ID A0A1M4Y223_9CLOT Unreviewed; 657 AA.
AC A0A1M4Y223;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=SAMN05443638_12311 {ECO:0000313|EMBL:SHE99613.1};
OS Clostridium fallax.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1533 {ECO:0000313|EMBL:SHE99613.1, ECO:0000313|Proteomes:UP000184035};
RN [1] {ECO:0000313|EMBL:SHE99613.1, ECO:0000313|Proteomes:UP000184035}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2631 {ECO:0000313|EMBL:SHE99613.1,
RC ECO:0000313|Proteomes:UP000184035};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; FQVM01000023; SHE99613.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1M4Y223; -.
DR SMR; A0A1M4Y223; -.
DR STRING; 1533.SAMN05443638_12311; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000184035; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000184035}.
FT DOMAIN 157..562
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 657 AA; 75134 MW; C9FDE24435A230E3 CRC64;
MKVLKEDILK SIKDKKDLSP IYESNEFEKN YFYNGDLGII YSEDKTIFKL WSPVAENVNI
AFYSGGLGER LIKIESMEKS EKGLWTFEAK GDLKGQFYNY LITINGEERE AVDPYAKAVG
VNGFRGAIIN LEDTNPTDWI YDSKPELKSI NDSIIYELHV RDFSIDEDSG MINKGKFLAF
TEENTKNSLG NSTGISHLKE LGITHIHLLP CYDFNSIDES KSLDSQYNWG YDPENYNVPE
GSYSTNPYNP ETRIIEFKKA IKSLHDNGIR VIMDVVYNHT EKTEESNFHK VVPYYYHRQN
SDGSFSNGSG CSNETASNRK MVRKFIIDSL TYWAKEYHID GFRFDLMAIH DIETMNKIRE
ELNKIDSSII IYGEGWNGGP STLPENKRAS KANVLKLDNN IAAFSDDIRD GVKGHVFYEK
DGGFVNGLGF EETIKFGVVA STEHPQINYS KINYSNNPWA KEPSQCINYV SAHDNLTLWD
KISITGKNLS IEDKIKMDKL SNAIVLTSQG IPFIHSGEEF LRTKYGDENS YKSGDKVNKI
DWKRKNQFKE VFNYYRGLIA LRKKYSIFRM KTNEDIKSCI SFFDTNENNL VAYSLKNKNE
NFIVIYNGNN KEVEISIPFG NWGVLVNENK AGLDILQYIK DDRIKVGSIS ALVIKSI
//
