ID A0A1L6JD15_9SPHN Unreviewed; 335 AA.
AC A0A1L6JD15;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Biotin synthase {ECO:0000256|ARBA:ARBA00012236, ECO:0000256|HAMAP-Rule:MF_01694};
DE EC=2.8.1.6 {ECO:0000256|ARBA:ARBA00012236, ECO:0000256|HAMAP-Rule:MF_01694};
GN Name=bioB {ECO:0000256|HAMAP-Rule:MF_01694,
GN ECO:0000313|EMBL:RSU17870.1};
GN ORFNames=BRX38_15300 {ECO:0000313|EMBL:RSU38834.1}, BRX39_18975
GN {ECO:0000313|EMBL:RSU30460.1}, BRX40_16465
GN {ECO:0000313|EMBL:APR53798.1}, CA221_13065
GN {ECO:0000313|EMBL:RSU49443.1}, CA222_16950
GN {ECO:0000313|EMBL:RSU23230.1}, CA223_11340
GN {ECO:0000313|EMBL:RSU39595.1}, CA224_18740
GN {ECO:0000313|EMBL:RSU17870.1}, CA225_18800
GN {ECO:0000313|EMBL:RSU23323.1}, CA226_21420
GN {ECO:0000313|EMBL:RSV57765.1}, CA227_19780
GN {ECO:0000313|EMBL:RSX07514.1}, CA228_14790
GN {ECO:0000313|EMBL:RSV55476.1}, CA229_15450
GN {ECO:0000313|EMBL:RSV53879.1}, CA230_18900
GN {ECO:0000313|EMBL:RSW91491.1}, CA231_17115
GN {ECO:0000313|EMBL:RSV44256.1}, CA232_18510
GN {ECO:0000313|EMBL:RSV34161.1}, CA238_16895
GN {ECO:0000313|EMBL:RSV21838.1}, CA239_18975
GN {ECO:0000313|EMBL:RSV24406.1}, CA240_13560
GN {ECO:0000313|EMBL:RSV05294.1}, CA242_18145
GN {ECO:0000313|EMBL:RSV04595.1}, CA253_19655
GN {ECO:0000313|EMBL:RSU84809.1}, CA256_02345
GN {ECO:0000313|EMBL:RSU98807.1}, CA257_04545
GN {ECO:0000313|EMBL:RSV06190.1}, DAH52_18930
GN {ECO:0000313|EMBL:RSU91409.1}, DAH53_15900
GN {ECO:0000313|EMBL:RSU78130.1}, DAH54_14545
GN {ECO:0000313|EMBL:RSU78722.1}, DAH57_17140
GN {ECO:0000313|EMBL:RSZ19097.1}, DAH58_18540
GN {ECO:0000313|EMBL:RSZ09520.1}, DAH59_13405
GN {ECO:0000313|EMBL:RSY96671.1}, DAH60_16635
GN {ECO:0000313|EMBL:RSZ07809.1}, DAH61_16745
GN {ECO:0000313|EMBL:RSY95901.1}, DAH62_19015
GN {ECO:0000313|EMBL:RSY91580.1}, DAH63_18880
GN {ECO:0000313|EMBL:RSZ05475.1}, DAH64_17960
GN {ECO:0000313|EMBL:RSY79255.1}, DAH65_19955
GN {ECO:0000313|EMBL:RSY80392.1}, DAH67_18730
GN {ECO:0000313|EMBL:RSY68290.1}, DAH68_16445
GN {ECO:0000313|EMBL:RSY67779.1}, DAH69_16655
GN {ECO:0000313|EMBL:RSY59549.1}, DAH70_18220
GN {ECO:0000313|EMBL:RSY50158.1}, DAH71_18035
GN {ECO:0000313|EMBL:RSY51012.1}, DAH72_17225
GN {ECO:0000313|EMBL:RSY59942.1}, DAH73_18210
GN {ECO:0000313|EMBL:RSY31367.1}, DAH74_15715
GN {ECO:0000313|EMBL:RSY43977.1}, DAH75_18275
GN {ECO:0000313|EMBL:RSY34729.1}, DAH76_17295
GN {ECO:0000313|EMBL:RSY38745.1}, DAH77_16015
GN {ECO:0000313|EMBL:RSY21674.1}, DAH78_18535
GN {ECO:0000313|EMBL:RSY08473.1}, DAH79_16685
GN {ECO:0000313|EMBL:RSY24819.1}, DAH80_17975
GN {ECO:0000313|EMBL:RSY17299.1}, DAH81_18420
GN {ECO:0000313|EMBL:RSY08567.1}, DAH82_16385
GN {ECO:0000313|EMBL:RSX99055.1}, DAH83_14090
GN {ECO:0000313|EMBL:RSY04864.1}, DAH84_04935
GN {ECO:0000313|EMBL:RSX84895.1}, DAH85_19175
GN {ECO:0000313|EMBL:RSX87459.1}, DAH86_11785
