + NADP(+); Xref=Rhea:RHEA:45024, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:17759, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.14.19.21; Evidence={ECO:0000256|ARBA:ARBA00029369};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45025;
CC Evidence={ECO:0000256|ARBA:ARBA00029369};
CC -!- PATHWAY: Hormone biosynthesis. {ECO:0000256|ARBA:ARBA00004972}.
CC -!- PATHWAY: Steroid hormone biosynthesis; dafachronic acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00025712}.
CC -!- SIMILARITY: Belongs to the cholesterol 7-desaturase family.
CC {ECO:0000256|ARBA:ARBA00025729}.
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DR EMBL; CP018221; API59890.1; -; Genomic_DNA.
DR EMBL; CP018221; API59921.1; -; Genomic_DNA.
DR RefSeq; WP_072597680.1; NZ_CP018221.1.
DR AlphaFoldDB; A0A1L3ZWA5; -.
DR STRING; 1921510.BSL82_11680; -.
DR KEGG; sphj:BSL82_11680; -.
DR KEGG; sphj:BSL82_11870; -.
DR OrthoDB; 9800776at2; -.
DR Proteomes; UP000182063; Chromosome.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0044270; P:cellular nitrogen compound catabolic process; IEA:UniProt.
DR GO; GO:0008203; P:cholesterol metabolic process; IEA:InterPro.
DR GO; GO:1901565; P:organonitrogen compound catabolic process; IEA:UniProt.
DR Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR InterPro; IPR045605; KshA-like_C.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR PANTHER; PTHR21266:SF60; CHOLESTEROL 7-DESATURASE; 1.
DR PANTHER; PTHR21266; IRON-SULFUR DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF19298; KshA_C; 1.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF55961; Bet v1-like; 1.
DR SUPFAM; SSF50022; ISP domain; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000182063}.
FT DOMAIN 16..117
FT /note="Rieske"
FT /evidence="ECO:0000259|PROSITE:PS51296"
SQ SEQUENCE 334 AA; 38093 MW; 0933FCAD13E0734E CRC64;
MDLSGLPFSN RPVGWFQIGW SGEFAPGTAH PLRYFGHDLV AFRTLVGELS VLDGHCPHLG
AHLGYGGKVK GDCVACPYHG WEWDTEGRNA RIPYQDSPMK MSLRKWPVRE QHGCVFMWHD
PTGAPPRWAL PHVFESYPAL PGREEDYYPP YGNSTARFDR ESVHTQVTME NGVDSVHFRH
THNAPVDPVL EDWVIRDAEL HTRVGFLSPA TGNVALTLHN ITAGVGLSFS AFEGSYHYRL
VFAGTPVDDD TTDYHYTIWY PREPGDTGSI MPPDLRQRIK SQFLYTLEED MLIWRTQAYV
PRPTYAKQDI KPYTAMRKWQ KNFYNLPPLN QNSA
//
