ID A0A1L7I273_9FLAO Unreviewed; 1507 AA.
AC A0A1L7I273;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=Glutamate synthase [NADPH] large chain {ECO:0000313|EMBL:APU67681.1};
DE EC=1.4.1.13 {ECO:0000313|EMBL:APU67681.1};
GN ORFNames=C723_3613 {ECO:0000313|EMBL:OSS37477.1}, GRFL_0957
GN {ECO:0000313|EMBL:APU67681.1};
OS Christiangramia flava JLT2011.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Christiangramia.
OX NCBI_TaxID=1229726 {ECO:0000313|EMBL:APU67681.1, ECO:0000313|Proteomes:UP000186230};
RN [1] {ECO:0000313|EMBL:OSS37477.1, ECO:0000313|Proteomes:UP000194120}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JLT2011 {ECO:0000313|EMBL:OSS37477.1,
RC ECO:0000313|Proteomes:UP000194120};
RX PubMed=24048873; DOI=10.1099/ijs.0.051987-0;
RA Liu K., Li S., Jiao N., Tang K.;
RT "Gramella flava sp. nov., a member of the family Flavobacteriaceae isolated
RT from seawater.";
RL Int. J. Syst. Evol. Microbiol. 64:165-168(2014).
RN [2] {ECO:0000313|EMBL:APU67681.1, ECO:0000313|Proteomes:UP000186230}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JLT2011 {ECO:0000313|EMBL:APU67681.1,
RC ECO:0000313|Proteomes:UP000186230};
RA Tang K.;
RT "Multi-omics approach to identify versatile polysaccharide utilization
RT systems of a marine flavobacterium Gramella flava.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Evidence={ECO:0000256|ARBA:ARBA00001927};
CC -!- PATHWAY: Amino-acid biosynthesis. {ECO:0000256|ARBA:ARBA00029440}.
CC -!- SIMILARITY: Belongs to the glutamate synthase family.
CC {ECO:0000256|ARBA:ARBA00009716}.
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DR EMBL; CP016359; APU67681.1; -; Genomic_DNA.
DR EMBL; AMRU01000026; OSS37477.1; -; Genomic_DNA.
DR STRING; 1229726.GRFL_0957; -.
DR KEGG; gfl:GRFL_0957; -.
DR OrthoDB; 9758182at2; -.
DR Proteomes; UP000186230; Chromosome.
DR Proteomes; UP000194120; Unassembled WGS sequence.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0004355; F:glutamate synthase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006537; P:glutamate biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd00982; gltB_C; 1.
DR CDD; cd00713; GltS; 1.
DR CDD; cd02808; GltS_FMN; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 2.
DR Gene3D; 2.160.20.60; Glutamate synthase, alpha subunit, C-terminal domain; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR002489; Glu_synth_asu_C.
DR InterPro; IPR036485; Glu_synth_asu_C_sf.
DR InterPro; IPR006982; Glu_synth_centr_N.
DR InterPro; IPR002932; Glu_synthdom.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR PANTHER; PTHR11938; FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE; 1.
DR PANTHER; PTHR11938:SF133; GLUTAMATE SYNTHASE (NADH); 1.
DR Pfam; PF00310; GATase_2; 1.
DR Pfam; PF04898; Glu_syn_central; 1.
DR Pfam; PF01645; Glu_synthase; 1.
DR Pfam; PF01493; GXGXG; 1.
DR SUPFAM; SSF69336; Alpha subunit of glutamate synthase, C-terminal domain; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 3: Inferred from homology;
KW 3Fe-4S {ECO:0000256|ARBA:ARBA00023291};
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Glutamate biosynthesis {ECO:0000256|ARBA:ARBA00023164};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:APU67681.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000186230}.
FT DOMAIN 18..413
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000259|PROSITE:PS51278"
SQ SEQUENCE 1507 AA; 168247 MW; 5D5976FE2E8AE9EC CRC64;
MKMKAQGLYS PDFERDNCGA GFICNLNGER TNQIIHKAID ILIRLEHRGA VSADGKTGDG
AGILIEVPHN FFKKVCDFEI PEFREYAVGM LFLPRNANQA DLCKKLFEEE VNNQQLEILG
WRKVPVNRSC LGKMASKSEP AVEQVFIARP EKLDDQHFNA KLYAARKIAE HAIESSGLAQ
SDYFYFSSLS TNTIIYKGLL MPQDINEYYK DLNDPDVITK LALVHQRFST NTFPTWDLAQ
PFRYMCHNGE INTLRGNLSR MRAREELFES ELFGQDLKKI VPITMEGKSD SASMDMALEM
LLQTGRSLPE AIMMMVPEAW EKNPSMDEKK KAFYAYNACI MEPWDGPASI PFTDGNYIGA
LLDRNGLRPS RYTVTKDGYV VMSSETGVLD IKPENVLKHG RLEPGRMFLV DMNEGRIVED
EEVKESIVSK RPYQEWLDEN LLALADVPYT GNQTPIEKEE YLTRLKLFGY TYEDITTIIS
PMAANGKEAI GSMGTDIPLA VLSHKPQLLF NYFKQLFAQV TNPPLDGIRE EIVTDISLPV
GEDLNLFDII PEQCRKLKIQ NPVISNEDLD KIKYIDQPGF KARSISMLYE ADKGMNGLED
RLEAMIYEIN DAVDDGCNVI ILSDRNVNPK LAPIPSLLAC SFVHHRVKDY NRRSSFGIVI
ESAEPREPHH FAALFGYGAS AINPYMVNEV IYQLVKEKQI PVEDPEEAVE NFNVAIGKGI
VKIMNKIGIS TLLSYRGSQI FEILGLNKKF VDKYFCNTPT RIEGIGLYEI EKEIQKRYKH
AFFPPETDTD LDLEMGGDYR WRRNGERHVF NPASVAKLQQ AVRQNSFETY SEYSKIINEQ
NENLMTIRGM FKFKDLNPIP LEEVEPWTEI VKRFKTGAMS FGSISKEAHE NLAIAMNRIK
GKSNSGEGGE DAGRFQKDIN GNWRNSAIKQ VASGRFGVSI DYLSNAREIQ IKMAQGAKPG
EGGQLPGPKV NPEIAKTRNS TPYVGLISPP PHHDIYSIED LAQLIFDLKN ANREARINVK
LVSKVGVGTI AAGVAKAKAD VVLISGYDGG TGASPLTSLR HAGLPWELGI AEAQQTLLLN
NLRSRIVVEC DGQLKTGRDV AIACLLGAEE FGFSTAPLVA SGCIMMRACH LNTCPVGIAT
QDPELRKNFK GTPENVINFM YFIAQEMRQI MAQLGFRSVA EMVGQSQKLD MNRAIKHYKA
QGIDLSNILH KPNIKDGVPL SNTEKQLHNL EGVLDFEILK QAHPAIYRKE PITLNYPIQN
VNRTTGAIIS NEISKIHGAK GLPDNTLTLN FTGSAGQSFG AFAARGLNLN IEGNSNDYFG
KGLSGAILSI RKPKEATFKS NENIIIGNVA LYGAITGEAY INGIGGERFC VRNSGAKAVI
EGIGDHGCEY MTGGIAVILG KIGRNFAAGM SGGTAYIFDP DNSIDRNNFN MEMIELEAPS
DENLQELEEM IVKHYQYTDS EVAREILENW EENSRAFIKV MPTEYKKALQ KMEAEKQKEE
HADLKTA
//
