ID A0A1M3GWH0_9PROT Unreviewed; 1078 AA.
AC A0A1M3GWH0;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BGO60_00230 {ECO:0000313|EMBL:OJW47302.1};
OS Thiobacillus sp. 65-1059.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Thiobacillaceae; Thiobacillus.
OX NCBI_TaxID=1895860 {ECO:0000313|EMBL:OJW47302.1, ECO:0000313|Proteomes:UP000184230};
RN [1] {ECO:0000313|EMBL:OJW47302.1, ECO:0000313|Proteomes:UP000184230}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=65-1059 {ECO:0000313|EMBL:OJW47302.1};
RA Kantor R.S., Huddy R.J., Iyer R., Thomas B.C., Brown C.T., Anantharaman K.,
RA Tringe S., Hettich R.L., Harrison S.T., Banfield J.F.;
RT "Genome-resolved meta-omics ties microbial dynamics to process performance
RT in biotechnology for thiocyanate degradation.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OJW47302.1}.
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DR EMBL; MKUE01000099; OJW47302.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1M3GWH0; -.
DR STRING; 1895860.BGO60_00230; -.
DR Proteomes; UP000184230; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF23; RECBCD ENZYME SUBUNIT RECB; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 1..472
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 498..787
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 21..28
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1078 AA; 116763 MW; 8EEA95C040C72B9F CRC64;
MSEPLETSVA LAPGHSAVVE ACAGSGKTWL LVSRMIRLLL AGAEPSELLA ITFTRKAAQE
MTDRLHAWLR VLALEDDATV RAFLRERAVP EHEIEALLPR ARALLETVLA AQPGPTVTTF
HGWFLDLLKR APLEAGLPWG APLLERERQL QDEVRDQLLT KWAVAPESAE GTALLALLAD
IGEHSLHALL AHFLQARAEW WAYTDGQPDP VAYALEQLRP GLHPDLASDP VAAFWADELM
LARVKRVGHA LEKGSKTERE RAVDILRSPT LDAESAHACL MTRLMTAGAR RKKQATRELE
KALGAELDAY LRDLDALETA LETITAIQTD IRIWNLNRHG LLLGHALLQG YQDAKAQQGA
IDFADAEWLA CRLLASEEHA PALALKLDAR YRHLLLDEFQ DTNPLQWQAL STWLSEARAA
DSAMTVFMVG DPKQAIYRFR RGEARVFDAA ADFLRTHFGA PHFSATMTRR LSPAVVQAIN
PVFAGVAGFA AHSHAPANAA RPGALRCLPV GVERSAAAAT AGLRNPLTTP QPEADDRAVH
VEAQAFARVL RDEMLGRWGV ADGPAGNSRP ARPGDVMALV RKRTHLHLYE RALEAAGIPF
ITSRRGGLLH ALEAQDVIAL LETLLLPHAD LKLAHALKSP IFGCSDADLP RVFGPAGPCG
WERLTALGGS PDSPPALARA AQLLLRWRAR VGTLPVHDLL DRIYFEGDVE ARYAAAVPPA
QRPQVAANLR AFMRLALTQD AGRYPTLAGF IRELASLVDD ADAAPGEGLA ADGENAVRLL
TIHGAKGLEA PIVWLLGGSD HARGDSHGVL APWPPEAARP EHFSLFGKQD ERGAWRERWF
AEEAALTQRE SDNLLYVALT RAEQGLIVSG AADKSAWLQR VEAAWQGLAL PADLPPAASA
DAPCMTALAP RIAAPAVGQR LAPAPASPAA ASGELFHACL EHHAPPGTPR DLPALAMQLG
VAAGLARIEA DARALLAQPH LARFFDPALY LRAHSELALL DADGRMQRLD RVVEFDDAVW
LIDYKTGDDS RMLSDAQLAE RHRPQLAGYR TLLAGLYPGK PVHAAVLLAD GRLIQVET
//
