ID A0A1M4URZ4_9GAMM Unreviewed; 564 AA.
AC A0A1M4URZ4;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Fanconi-associated nuclease {ECO:0000256|RuleBase:RU365033};
DE EC=3.1.4.1 {ECO:0000256|RuleBase:RU365033};
GN ORFNames=SAMN04487965_0236 {ECO:0000313|EMBL:SHE59455.1};
OS Microbulbifer donghaiensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
OC Microbulbiferaceae; Microbulbifer.
OX NCBI_TaxID=494016 {ECO:0000313|EMBL:SHE59455.1, ECO:0000313|Proteomes:UP000184170};
RN [1] {ECO:0000313|EMBL:SHE59455.1, ECO:0000313|Proteomes:UP000184170}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.7063 {ECO:0000313|EMBL:SHE59455.1,
RC ECO:0000313|Proteomes:UP000184170};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nuclease required for the repair of DNA interstrand cross-
CC links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of
CC DNA and cleaves DNA successively at every third nucleotide, allowing to
CC excise an ICL from one strand through flanking incisions.
CC {ECO:0000256|RuleBase:RU365033}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolytically removes 5'-nucleotides successively from the
CC 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.;
CC EC=3.1.4.1; Evidence={ECO:0000256|ARBA:ARBA00000983,
CC ECO:0000256|RuleBase:RU365033};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365033}.
CC -!- SIMILARITY: Belongs to the FAN1 family. {ECO:0000256|ARBA:ARBA00005533,
CC ECO:0000256|RuleBase:RU365033}.
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DR EMBL; FQVA01000001; SHE59455.1; -; Genomic_DNA.
DR RefSeq; WP_073270651.1; NZ_FQVA01000001.1.
DR AlphaFoldDB; A0A1M4URZ4; -.
DR STRING; 494016.SAMN04487965_0236; -.
DR OrthoDB; 9803913at2; -.
DR Proteomes; UP000184170; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004528; F:phosphodiesterase I activity; IEA:UniProtKB-EC.
DR GO; GO:0036297; P:interstrand cross-link repair; IEA:InterPro.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR033315; Fan1-like.
DR InterPro; IPR049125; FAN1-like_WH.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR014883; VRR_NUC.
DR PANTHER; PTHR15749; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR PANTHER; PTHR15749:SF4; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR Pfam; PF21315; FAN1_HTH; 1.
DR Pfam; PF08774; VRR_NUC; 1.
DR SMART; SM00990; VRR_NUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU365033};
KW DNA repair {ECO:0000256|RuleBase:RU365033};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU365033};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365033};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|RuleBase:RU365033};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365033};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU365033};
KW Nucleus {ECO:0000256|RuleBase:RU365033}.
FT DOMAIN 448..561
FT /note="VRR-NUC"
FT /evidence="ECO:0000259|SMART:SM00990"
SQ SEQUENCE 564 AA; 64434 MW; C154EE606F7F21E4 CRC64;
MGEPVELAPD YYLSNFRALV DFVVARYEGL LSDRERRFYH DFRALELNSQ RLYVRLLSRK
GVPSSRGALF RLGKLNYVEI ENLPLAAEQL VTADLLQRDP ALALEEMLPL FSKAELQACS
PVPLPKALKR PALELALLEL HDEAPALRNA LAAGETLLAV QAAEHFETFK LCFFGNLNQD
LTDYVLRDLG MYRYENYPLD RKNLPFQSRA QIDRHLRYYD CLELLDGALE AGPEEILALA
EQLPEVSDGD ATLHRRLDRL RLTLARQLER LEALDAADRL YRACARPPAR ERRARIAVTR
GDIDTGLALC GEILAAPHNE AEQVFAESFG YRTAKKAKQL GGWQPPERCK PPTETVVLPP
APDRVELLAA AYLELGSPSA EPDPFPHQCF YVENTLFNGV LGLYVWDILF SPVPGAFFNP
FQVAPSDFRT GDFFLQRRVA FEQRLAELDV EHLARRVWRH YRQKYGVANP LVAWEALPEP
LLQLALERIP ADHWHRLFRR LLSDIGHHRN GLPDLILFPS SGGYELVEVK GPGDRLQQNQ
QRWLAYFARH QIPHRVLHVE WQQP
//
