ID   A0A1M3FZS9_9SPHI        Unreviewed;       653 AA.
AC   A0A1M3FZS9;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=Peptidase M1 {ECO:0000313|EMBL:OJW32922.1};
GN   ORFNames=BGO54_14810 {ECO:0000313|EMBL:OJW32922.1};
OS   Sphingobacteriales bacterium 46-32.
OC   Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales.
OX   NCBI_TaxID=1895839 {ECO:0000313|EMBL:OJW32922.1, ECO:0000313|Proteomes:UP000184358};
RN   [1] {ECO:0000313|EMBL:OJW32922.1, ECO:0000313|Proteomes:UP000184358}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=46-32 {ECO:0000313|EMBL:OJW32922.1};
RA   Kantor R.S., Huddy R.J., Iyer R., Thomas B.C., Brown C.T., Anantharaman K.,
RA   Tringe S., Hettich R.L., Harrison S.T., Banfield J.F.;
RT   "Genome-resolved meta-omics ties microbial dynamics to process performance
RT   in biotechnology for thiocyanate degradation.";
RL   Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRSR:PIRSR634015-3};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634015-3};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OJW32922.1}.
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DR   EMBL; MKTY01000020; OJW32922.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1M3FZS9; -.
DR   STRING; 1895839.BGO54_14810; -.
DR   Proteomes; UP000184358; Unassembled WGS sequence.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd09604; M1_APN_like; 1.
DR   Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR   InterPro; IPR014782; Peptidase_M1_dom.
DR   InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR   PANTHER; PTHR11533:SF303; AMINOPEPTIDASE N; 1.
DR   PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR   Pfam; PF01433; Peptidase_M1; 1.
DR   SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR634015-3};
KW   Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|PIRSR:PIRSR634015-3}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..653
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5012160171"
FT   DOMAIN          367..552
FT                   /note="Peptidase M1 membrane alanine aminopeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF01433"
FT   ACT_SITE        408
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   ACT_SITE        492
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   BINDING         407
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         411
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         430
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
SQ   SEQUENCE   653 AA;  74002 MW;  24CF9BC04048AA58 CRC64;
     MKCIAAAITA SIITLLASGQ SAFDAKEAFN PHFYPYPGNE MRSAGGEPGP GYWQNQASYR
     INCTLDTGQK RITGSVDIEY VNNSPDNLRF LWLQLDQNIY KKDSRGSATT TEAGGRWAND
     GYTSGYIITA VEAESAGGKY AARYLINDTR MQVWLQEPLQ AKGGKIKLSI PFAFTIPEYG
     TDRMGLLNSQ HGWIYQIAQW FPRLCVYDDI QGWNVDPYLG AGEFFLEYGH IEYAITAPAN
     LLVVGSGTLL NPSECFNAEE LVRYNQAKES DKTILIRSLK DVTAQVRNSR MGTITWKFSI
     ENARDVAWAA SGAFILDGAR INLPSGKRAL ALSAYPAESA REKKEEDWRK STEMVKASIE
     YYSGKWYEYP YTTAVNVAGS VGGMEYPGIV FCSYSSTGSS LWNVTDHEFG HTWFPMIVGS
     NERKYAWMDE GFNTFINDLS TEAFRNGVYN NANLFSNPSL PFMIQATFGD EMDAMFTSPD
     VIQQQNLGIA AYMKPAQMLH ALRDVVLGPE RFDAAFKEYI RRWAFKHPTP WDFFHTMENV
     GGEDLAWFWR GWIFNSWKLD QAVKEVKYVK NNPGNGAEIT IENKEKMPMP VDLLIKESNG
     KEHRLLLPVE VWQRGATWTI HLQVTSEIKE IIIDPDKKLP DWNRENNRWK RAF
//