GN {ECO:0000313|EMBL:RSX87193.1}, DAH87_18505
GN {ECO:0000313|EMBL:RSX92871.1}, DAH88_16820
GN {ECO:0000313|EMBL:RSX60782.1}, DAH89_17550
GN {ECO:0000313|EMBL:RSX70846.1}, DAH90_14230
GN {ECO:0000313|EMBL:RSX76801.1}, DAH91_14740
GN {ECO:0000313|EMBL:RSX60121.1}, DAH92_18685
GN {ECO:0000313|EMBL:RSX63444.1}, DAH93_16260
GN {ECO:0000313|EMBL:RSX42815.1}, DAH94_15365
GN {ECO:0000313|EMBL:RSX41340.1}, DAH95_18775
GN {ECO:0000313|EMBL:RSX36018.1}, DAH96_18415
GN {ECO:0000313|EMBL:RSX17214.1}, DAH97_19005
GN {ECO:0000313|EMBL:RSX26556.1}, DAH98_07405
GN {ECO:0000313|EMBL:RSX28120.1}, DAH99_16655
GN {ECO:0000313|EMBL:RSX20200.1}, DAI00_20355
GN {ECO:0000313|EMBL:RSX03522.1}, DAI01_15135
GN {ECO:0000313|EMBL:RSX04867.1};
OS Sphingomonas koreensis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=93064 {ECO:0000313|EMBL:APR53798.1, ECO:0000313|Proteomes:UP000185161};
RN [1] {ECO:0000313|EMBL:APR53798.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ABOJV {ECO:0000313|EMBL:APR53798.1};
RA Conlan S., Thomas P.J., Mullikin J., Palmore T.N., Frank K.M., Segre J.A.;
RT "Whole genome sequencing of Sphingomonas koreensis.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000185161}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ABOJV {ECO:0000313|Proteomes:UP000185161};
RA Conlan S., Thomas P.J., Mullikin J., Palmore T.N., Frank K.M., Segre J.A.;
RT "Whole genome sequencing of Sphingomonas sp. ABOJV.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000286970, ECO:0000313|Proteomes:UP000287271}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK-NIH.Env2_1116 {ECO:0000313|EMBL:RSU23230.1,
RC ECO:0000313|Proteomes:UP000286970}, SK-NIH.Env3_1116
RC {ECO:0000313|EMBL:RSU39595.1, ECO:0000313|Proteomes:UP000287271},
RC SK-NIH.Env4_1116 {ECO:0000313|EMBL:RSU17870.1,
RC ECO:0000313|Proteomes:UP000287347}, SK-NIH.Env8_1116
RC {ECO:0000313|EMBL:RSU23323.1, ECO:0000313|Proteomes:UP000287976},
RC SK-NIH.Pt2_0716 {ECO:0000313|EMBL:RSU38834.1,
RC ECO:0000313|Proteomes:UP000287307}, SK-NIH.Pt4_0816
RC {ECO:0000313|EMBL:RSU30460.1, ECO:0000313|Proteomes:UP000287414}, and
RC SK-NIH.Pt6_1016 {ECO:0000313|EMBL:RSU49443.1,
RC ECO:0000313|Proteomes:UP000287315};
RA Johnson R.C., Deming C., Conlan S., Zellmer C., Michelin A., Lee-Lin S.-Q.,
RA Thomas P.J., Park M., Weingarten R.A., Less J., Dekker J.P., Frank K.M.,
RA Musser K.A., Mcquiston J.R., Henderson D.K., Lau A.F., Palmore T.N.,
RA Segre J.A.;
RT "Genomic and Epidemiologic Investigation to Identify Sphingomonas koreensis
RT Point Sources in an Indolent Hospital Outbreak.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|Proteomes:UP000286681, ECO:0000313|Proteomes:UP000286696}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK-NIH.Env10_0317 {ECO:0000313|EMBL:RSV06190.1,
