ID   A0A1M3PU79_9PROT        Unreviewed;       429 AA.
AC   A0A1M3PU79;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   RecName: Full=TRAM domain-containing protein {ECO:0000259|Pfam:PF01938};
GN   ORFNames=BGP12_20710 {ECO:0000313|EMBL:OJY70188.1};
OS   Rhodospirillales bacterium 70-18.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales.
OX   NCBI_TaxID=1895826 {ECO:0000313|EMBL:OJY70188.1, ECO:0000313|Proteomes:UP000184399};
RN   [1] {ECO:0000313|EMBL:OJY70188.1, ECO:0000313|Proteomes:UP000184399}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-18 {ECO:0000313|EMBL:OJY70188.1};
RA   Kantor R.S., Huddy R.J., Iyer R., Thomas B.C., Brown C.T., Anantharaman K.,
RA   Tringe S., Hettich R.L., Harrison S.T., Banfield J.F.;
RT   "Genome-resolved meta-omics ties microbial dynamics to process performance
RT   in biotechnology for thiocyanate degradation.";
RL   Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OJY70188.1}.
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DR   EMBL; MKWE01000031; OJY70188.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1M3PU79; -.
DR   STRING; 1895826.BGP12_20710; -.
DR   Proteomes; UP000184399; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          12..45
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|Pfam:PF01938"
FT   ACT_SITE        387
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         263
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         293
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         313
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         361
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   429 AA;  44538 MW;  2D9DFAD179F0657C CRC64;
     MTAPAGAPAA AELRVSRIGS DGDGVGHLPD GIVVYLPGTL PGETVLAHPQ FKRGDGWAGT
     ADLLATSDDR AAPPCPHFGP CGGCALQHWR DDSYLAWKTA LLETALRRAG YGEMPAPAIR
     TPPGARRRVD LAIRRQGPAL LLGLHGKRSA DIVDLHACPV LHPDLFALIA PLRTLLRGLG
     GLRRHGSAIL NLLDSGPDLL LRTDAALTTA DRTKLAAFAQ AHGLPRITAA IGDGPHESAA
     LLRPPTTAMS GITVTPPPGA FLQASAEGEA AIIAAVLAGL PKLAGKARIA ELYAGCGTLT
     FALAKQARIA AFEGDIEAHA ALRQAANNSG LAGRVEALRR DLTRQPLSAK ELAAFGAVVL
     DPPHGGAVTQ AALIAASGVA RIIYVSCNPA TLGRDAKLFR DAGYKLLAAT PIDQFLWSPR
     LESVTVFAR
//