ID   A0A1M4N574_9CLOT        Unreviewed;       970 AA.
AC   A0A1M4N574;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 32.
DE   RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE            EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN   Name=gyrA_1 {ECO:0000313|EMBL:SCN21259.1};
GN   ORFNames=N3C_0035 {ECO:0000313|EMBL:SCN21259.1};
OS   Clostridium sp. N3C.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1776758 {ECO:0000313|EMBL:SCN21259.1, ECO:0000313|Proteomes:UP000184869};
RN   [1] {ECO:0000313|EMBL:SCN21259.1, ECO:0000313|Proteomes:UP000184869}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N3C {ECO:0000313|EMBL:SCN21259.1};
RA   Seilhamer J.J.;
RL   Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. {ECO:0000256|ARBA:ARBA00008263}.
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DR   EMBL; FMJL01000001; SCN21259.1; -; Genomic_DNA.
DR   RefSeq; WP_074364034.1; NZ_FMJL01000001.1.
DR   AlphaFoldDB; A0A1M4N574; -.
DR   STRING; 1776758.N3C_0035; -.
DR   OrthoDB; 9806486at2; -.
DR   Proteomes; UP000184869; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR   Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR   Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 6.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 2.
DR   SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:SCN21259.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184869};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          17..477
FT                   /note="DNA topoisomerase type IIA"
FT                   /evidence="ECO:0000259|SMART:SM00434"
FT   COILED          449..476
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   970 AA;  110030 MW;  C1FC6B3D873D51D5 CRC64;
     MVKKSSAIPK DRNIIKVPLE EAMPDNYLPY AVEVAKDRAL PDVRDGLKPV HRRILYGAYL
     LKATPDKPYY KSARIVGDIL GKFHPHGDAS VYDSMVILAQ DFTTRKPLID GHGNWGSIDG
     DSAAAMRYTE ARLTPIALEM IRDIDKNVVD MIPNYSESDM EPKVLPSRYP NILVNGAFGI
     AVGLATNIPP HNLREVIQGL LAYIDNPDIT TKELMNYIKG PDLPTGGVLI GAESLLSAYE
     TGIGKVTLRA KASIEKLESG KLGIVITEFP YRKNKAKLLQ TISEMTGDKK HAKVLDPIID
     IRDESDRNGI RAVIEFRKST DYETGDKILK YLYKKTDLQS NLSFNMVALA KGKPETLGLK
     AILYHYLQHQ KEVITRRTQT ELDIALKRFH IVEGFIKAID IMDQVIATIR ASKSKKDAQD
     NLVSKFGFTE LQAEAILELM LYRLTGLEIT VFQKEYKELE KRIKSLRKIL GDEQVLLQVI
     KDELIEIMEK YGDDRRTEII HDENEAKIDV EELIVIEDTM VTMSNEGFIK RVPQKSYNRS
     NADESEIEYR EGDYNKFLFP SNTRDTVLFF TDLGNMYQIK GIEIPEYKWR EKGEKLDTLI
     KGLDLNLEKI IAVFSFQELA ADKTLMLITN KGYIKRTSLN KFVTSYTKLL ALKLKKDERL
     IYVGLENPDV EINYIKLTSK LGLTYTVRKP ELDKVDRNVL GVQLCPIGGR DELVAIDFVD
     NYEFEPYTLA VSKEGLIKRL ASERHKSGYK LTVSYGQDIL IFSSSGLVYK FPYYLFENAE
     EGIELKNLVE EYDPKINKIL YVMVVDKYTE QNYIYFFSKK GLLKKTPLED FQGNYITTQA
     YKFKTATDSL VSITSSKQED SSAIIVTKKA MSIRFLVSSI NTMGKIASGV TGISLKEDDE
     VIFGGIINAG EKDSEVAVNS DTDGELILIS YKKQERRIKV EDIKIQNRAG RGNNLMLIVL
     DDYIKDVKIV
//