RC ECO:0000313|Proteomes:UP000286681}, SK-NIH.Env11_0417
RC {ECO:0000313|EMBL:RSU78722.1, ECO:0000313|Proteomes:UP000287165},
RC SK-NIH.Env12_0417 {ECO:0000313|EMBL:RSU91409.1,
RC ECO:0000313|Proteomes:UP000288342}, SK-NIH.Env15_0417
RC {ECO:0000313|EMBL:RSU78130.1, ECO:0000313|Proteomes:UP000288648},
RC SK-NIH.Env1_1016 {ECO:0000313|EMBL:RSV57765.1,
RC ECO:0000313|Proteomes:UP000288283}, SK-NIH.Env5_1116
RC {ECO:0000313|EMBL:RSV55476.1, ECO:0000313|Proteomes:UP000287812},
RC SK-NIH.Env7_1116 {ECO:0000313|EMBL:RSX07514.1,
RC ECO:0000313|Proteomes:UP000288130}, SK-NIH.Env9_0217
RC {ECO:0000313|EMBL:RSU84809.1, ECO:0000313|Proteomes:UP000287377},
RC SK-NIH.Pt10_0210 {ECO:0000313|EMBL:RSV04595.1,
RC ECO:0000313|Proteomes:UP000288286}, SK-NIH.Pt11_0411
RC {ECO:0000313|EMBL:RSW91491.1, ECO:0000313|Proteomes:UP000288535},
RC SK-NIH.Pt12_0112 {ECO:0000313|EMBL:RSV53879.1,
RC ECO:0000313|Proteomes:UP000286696}, SK-NIH.Pt14_0612
RC {ECO:0000313|EMBL:RSV34161.1, ECO:0000313|Proteomes:UP000287457},
RC SK-NIH.Pt16_1112 {ECO:0000313|EMBL:RSV44256.1,
RC ECO:0000313|Proteomes:UP000287806}, SK-NIH.Pt7_0906
RC {ECO:0000313|EMBL:RSV21838.1, ECO:0000313|Proteomes:UP000288528},
RC SK-NIH.Pt8_0208 {ECO:0000313|EMBL:RSV24406.1,
RC ECO:0000313|Proteomes:UP000287886}, SK-NIH.Pt9_0208
RC {ECO:0000313|EMBL:RSV05294.1, ECO:0000313|Proteomes:UP000287793}, and
RC SK-NY1_0417 {ECO:0000313|EMBL:RSU98807.1,
RC ECO:0000313|Proteomes:UP000288253};
RA Johnson R.C., Deming C., Conlan S., Zellmer C.J., Michelin A.V.,
RA Lee-Lin S., Thomas P.J., Park M., Weingarten R.A., Less J., Dekker J.P.,
RA Frank K.M., Musser K.A., Mcquiston J.R., Henderson D.K., Lau A.F.,
RA Palmore T.N., Segre J.A.;
RT "Genomic and Epidemiologic Investigation of an Indolent Hospital
RT Outbreak.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|Proteomes:UP000286706, ECO:0000313|Proteomes:UP000286739}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK-NIH.Env13_0417 {ECO:0000313|EMBL:RSZ09520.1,
RC ECO:0000313|Proteomes:UP000287513}, SK-NIH.Env14_0417
RC {ECO:0000313|EMBL:RSY96671.1, ECO:0000313|Proteomes:UP000288569},
RC SK-NIH.Env16_0517 {ECO:0000313|EMBL:RSZ07809.1,
RC ECO:0000313|Proteomes:UP000287015}, SK-NIH.Env17_0517
RC {ECO:0000313|EMBL:RSZ19097.1, ECO:0000313|Proteomes:UP000287799},
RC SK-NIH.Env18_0517 {ECO:0000313|EMBL:RSY95901.1,
RC ECO:0000313|Proteomes:UP000288230}, SK-NIH.Env19_0617
RC {ECO:0000313|EMBL:RSY79255.1, ECO:0000313|Proteomes:UP000288258},
RC SK-NIH.Env20_0617 {ECO:0000313|EMBL:RSY80392.1,
RC ECO:0000313|Proteomes:UP000287482}, SK-NIH.Env21_0617
RC {ECO:0000313|EMBL:RSY91580.1, ECO:0000313|Proteomes:UP000287363},
RC SK-NIH.Env22_0617 {ECO:0000313|EMBL:RSZ05475.1,
RC ECO:0000313|Proteomes:UP000287001}, SK-NIH.Env23_0717
RC {ECO:0000313|EMBL:RSY68290.1, ECO:0000313|Proteomes:UP000287360},
