ID   A0A1M4XCE7_9FIRM        Unreviewed;       557 AA.
AC   A0A1M4XCE7;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   24-JAN-2024, entry version 28.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN   ORFNames=SAMN02746064_01472 {ECO:0000313|EMBL:SHE91219.1};
OS   Alkalibacter saccharofermentans DSM 14828.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC   Alkalibacter.
OX   NCBI_TaxID=1120975 {ECO:0000313|EMBL:SHE91219.1, ECO:0000313|Proteomes:UP000184251};
RN   [1] {ECO:0000313|EMBL:SHE91219.1, ECO:0000313|Proteomes:UP000184251}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14828 {ECO:0000313|EMBL:SHE91219.1,
RC   ECO:0000313|Proteomes:UP000184251};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR   EMBL; FQTU01000009; SHE91219.1; -; Genomic_DNA.
DR   RefSeq; WP_073270648.1; NZ_FQTU01000009.1.
DR   AlphaFoldDB; A0A1M4XCE7; -.
DR   STRING; 1120975.SAMN02746064_01472; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000184251; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:SHE91219.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW   One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW   Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000184251}.
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   557 AA;  59877 MW;  5CF16F99601C7EF3 CRC64;
     MGFKSDIEIA QEAAPKDIRE IAAKLGLNEE NLDLYGKYKA KVDINLLQEK SDKRAKLILT
     TAINPTPAGE GKTTTTIGAA DALSRLGKNV LVALREPSLG PVFGVKGGAA GGGYAQVVPM
     EDINLHFTGD FHAIGAANNL LAAMLDNHVF QGNNLDIDVR RVVWRRAVDM NDRQLRNIVN
     GLGGKAQGMP REDGFDITVA SEIMAIFCLS SDIINLKNRC AKIIVAYNRK GEPVTAGDLN
     AQGAMAALLK DALKPNLVQT LEGNPAFIHG GPFANIAHGC NSVLATKFAM KYADYVVTEA
     GFGADLGAEK FINIKCRMTD IKPDAVIIVA TVKALKYNGG VAKTDLQEEN LVALEKGLPN
     LLKHVENITQ VFKLPAVVAI NRFPTDTEAE LKLVEDRCRE LGVNVALSEV WGKGGAGGEA
     LAKELIRLTQ TESDMEFAYD EEDSIKDKIR KVAQKIYGAD DVSFTGKAEK EIANFEKLGF
     GKMPICMAKT QYSLTDDQTI LGRPKGFTIT VRDLTVSAGA GFIVVLTGDV MKMPGLPKVP
     SAEKIDVDEN GVISGLF
//