ID A0A1M4NDS4_9CLOT Unreviewed; 522 AA.
AC A0A1M4NDS4;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=N3C_2442 {ECO:0000313|EMBL:SCN25698.1};
OS Clostridium sp. N3C.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1776758 {ECO:0000313|EMBL:SCN25698.1, ECO:0000313|Proteomes:UP000184869};
RN [1] {ECO:0000313|EMBL:SCN25698.1, ECO:0000313|Proteomes:UP000184869}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N3C {ECO:0000313|EMBL:SCN25698.1};
RA Seilhamer J.J.;
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FMJL01000049; SCN25698.1; -; Genomic_DNA.
DR RefSeq; WP_074366286.1; NZ_FMJL01000049.1.
DR AlphaFoldDB; A0A1M4NDS4; -.
DR STRING; 1776758.N3C_2442; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000184869; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:SCN25698.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000184869};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:SCN25698.1}.
FT DOMAIN 13..164
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 178..486
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 522 AA; 59233 MW; C87D24AAE997DC85 CRC64;
MENNRKEQER AELHRTIWNM ANDLRGSVDG WDFKQYVLGM LFYRYISENI TAYINAGEWE
AGNTEFDYAK LSDEEAEQAR EDLVKTKGFF ILPSELFENV RARAKDDENL NETLEQIFSN
IEASAQGTES EDNFKGLFDD IDVNSNKLGN TVVKRNEKLV KLMNSVGEMK LGDYKDNTID
AFGDAYEFLM GMYASNAGKS GGEYYTPQEV SELLTHLTLV GKTEVNKVYD PACGSGSLLL
KFAKILGKEN VRQGFFGQEI NITTYNLCRI NMFLHDIDYD KFDIALGDTL TDPQHWDDEP
FEAIVSNPPY SIKWKGDNDP ILINDPRFSP AGVLAPKSKA DLAFIMHSLS WLATNGTAAI
VCFPGVMYRG GAEKKIRQYL IDNNYIDCII QLPDNLFYGT SIATCIMVLK KSKSENSTLF
IDASKEFVKV TNNNKLTQEN IETILNAFKD RKDIEHFARL VPNSEIAEQD YNLSVSTYVE
QEDTREKIDI VALNAEIEKI VAREQILREE IDKIIAEIEV GK
//