RC SK-NIH.Env24_0717 {ECO:0000313|EMBL:RSY67779.1,
RC ECO:0000313|Proteomes:UP000287229}, SK-NIH.Env25_0717
RC {ECO:0000313|EMBL:RSY59549.1, ECO:0000313|Proteomes:UP000288269},
RC SK-NIH.Env26_0717 {ECO:0000313|EMBL:RSY50158.1,
RC ECO:0000313|Proteomes:UP000287417}, SK-NIH.Env27_0717
RC {ECO:0000313|EMBL:RSY51012.1, ECO:0000313|Proteomes:UP000286788},
RC SK-NIH.Env28_0717 {ECO:0000313|EMBL:RSY59942.1,
RC ECO:0000313|Proteomes:UP000288630}, SK-NIH.Env29_0717
RC {ECO:0000313|EMBL:RSY31367.1, ECO:0000313|Proteomes:UP000287038},
RC SK-NIH.Env30_0717 {ECO:0000313|EMBL:RSY43977.1,
RC ECO:0000313|Proteomes:UP000288207}, SK-NIH.Env31_0717
RC {ECO:0000313|EMBL:RSY34729.1, ECO:0000313|Proteomes:UP000287340},
RC SK-NIH.Env32_0717 {ECO:0000313|EMBL:RSY38745.1,
RC ECO:0000313|Proteomes:UP000288007}, SK-NIH.Env33_0717
RC {ECO:0000313|EMBL:RSY21674.1, ECO:0000313|Proteomes:UP000287541},
RC SK-NIH.Env34_0717 {ECO:0000313|EMBL:RSY08473.1,
RC ECO:0000313|Proteomes:UP000288649}, SK-NIH.Env35_0717
RC {ECO:0000313|EMBL:RSY24819.1, ECO:0000313|Proteomes:UP000287968},
RC SK-NIH.Env36_0717 {ECO:0000313|EMBL:RSX99055.1,
RC ECO:0000313|Proteomes:UP000288516}, SK-NIH.Env37_0717
RC {ECO:0000313|EMBL:RSY17299.1, ECO:0000313|Proteomes:UP000287028},
RC SK-NIH.Env38_0817 {ECO:0000313|EMBL:RSY08567.1,
RC ECO:0000313|Proteomes:UP000287257}, SK-NIH.Env39_0817
RC {ECO:0000313|EMBL:RSY04864.1, ECO:0000313|Proteomes:UP000287073},
RC SK-NIH.Env40_0817 {ECO:0000313|EMBL:RSX84895.1,
RC ECO:0000313|Proteomes:UP000288050}, SK-NIH.Env41_0817
RC {ECO:0000313|EMBL:RSX87459.1, ECO:0000313|Proteomes:UP000286739},
RC SK-NIH.Env42_0817 {ECO:0000313|EMBL:RSX87193.1,
RC ECO:0000313|Proteomes:UP000288181}, SK-NIH.Env43_0817
RC {ECO:0000313|EMBL:RSX92871.1, ECO:0000313|Proteomes:UP000286795},
RC SK-NIH.Env44_0817 {ECO:0000313|EMBL:RSX60782.1,
RC ECO:0000313|Proteomes:UP000286792}, SK-NIH.Env45_1017
RC {ECO:0000313|EMBL:RSX70846.1, ECO:0000313|Proteomes:UP000286760},
RC SK-NIH.Env46_1017 {ECO:0000313|EMBL:RSX63444.1,
RC ECO:0000313|Proteomes:UP000287577}, SK-NIH.Env47_1017
RC {ECO:0000313|EMBL:RSX42815.1, ECO:0000313|Proteomes:UP000288581},
RC SK-NIH.Env48_1017 {ECO:0000313|EMBL:RSX76801.1,
RC ECO:0000313|Proteomes:UP000287090}, SK-NIH.Env49_1017
RC {ECO:0000313|EMBL:RSX60121.1, ECO:0000313|Proteomes:UP000286744},
RC SK-NIH.Env50_1017 {ECO:0000313|EMBL:RSX41340.1,
RC ECO:0000313|Proteomes:UP000288198}, SK-NIH.Env51_1017
RC {ECO:0000313|EMBL:RSX36018.1, ECO:0000313|Proteomes:UP000287844},
RC SK-NIH.Env52_1017 {ECO:0000313|EMBL:RSX17214.1,
RC ECO:0000313|Proteomes:UP000287083}, SK-NIH.Env53_1017
RC {ECO:0000313|EMBL:RSX26556.1, ECO:0000313|Proteomes:UP000288435},
RC SK-NIH.Env54_1217 {ECO:0000313|EMBL:RSX20200.1,
RC ECO:0000313|Proteomes:UP000288203}, SK-NIH.Env55_1217
