ID A0A1M4WXJ0_9BACL Unreviewed; 656 AA.
AC A0A1M4WXJ0;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Cyclic-di-AMP phosphodiesterase {ECO:0000256|PIRNR:PIRNR026583};
DE EC=3.1.4.- {ECO:0000256|PIRNR:PIRNR026583};
GN ORFNames=SAMN05444392_10419 {ECO:0000313|EMBL:SHE85956.1};
OS Seinonella peptonophila.
OC Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
OC Seinonella.
OX NCBI_TaxID=112248 {ECO:0000313|EMBL:SHE85956.1, ECO:0000313|Proteomes:UP000184476};
RN [1] {ECO:0000313|EMBL:SHE85956.1, ECO:0000313|Proteomes:UP000184476}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44666 {ECO:0000313|EMBL:SHE85956.1,
RC ECO:0000313|Proteomes:UP000184476};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Has phosphodiesterase (PDE) activity against cyclic-di-AMP
CC (c-di-AMP). {ECO:0000256|PIRNR:PIRNR026583}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',3'-c-di-AMP + H2O = 5'-O-phosphonoadenylyl-(3'->5')-
CC adenosine + H(+); Xref=Rhea:RHEA:54420, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:71500, ChEBI:CHEBI:138171;
CC Evidence={ECO:0000256|PIRNR:PIRNR026583};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|PIRNR:PIRNR026583}.
CC -!- SIMILARITY: Belongs to the GdpP/PdeA phosphodiesterase family.
CC {ECO:0000256|PIRNR:PIRNR026583}.
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DR EMBL; FQVL01000004; SHE85956.1; -; Genomic_DNA.
DR RefSeq; WP_073154426.1; NZ_FQVL01000004.1.
DR AlphaFoldDB; A0A1M4WXJ0; -.
DR STRING; 112248.SAMN05444392_10419; -.
DR OrthoDB; 9759476at2; -.
DR Proteomes; UP000184476; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0106409; F:cyclic-di-AMP phosphodiesterase activity; IEA:RHEA.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.310.30; -; 1.
DR Gene3D; 3.90.1640.10; inorganic pyrophosphatase (n-terminal core); 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR InterPro; IPR001667; DDH_dom.
DR InterPro; IPR038763; DHH_sf.
DR InterPro; IPR003156; DHHA1_dom.
DR InterPro; IPR049553; GdpP-like_PAS.
DR InterPro; IPR014528; GdpP/PdeA.
DR PANTHER; PTHR47618; BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA; 1.
DR PANTHER; PTHR47618:SF2; CYCLIC-DI-AMP PHOSPHODIESTERASE GDPP; 1.
DR Pfam; PF01368; DHH; 1.
DR Pfam; PF02272; DHHA1; 1.
DR Pfam; PF21370; GdpP_PAS; 1.
DR PIRSF; PIRSF026583; YybT; 1.
DR SUPFAM; SSF64182; DHH phosphoesterases; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|PIRNR:PIRNR026583};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR026583};
KW Membrane {ECO:0000256|PIRNR:PIRNR026583, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000184476};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..29
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 75..161
FT /note="Cyclic-di-AMP phosphodiesterase GdpP-like PAS"
FT /evidence="ECO:0000259|Pfam:PF21370"
FT DOMAIN 339..495
FT /note="DDH"
FT /evidence="ECO:0000259|Pfam:PF01368"
FT DOMAIN 517..645
FT /note="DHHA1"
FT /evidence="ECO:0000259|Pfam:PF02272"
SQ SEQUENCE 656 AA; 75323 MW; 1DACB68AB7C667DF CRC64;
MPKFTKRYRH SYLLLFFLWL IFLVFIYHFT SNWWLTGLGG GLWFLSLLFV WLQGRLYQDE
WRRALLLIQR RVIQGHRFAI HHLPYGLVLI NDDGVIEWHN QYVSKKMNVS TSSIGKVVDQ
VFPLDLEHLR NPATVSEMAL GESTYEVSYD SKHQFLLFRD ITELVQLEEK YEQEQPIVGY
LYIDNYDEVR QQLTDQEETI LLNTVNSMIS AWTERYQISL KRFDVDKMFF VTQALYLRKL
IESHFDILDE MRNATHDQPI PLTLSMGVSN STGSVLDRAK TARAALNVAL ARGGDQTAVQ
EKERLVFFGG KTNAIERRTR VRARVISHAM SNLFRANRRV VIMGHMNPDL DAIGAAVGVA
CFAQYHQCEP HIILNESNPS IDRVWKKIKE YPTIESLFSL ASEVEDWIHE RDTLLVMVDT
HRPSLTIEPK LLERVRHIVV IDHHRRGEDF VEDPILVYLE PYASSACELV TELLQYQDEQ
CEMSAFEATL LLAGIVVDTK HFAFRTGSRT FEAASFLREK GADLALVQSL LKEDLDQYIK
RAEMLKQTEM WGDEVAVAVG KEDEVYDQLL IAQAADTLLN MKGVAASFVI AKRKDGLVAI
SARSQGEINV QLIMEAMGGG GHFTNAASQM KDCSLEETKE ILKKVVEKYS DREEKD
//
