ID   A0A1L7XCS4_9HELO        Unreviewed;       320 AA.
AC   A0A1L7XCS4;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=nitrilase {ECO:0000256|ARBA:ARBA00039045};
DE            EC=3.5.5.1 {ECO:0000256|ARBA:ARBA00039045};
GN   ORFNames=PAC_12693 {ECO:0000313|EMBL:CZR62796.1};
OS   Phialocephala subalpina.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC   Helotiales; Mollisiaceae; Phialocephala;
OC   Phialocephala fortinii species complex.
OX   NCBI_TaxID=576137 {ECO:0000313|EMBL:CZR62796.1, ECO:0000313|Proteomes:UP000184330};
RN   [1] {ECO:0000313|EMBL:CZR62796.1, ECO:0000313|Proteomes:UP000184330}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UAMH 11012 {ECO:0000313|EMBL:CZR62796.1,
RC   ECO:0000313|Proteomes:UP000184330};
RA   Ploux O.;
RL   Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a nitrile + 2 H2O = a carboxylate + NH4(+);
CC         Xref=Rhea:RHEA:21724, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29067; EC=3.5.5.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00036406};
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       Nitrilase family. {ECO:0000256|ARBA:ARBA00008129}.
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DR   EMBL; FJOG01000021; CZR62796.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1L7XCS4; -.
DR   STRING; 576137.A0A1L7XCS4; -.
DR   OrthoDB; 2785533at2759; -.
DR   Proteomes; UP000184330; Unassembled WGS sequence.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   CDD; cd07564; nitrilases_CHs; 1.
DR   Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR044149; Nitrilases_CHs.
DR   PANTHER; PTHR46044:SF1; CN HYDROLASE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR46044; NITRILASE; 1.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000184330}.
FT   DOMAIN          12..287
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000259|PROSITE:PS50263"
SQ   SEQUENCE   320 AA;  34582 MW;  CB930C895FC26E85 CRC64;
     MAPSAQDLKP KIKLAAVQSG PVFLNKAATT EKVCSLILEA GRNGADVIGF PEGFIPGHPG
     WVEVMPLSAE PAPSLFLELF KQAVEVPGPE SEAIGAACKE ANIFAVVGIN ERRPNTTGTL
     FNTQLFFGRD GTLLHKHQKF VPTVGERLVH APGETGSKSS VTTDFGTLSG FICGENGNPL
     AQYSLSLDYP VVHVASWPSH FCPGSDVQNA ILVITRGLAT SLMCYVINSV AVVGDDVVEK
     YGIDDATRKF LREEQKKRRA TIIAPGGVIA GPFEEAQEGI LYAEVHTDDL IKGKYGLDYA
     GHYNRPEIFA HHFKRYFGQK
//