RC {ECO:0000313|EMBL:RSX03522.1, ECO:0000313|Proteomes:UP000286706},
RC SK-NIH.Env56_1217 {ECO:0000313|EMBL:RSX04867.1,
RC ECO:0000313|Proteomes:UP000287020}, and SK-NIH.Env57_1217
RC {ECO:0000313|EMBL:RSX28120.1, ECO:0000313|Proteomes:UP000287236};
RA Johnson R.C., Deming C., Conlan S., Zellmer C.J., Michelin A.V.,
RA Lee-Lin S.-Q., Thomas P.J., Park M., Weingarten R.A., Less J., Dekker J.P.,
RA Frank K.M., Musser K.A., Mcquiston J.R., Henderson D.K., Lau A.F.,
RA Palmore T.N., Segre J.A.;
RT "Genomic and Epidemiologic Investigation of an Indolent Hospital
RT Outbreak.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the conversion of dethiobiotin (DTB) to biotin by
CC the insertion of a sulfur atom into dethiobiotin via a radical-based
CC mechanism. {ECO:0000256|HAMAP-Rule:MF_01694}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-
CC 2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine +
CC [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-
CC [ferredoxin]; Xref=Rhea:RHEA:22060, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739,
CC ChEBI:CHEBI:17319, ChEBI:CHEBI:29917, ChEBI:CHEBI:33737,
CC ChEBI:CHEBI:33738, ChEBI:CHEBI:57586, ChEBI:CHEBI:57844,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:149473; EC=2.8.1.6;
CC Evidence={ECO:0000256|ARBA:ARBA00023417, ECO:0000256|HAMAP-
CC Rule:MF_01694};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01694};
CC Note=Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3
CC cysteines and 1 arginine. {ECO:0000256|HAMAP-Rule:MF_01694};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR001619-1};
CC Note=Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3
CC cysteines and 1 arginine. {ECO:0000256|PIRSR:PIRSR001619-1};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01694,
CC ECO:0000256|PIRSR:PIRSR001619-1};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|HAMAP-Rule:MF_01694, ECO:0000256|PIRSR:PIRSR001619-1};
CC -!- PATHWAY: Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-
CC diaminononanoate: step 2/2. {ECO:0000256|ARBA:ARBA00004942,
CC ECO:0000256|HAMAP-Rule:MF_01694}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01694}.
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. Biotin synthase
CC family. {ECO:0000256|ARBA:ARBA00010765, ECO:0000256|HAMAP-
CC Rule:MF_01694}.
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DR EMBL; CP018820; APR53798.1; -; Genomic_DNA.
DR EMBL; QLJD01000013; RSU17870.1; -; Genomic_DNA.
DR EMBL; QLJF01000013; RSU23230.1; -; Genomic_DNA.
DR EMBL; QLJC01000017; RSU23323.1; -; Genomic_DNA.
DR EMBL; QLJI01000019; RSU30460.1; -; Genomic_DNA.
DR EMBL; QLJJ01000012; RSU38834.1; -; Genomic_DNA.
DR EMBL; QLJE01000005; RSU39595.1; -; Genomic_DNA.
DR EMBL; QLJG01000008; RSU49443.1; -; Genomic_DNA.
DR EMBL; QQWM01000010; RSU78130.1; -; Genomic_DNA.
