ID   A0A1L8FFK7_XENLA        Unreviewed;       658 AA.
AC   A0A1L8FFK7;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 38.
DE   RecName: Full=methylenetetrahydrofolate reductase (NADPH) {ECO:0000256|ARBA:ARBA00034530};
DE            EC=1.5.1.53 {ECO:0000256|ARBA:ARBA00034530};
GN   Name=mthfr.S {ECO:0000313|RefSeq:XP_018083188.1,
GN   ECO:0000313|Xenbase:XB-GENE-17337407};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355 {ECO:0000313|Proteomes:UP000186698, ECO:0000313|RefSeq:XP_018083188.1};
RN   [1] {ECO:0000313|RefSeq:XP_018083188.1}
RP   IDENTIFICATION.
RC   STRAIN=J_2021 {ECO:0000313|RefSeq:XP_018083188.1};
RC   TISSUE=Erythrocytes {ECO:0000313|RefSeq:XP_018083188.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-
CC         methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH;
CC         Xref=Rhea:RHEA:19817, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:18608, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.5.1.53;
CC         Evidence={ECO:0000256|ARBA:ARBA00034452};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19819;
CC         Evidence={ECO:0000256|ARBA:ARBA00034452};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU004254}.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC       {ECO:0000256|ARBA:ARBA00006743}.
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DR   RefSeq; XP_018083188.1; XM_018227699.2.
DR   AlphaFoldDB; A0A1L8FFK7; -.
DR   STRING; 8355.A0A1L8FFK7; -.
DR   PaxDb; 8355-A0A1L8FFK7; -.
DR   GeneID; 108697556; -.
DR   KEGG; xla:108697556; -.
DR   AGR; Xenbase:XB-GENE-17337407; -.
DR   CTD; 108697556; -.
DR   Xenbase; XB-GENE-17337407; mthfr.S.
DR   OMA; GVFKTIP; -.
DR   OrthoDB; 1381745at2759; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000186698; Chromosome 7S.
DR   Bgee; 108697556; Expressed in egg cell and 18 other cell types or tissues.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00537; MTHFR; 1.
DR   Gene3D; 3.20.20.220; -; 1.
DR   InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR   InterPro; IPR004621; Fadh2_euk.
DR   InterPro; IPR003171; Mehydrof_redctse-like.
DR   NCBIfam; TIGR00677; fadh2_euk; 1.
DR   PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   Pfam; PF02219; MTHFR; 1.
DR   SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000186698}.
SQ   SEQUENCE   658 AA;  75258 MW;  4805602578D852DA CRC64;
     MWNSGPLAVR NCGLRMVNAN SSDSGTASCS SEENSKESSR CSTPVLDIER HERLKDKMKR
     RIEAGDRWFS LEFFPPRTAS GAVNLISRFD RMGSGGPLFI DVTWHPAGDP GSDKETSSMV
     IASTAVNFCG LESILHMTCC TQTKEDITKH LQKAKHLGLK NIMALRGDPT GEEWEEEAEG
     FNYAVDLVKH IRHEFDDYFD ICVAGYPKGH PEADSYDQDL KHLKEKIDAG ADFIITQLFF
     RSETFLTFLK DCRAIGITCP VLPGIFPIQG YGSLRQLVKL SKLEVPQEIM DIIEPIKDND
     AAIRNYGIDL AVSMCRELLD SGLVHGLHFY TLNREVATID VLKRLGMWKE DPRRPMPWAM
     SAHPKRRVED VRPIFWASRP KSYIHRTQEW DEFPNGRWGN SSSPAFGDLK DYYLFYLKSK
     SPREELLKMW GQELTSEESV FEVFTHYISG EPNPNGHKVT CLPWNDDPLA PETNLLKNEL
     QKVNKRGILT INSQPNVNGK PSTDPIMGWG PSRGYVFQKA YLEFFTSSEM VSALIKVLKR
     YEPRMNYHIV NVHGKNITNA ADLQPNAVTW GIFPGREIVQ PTVVDPVSFM FWKDEAFALW
     IEQWAKFYEE ESPSRMIIQY IHDNYYLVNL VDNDFPLENC LWQVIEDTFK EHDSPTDQ
//