DR EMBL; QQWL01000010; RSU78722.1; -; Genomic_DNA.
DR EMBL; QQWQ01000019; RSU84809.1; -; Genomic_DNA.
DR EMBL; QQWN01000022; RSU91409.1; -; Genomic_DNA.
DR EMBL; QQWP01000002; RSU98807.1; -; Genomic_DNA.
DR EMBL; QQWR01000016; RSV04595.1; -; Genomic_DNA.
DR EMBL; QQWS01000007; RSV05294.1; -; Genomic_DNA.
DR EMBL; QQWO01000003; RSV06190.1; -; Genomic_DNA.
DR EMBL; QQWU01000012; RSV21838.1; -; Genomic_DNA.
DR EMBL; QQWT01000016; RSV24406.1; -; Genomic_DNA.
DR EMBL; QQXA01000017; RSV34161.1; -; Genomic_DNA.
DR EMBL; QQXB01000011; RSV44256.1; -; Genomic_DNA.
DR EMBL; QQXD01000020; RSV53879.1; -; Genomic_DNA.
DR EMBL; QQXE01000011; RSV55476.1; -; Genomic_DNA.
DR EMBL; QQXG01000020; RSV57765.1; -; Genomic_DNA.
DR EMBL; QQXC01000020; RSW91491.1; -; Genomic_DNA.
DR EMBL; QQXR01000016; RSX03522.1; -; Genomic_DNA.
DR EMBL; QQXQ01000009; RSX04867.1; -; Genomic_DNA.
DR EMBL; QQXF01000030; RSX07514.1; -; Genomic_DNA.
DR EMBL; QQXV01000012; RSX17214.1; -; Genomic_DNA.
DR EMBL; QQXS01000012; RSX20200.1; -; Genomic_DNA.
DR EMBL; QQXU01000012; RSX26556.1; -; Genomic_DNA.
DR EMBL; QQXT01000004; RSX28120.1; -; Genomic_DNA.
DR EMBL; QQXW01000017; RSX36018.1; -; Genomic_DNA.
DR EMBL; QQXX01000011; RSX41340.1; -; Genomic_DNA.
DR EMBL; QQXY01000013; RSX42815.1; -; Genomic_DNA.
DR EMBL; QQYA01000013; RSX60121.1; -; Genomic_DNA.
DR EMBL; QQYD01000014; RSX60782.1; -; Genomic_DNA.
DR EMBL; QQXZ01000017; RSX63444.1; -; Genomic_DNA.
DR EMBL; QQYC01000013; RSX70846.1; -; Genomic_DNA.
DR EMBL; QQYB01000011; RSX76801.1; -; Genomic_DNA.
DR EMBL; QQYH01000003; RSX84895.1; -; Genomic_DNA.
DR EMBL; QQYF01000008; RSX87193.1; -; Genomic_DNA.
DR EMBL; QQYG01000017; RSX87459.1; -; Genomic_DNA.
DR EMBL; QQYE01000014; RSX92871.1; -; Genomic_DNA.
DR EMBL; QQYJ01000012; RSX99055.1; -; Genomic_DNA.
DR EMBL; QQYI01000012; RSY04864.1; -; Genomic_DNA.
DR EMBL; QQYN01000015; RSY08473.1; -; Genomic_DNA.
DR EMBL; QQYK01000014; RSY08567.1; -; Genomic_DNA.
DR EMBL; QQYL01000013; RSY17299.1; -; Genomic_DNA.
DR EMBL; QQYO01000010; RSY21674.1; -; Genomic_DNA.
DR EMBL; QQYM01000012; RSY24819.1; -; Genomic_DNA.
DR EMBL; QQYS01000010; RSY31367.1; -; Genomic_DNA.
DR EMBL; QQYQ01000013; RSY34729.1; -; Genomic_DNA.
DR EMBL; QQYP01000012; RSY38745.1; -; Genomic_DNA.
DR EMBL; QQYR01000008; RSY43977.1; -; Genomic_DNA.
DR EMBL; QQYV01000013; RSY50158.1; -; Genomic_DNA.
DR EMBL; QQYU01000013; RSY51012.1; -; Genomic_DNA.
DR EMBL; QQYW01000011; RSY59549.1; -; Genomic_DNA.
DR EMBL; QQYT01000010; RSY59942.1; -; Genomic_DNA.
DR EMBL; QQYX01000013; RSY67779.1; -; Genomic_DNA.
DR EMBL; QQYY01000012; RSY68290.1; -; Genomic_DNA.
DR EMBL; QQZB01000014; RSY79255.1; -; Genomic_DNA.
DR EMBL; QQZA01000016; RSY80392.1; -; Genomic_DNA.
DR EMBL; QQZD01000014; RSY91580.1; -; Genomic_DNA.
DR EMBL; QQZE01000014; RSY95901.1; -; Genomic_DNA.
DR EMBL; QQZG01000011; RSY96671.1; -; Genomic_DNA.
DR EMBL; QQZC01000016; RSZ05475.1; -; Genomic_DNA.
DR EMBL; QQZF01000011; RSZ07809.1; -; Genomic_DNA.
DR EMBL; QQZH01000014; RSZ09520.1; -; Genomic_DNA.
DR EMBL; QQZI01000013; RSZ19097.1; -; Genomic_DNA.
DR RefSeq; WP_075152337.1; NZ_QQZI01000013.1.
DR AlphaFoldDB; A0A1L6JD15; -.
DR STRING; 93064.BRX40_16465; -.
DR GeneID; 44134156; -.
DR KEGG; skr:BRX40_16465; -.
DR OrthoDB; 9786826at2; -.
DR UniPathway; UPA00078; UER00162.
DR Proteomes; UP000185161; Chromosome.
DR Proteomes; UP000286681; Unassembled WGS sequence.
DR Proteomes; UP000286696; Unassembled WGS sequence.
DR Proteomes; UP000286706; Unassembled WGS sequence.
DR Proteomes; UP000286739; Unassembled WGS sequence.
DR Proteomes; UP000286744; Unassembled WGS sequence.
DR Proteomes; UP000286760; Unassembled WGS sequence.
DR Proteomes; UP000286788; Unassembled WGS sequence.
DR Proteomes; UP000286792; Unassembled WGS sequence.
DR Proteomes; UP000286795; Unassembled WGS sequence.
DR Proteomes; UP000286970; Unassembled WGS sequence.
DR Proteomes; UP000287001; Unassembled WGS sequence.
DR Proteomes; UP000287015; Unassembled WGS sequence.
DR Proteomes; UP000287020; Unassembled WGS sequence.
DR Proteomes; UP000287028; Unassembled WGS sequence.
DR Proteomes; UP000287038; Unassembled WGS sequence.
DR Proteomes; UP000287073; Unassembled WGS sequence.
DR Proteomes; UP000287083; Unassembled WGS sequence.
DR Proteomes; UP000287090; Unassembled WGS sequence.
DR Proteomes; UP000287165; Unassembled WGS sequence.
DR Proteomes; UP000287229; Unassembled WGS sequence.
DR Proteomes; UP000287236; Unassembled WGS sequence.
DR Proteomes; UP000287257; Unassembled WGS sequence.
DR Proteomes; UP000287271; Unassembled WGS sequence.
DR Proteomes; UP000287307; Unassembled WGS sequence.
DR Proteomes; UP000287315; Unassembled WGS sequence.
DR Proteomes; UP000287340; Unassembled WGS sequence.
DR Proteomes; UP000287347; Unassembled WGS sequence.
DR Proteomes; UP000287360; Unassembled WGS sequence.
DR Proteomes; UP000287363; Unassembled WGS sequence.
DR Proteomes; UP000287377; Unassembled WGS sequence.
DR Proteomes; UP000287414; Unassembled WGS sequence.
DR Proteomes; UP000287417; Unassembled WGS sequence.
DR Proteomes; UP000287457; Unassembled WGS sequence.
DR Proteomes; UP000287482; Unassembled WGS sequence.
DR Proteomes; UP000287513; Unassembled WGS sequence.
DR Proteomes; UP000287541; Unassembled WGS sequence.
DR Proteomes; UP000287577; Unassembled WGS sequence.
DR Proteomes; UP000287793; Unassembled WGS sequence.
DR Proteomes; UP000287799; Unassembled WGS sequence.
DR Proteomes; UP000287806; Unassembled WGS sequence.
DR Proteomes; UP000287812; Unassembled WGS sequence.
DR Proteomes; UP000287844; Unassembled WGS sequence.
DR Proteomes; UP000287886; Unassembled WGS sequence.
DR Proteomes; UP000287968; Unassembled WGS sequence.
DR Proteomes; UP000287976; Unassembled WGS sequence.
DR Proteomes; UP000288007; Unassembled WGS sequence.
DR Proteomes; UP000288050; Unassembled WGS sequence.
DR Proteomes; UP000288130; Unassembled WGS sequence.
DR Proteomes; UP000288181; Unassembled WGS sequence.
DR Proteomes; UP000288198; Unassembled WGS sequence.
DR Proteomes; UP000288203; Unassembled WGS sequence.
DR Proteomes; UP000288207; Unassembled WGS sequence.
DR Proteomes; UP000288230; Unassembled WGS sequence.
DR Proteomes; UP000288253; Unassembled WGS sequence.
DR Proteomes; UP000288258; Unassembled WGS sequence.
DR Proteomes; UP000288269; Unassembled WGS sequence.
DR Proteomes; UP000288283; Unassembled WGS sequence.
DR Proteomes; UP000288286; Unassembled WGS sequence.
DR Proteomes; UP000288342; Unassembled WGS sequence.
DR Proteomes; UP000288435; Unassembled WGS sequence.
DR Proteomes; UP000288516; Unassembled WGS sequence.
DR Proteomes; UP000288528; Unassembled WGS sequence.
DR Proteomes; UP000288535; Unassembled WGS sequence.
DR Proteomes; UP000288569; Unassembled WGS sequence.
DR Proteomes; UP000288581; Unassembled WGS sequence.
DR Proteomes; UP000288630; Unassembled WGS sequence.
DR Proteomes; UP000288648; Unassembled WGS sequence.
DR Proteomes; UP000288649; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0004076; F:biotin synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009102; P:biotin biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR HAMAP; MF_01694; BioB; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR010722; BATS_dom.
DR InterPro; IPR002684; Biotin_synth/BioAB.
DR InterPro; IPR024177; Biotin_synthase.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR00433; bioB; 1.
DR PANTHER; PTHR22976; BIOTIN SYNTHASE; 1.
DR PANTHER; PTHR22976:SF2; BIOTIN SYNTHASE, MITOCHONDRIAL; 1.
DR Pfam; PF06968; BATS; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR PIRSF; PIRSF001619; Biotin_synth; 1.
DR SFLD; SFLDG01060; BATS_domain_containing; 1.
DR SFLD; SFLDF00272; biotin_synthase; 1.
DR SMART; SM00876; BATS; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|ARBA:ARBA00022714, ECO:0000256|HAMAP-Rule:MF_01694};
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_01694};
KW Biotin biosynthesis {ECO:0000256|ARBA:ARBA00022756, ECO:0000256|HAMAP-
KW Rule:MF_01694};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01694};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP-
KW Rule:MF_01694};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01694}; Reference proteome {ECO:0000313|Proteomes:UP000185161};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01694};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_01694, ECO:0000313|EMBL:RSU17870.1}.
FT DOMAIN 42..266
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT BINDING 57
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
FT BINDING 61
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
FT BINDING 64
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
FT BINDING 101
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
FT BINDING 132
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
FT BINDING 192
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
FT BINDING 270
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01694,
FT ECO:0000256|PIRSR:PIRSR001619-1"
SQ SEQUENCE 335 AA; 36371 MW; 9AB1E503A03DA249 CRC64;
MTADVMMRTD WTREEIAALF DLPFPELLFR AAEVHRAQFA ADEVQLSTLL SIKTGGCPED
CGYCNQSTHA ETGLKATKLM DVRAVLQAAA QAKDNGSSRF CMGAAWRNPK DRDMPAIVEM
VKGVRQMGME TCMTLGMLSE KQAAQLADAG LDYYNHNIDT SPERYAEVIT TRTFEDRLDT
LEHVREAGIN VCCGGIVGMG ETRGDRVGFI HALATLPKHP ESVPVNALVP VKGTVLGNML
ADTPLAKIDE IEFVRTIAVA RITMPESMVR LSAGRESMSD STQALCFMAG ANSIFTGDKL
LTTGNAGDDK DAALFARLGM RPMQGEVKVE LEAAE
//
